Zoledronate inhibits phosphate and bone morphogenetic protein 2-induced extracellular calcification of vascular smooth muscle cells in vitro

  • Authors:
    • M.L. Hu
    • Y. Huang
    • Z.H. Zheng
    • Y. Lei
    • R.J. Liu
    • X.H. Wang
    • B. Lindholm
    • X.Q. Yu
  • View Affiliations

  • Published online on: March 1, 2012     https://doi.org/10.3892/etm.2012.501
  • Pages: 841-844
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Abstract

The aim of this study was to explore the effects of the bisphosphonate zoledronate on calcification induced by inorganic phosphate (Pi) and/or bone morphogenetic protein 2 (BMP-2) and the underlying mechanisms. Primary vascular smooth muscle cells (VSMCs) from rats were treated with 3 mM Pi or 3 mM Pi/BMP-2, with and without addition of zoledronate; 1.4 mM Pi served as a control. Calcium deposits, expression of core binding factor α-1 (Cbfa-1), osteopontin (OPN), parathyroid pituitary-specific transcription factor (Pit)-1 and Pit-2, and Pi uptake of VSMCs was determined. The calcification of VSMCs induced by elevated Pi or Pi/­BMP-2 was significantly inhibited by zoledronate. The expression of Cbfa-1, OPN and Pit-1 was increased significantly after treatment with an elevated level of Pi or Pi/BMP-2, and this expression was significantly suppressed by addition of zoledronate. Pi uptake of VSMCs increased following treatment with elevated Pi and significantly decreased by addition of zoledronate. These results indicated that zoledronate effectively inhibited calcification induced by Pi/BMP-2, and this may have been achieved by means of the downregulation of expression of calcification-related proteins and uptake of Pi.
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May 2012
Volume 3 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hu M, Huang Y, Zheng Z, Lei Y, Liu R, Wang X, Lindholm B and Yu X: Zoledronate inhibits phosphate and bone morphogenetic protein 2-induced extracellular calcification of vascular smooth muscle cells in vitro. Exp Ther Med 3: 841-844, 2012.
APA
Hu, M., Huang, Y., Zheng, Z., Lei, Y., Liu, R., Wang, X. ... Yu, X. (2012). Zoledronate inhibits phosphate and bone morphogenetic protein 2-induced extracellular calcification of vascular smooth muscle cells in vitro. Experimental and Therapeutic Medicine, 3, 841-844. https://doi.org/10.3892/etm.2012.501
MLA
Hu, M., Huang, Y., Zheng, Z., Lei, Y., Liu, R., Wang, X., Lindholm, B., Yu, X."Zoledronate inhibits phosphate and bone morphogenetic protein 2-induced extracellular calcification of vascular smooth muscle cells in vitro". Experimental and Therapeutic Medicine 3.5 (2012): 841-844.
Chicago
Hu, M., Huang, Y., Zheng, Z., Lei, Y., Liu, R., Wang, X., Lindholm, B., Yu, X."Zoledronate inhibits phosphate and bone morphogenetic protein 2-induced extracellular calcification of vascular smooth muscle cells in vitro". Experimental and Therapeutic Medicine 3, no. 5 (2012): 841-844. https://doi.org/10.3892/etm.2012.501