Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
May 2012 Volume 3 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May 2012 Volume 3 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells

  • Authors:
    • Pengcheng He
    • Yanfeng Liu
    • Mei Zhang
    • Xiaoning Wang
    • Jieying Xi
    • Di Wu
    • Jing Li
    • Yunxin Cao
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Immunology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 776-780
    |
    Published online on: February 16, 2012
       https://doi.org/10.3892/etm.2012.488
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In order to investigate the effect and mechanisms of interferon (IFN)-γ in combination with all-trans-retinoic acid (ATRA) on NB4 cells [ATRA-sensitive acute promyelocytic leukemia (APL) cell line] and NB4-R1 cells (ATRA-resistant APL cell line) and to search for a novel approach to solve the problem of ATRA resistance in APL, we initially treated NB4 and NB4-R1 cells with IFN-γ, ATRA and IFN-γ in combination with ATRA, respectively. The cell proliferation was then tested by MTT assay, and the cell differentiation was tested through light microscopy, by NBT test and flow cytometry (FCM). The expression of promyelocytic leukemia (PML) protein was observed by indirect immune fluorescent test. Results showed that ATRA inhibited the growth of NB4 cells, however, it could not inhibit the growth of NB4-R1 cells. IFN-γ inhibited the growth of both NB4 and NB4-R1 cells. Meanwhile, the growth inhibition effect of IFN-γ in combination with ATRA on both NB4 and NB4-R1 cells was significantly stronger than that of any single drug treatment. The results of the NBT reduction test and CD11b antigen detection by FCM indicated that IFN-γ induces the differentiation of NB4 and NB4-R1 cells to some extent. Moreover, the maturation degree of both NB4 and NB4-R1 cells induced by IFN-γ in combination with ATRA was more significant than that of IFN-γ or ATRA alone. After treatment with IFN-γ, the number of fluorescent particles in NB4 and NB4-R1 cell nuclei was higher than those in the control group, which indicated that IFN-γ may induce the expression of PML protein. Together, IFN-γ augments the proliferation inhibition effect of ATRA on NB4 and NB4-R1 cells through enhancing the expression of PML protein. IFN-γ in combination with ATRA not only strengthens the induction differentiation effect of ATRA on NB4 cells, but also can partially induce the maturation of NB4-R1 cells with ATRA resistance.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1. 

Wang Z and Chen Z: Acute promyelocytic leukemia: from highly fatal to highly curable. Blood. 111:2505–2515. 2008. View Article : Google Scholar : PubMed/NCBI

2. 

Zhou GB, Zhang J, Wang ZY, Chen SJ and Chen Z: Treatment of acute promyelocytic leukaemia with all-trans retinoic acid and arsenic trioxide: a paradigm of synergistic molecular targeting therapy. Philos Trans R Soc Lond B Biol Sci. 362:959–971. 2007. View Article : Google Scholar : PubMed/NCBI

3. 

Wang X, Yin Y, Ma P, et al: Apoptosis of human pancreatic carcinoma cells induced by all-trans retinoic acid and interferon. Chin J Cancer Res. 21:224–228. 2009. View Article : Google Scholar

4. 

Park HH, Kim M, Lee BH, et al: Intracellular IL-4, IL-10, and IFN-gamma levels of leukemic cells and bone marrow T cells in acute leukemia. Ann Clin Lab Sci. 36:7–15. 2006.PubMed/NCBI

5. 

Hoyer KK, Herling M, Bagrintseva K, et al: T cell leukemia-1 modulates TCR signal strength and IFN-gamma levels through phosphatidylinositol 3-kinase and protein kinase C pathway activation. J Immunol. 175:864–873. 2005. View Article : Google Scholar : PubMed/NCBI

6. 

Montoro E, Lemus D, Echemendia M, et al: Comparative evaluation of the nitrate reduction assay, the MTT test, and the resazurin microtitre assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis. J Antimicrob Chemother. 55:500–505. 2005. View Article : Google Scholar : PubMed/NCBI

7. 

Huang Y, Liu J, Wang LZ, Zhang WY and Zhu XZ: Neuroprotective effects of cyclooxygenase-2 inhibitor celecoxib against toxicity of LPS-stimulated macrophages toward motor neurons. Acta Pharmacol Sin. 26:952–958. 2005. View Article : Google Scholar : PubMed/NCBI

8. 

Owen J, Bates J and Lewis S: Differential effects of nitroblue tetrazolium on the hemodynamic responses elicited by activation of alpha(1)-adrenoceptors and 5-HT(2) receptors in conscious rats. Eur J Pharmacol. 535:248–252. 2006. View Article : Google Scholar : PubMed/NCBI

9. 

Choi HS, Kim JW, Cha YN and Kim C: A quantitative nitroblue tetrazolium assay for determining intracellular superoxide anion production in phagocytic cells. J Immunoassay Immunochem. 27:31–44. 2006. View Article : Google Scholar : PubMed/NCBI

10. 

Shi J, Zhang Y, Su JY, et al: Cytotoxicity of IFN-gamma-activated dendritic cells to freshly isolated acute myeloid leukemia cells. J Exp Hematol. 13:1071–1075. 2005.PubMed/NCBI

11. 

Burchert A, Muller MC, Kostrewa P, et al: Sustained molecular response with interferon alfa maintenance after induction therapy with imatinib plus interferon alfa in patients with chronic myeloid leukemia. J Clin Oncol. 28:1429–1435. 2010. View Article : Google Scholar : PubMed/NCBI

12. 

Khoo TL, Vangsted AJ, Joshua D and Gibson J: Interferon-alpha in the treatment of multiple myeloma. Curr Drug Targets. 12:437–446. 2011.PubMed/NCBI

13. 

Benz R, Stussi G and Fehr J: Interferon as an alternative to purine analogues in the treatment of hairy cell leukaemia. Brit J Haematol. 148:664–666. 2010. View Article : Google Scholar : PubMed/NCBI

14. 

Gupta V, Yi QL, Brandwein J, et al: Role of all-trans-retinoic acid (ATRA) in the consolidation therapy of acute promyelocytic leukaemia (APL). Leukemia Res. 29:113–114. 2005. View Article : Google Scholar : PubMed/NCBI

15. 

Buonamici S, Li D, Mikhail F, et al: EVI1 abrogates interferon-alpha response by selectively blocking PML induction. J Biol Chem. 280:428–436. 2005. View Article : Google Scholar : PubMed/NCBI

16. 

Reineke EL and Kao HY: Targeting promyelocytic leukemia protein: a means to regulating PML nuclear bodies. Int Biol Sci. 5:366–376. 2009. View Article : Google Scholar : PubMed/NCBI

17. 

Chelbi-Alix MK, Vidy A, El Bougrini J and Blondel D: Rabies viral mechanisms to escape the IFN system: the viral protein P interferes with IRF-3, Stat1, and PML nuclear bodies. J Interferon Cytokine Res. 26:271–280. 2006. View Article : Google Scholar : PubMed/NCBI

18. 

Crowder C, Dahle O, Davis RE, Gabrielsen OS and Rudikoff S: PML mediates IFN-alpha-induced apoptosis in myeloma by regulating TRAIL induction. Blood. 105:1280–1287. 2005. View Article : Google Scholar : PubMed/NCBI

19. 

Dror N, Rave-Harel N, Burchert A, et al: Interferon regulatory factor-8 is indispensable for the expression of promyelocytic leukemia and the formation of nuclear bodies in myeloid cells. J Biol Chem. 282:5633–5640. 2007. View Article : Google Scholar : PubMed/NCBI

20. 

Bernardi R, Papa A and Pandolfi PP: Regulation of apoptosis by PML and the PML-NBs. Oncogene. 27:6299–6312. 2008. View Article : Google Scholar : PubMed/NCBI

21. 

Li L and He DL: Transfection of promyelocytic leukemia in retrovirus vector inhibits growth of human bladder cancer cells. Acta Pharmacol Sin. 26:610–615. 2005. View Article : Google Scholar : PubMed/NCBI

22. 

Ravindranath Y, Gregory J and Feusner J: Treatment of acute promyelocytic leukemia by using all-trans retinoic acid (ATRA). Leukemia. 18:1576–1577. 2004.

23. 

Gupta V, Yib QL, Brandwein J, et al: Clinico-biological features and prognostic significance of PML/RARalpha isoforms in adult patients with acute promyelocytic leukemia treated with all trans retinoic acid (ATRA) and chemotherapy. Leuk Lymphoma. 45:469–480. 2004. View Article : Google Scholar

24. 

Zhang X, Yan X, Zhou Z, et al: Arsenic trioxide controls the fate of the PML-RARα oncoprotein by directly binding PML. Science. 328:240–243. 2010.PubMed/NCBI

25. 

Wang ZG, Delva L, Gaboli M, et al: Role of PML in cell growth and the retinoic acid pathway. Science. 279:1547–1551. 1998. View Article : Google Scholar : PubMed/NCBI

26. 

Abreu ELR, Baruffi MR, de Lima AS, et al: The co-expression of PML/RAR alpha and AML1/ETO fusion genes is associated with ATRA resistance. Brit J Haematol. 128:407–409. 2005. View Article : Google Scholar : PubMed/NCBI

27. 

Mueller BU, Pabst T, Fos J, et al: ATRA resolves the differentiation block in t(15;17) acute myeloid leukemia by restoring PU. 1 expression. Blood. 107:3330–3338. 2006. View Article : Google Scholar : PubMed/NCBI

28. 

Andrianifahanana M, Agrawal A, Singh AP, et al: Synergistic induction of the MUC4 mucin gene by interferon-gamma and retinoic acid in human pancreatic tumour cells involves a reprogramming of signalling pathways. Oncogene. 24:6143–6154. 2005. View Article : Google Scholar

29. 

Liu CH: Study on the synergic effect of all-trans retinoic acid combined with IFN-alpha on the proliferation and differentiation of HL-60 cells. Chin J Cell Mol Immunol. 21:637–639. 642:2005.PubMed/NCBI

30. 

Gu ZM, Wu YL, Zhou MY, et al: Pharicin B stabilizes retinoic acid receptor-alpha and presents synergistic differentiation induction with ATRA in myeloid leukemic cells. Blood. 116:5289–5297. 2010. View Article : Google Scholar : PubMed/NCBI

31. 

Wang J, Peng Y, Sun YW, et al: All-trans retinoic acid induces XAF1 expression through an interferon regulatory factor-1 element in colon cancer. Gastroenterology. 130:747–758. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
He P, Liu Y, Zhang M, Wang X, Xi J, Wu D, Li J and Cao Y: Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells. Exp Ther Med 3: 776-780, 2012.
APA
He, P., Liu, Y., Zhang, M., Wang, X., Xi, J., Wu, D. ... Cao, Y. (2012). Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells. Experimental and Therapeutic Medicine, 3, 776-780. https://doi.org/10.3892/etm.2012.488
MLA
He, P., Liu, Y., Zhang, M., Wang, X., Xi, J., Wu, D., Li, J., Cao, Y."Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells". Experimental and Therapeutic Medicine 3.5 (2012): 776-780.
Chicago
He, P., Liu, Y., Zhang, M., Wang, X., Xi, J., Wu, D., Li, J., Cao, Y."Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells". Experimental and Therapeutic Medicine 3, no. 5 (2012): 776-780. https://doi.org/10.3892/etm.2012.488
Copy and paste a formatted citation
x
Spandidos Publications style
He P, Liu Y, Zhang M, Wang X, Xi J, Wu D, Li J and Cao Y: Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells. Exp Ther Med 3: 776-780, 2012.
APA
He, P., Liu, Y., Zhang, M., Wang, X., Xi, J., Wu, D. ... Cao, Y. (2012). Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells. Experimental and Therapeutic Medicine, 3, 776-780. https://doi.org/10.3892/etm.2012.488
MLA
He, P., Liu, Y., Zhang, M., Wang, X., Xi, J., Wu, D., Li, J., Cao, Y."Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells". Experimental and Therapeutic Medicine 3.5 (2012): 776-780.
Chicago
He, P., Liu, Y., Zhang, M., Wang, X., Xi, J., Wu, D., Li, J., Cao, Y."Interferon-γ enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with all-trans‑retinoic acid to induce maturation of NB4 and NB4-R1 cells". Experimental and Therapeutic Medicine 3, no. 5 (2012): 776-780. https://doi.org/10.3892/etm.2012.488
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team