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Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma

  • Authors:
    • Hua Zhong
    • Lan Xu
    • Ji-Hua Zhong
    • Fei Xiao
    • Qiang Liu
    • Hong-Hui Huang
    • Fang-Yuan Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Renji Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200127, P.R. China, Department of Pathology, Renji Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200127, P.R. China
  • Pages: 763-770
    |
    Published online on: March 2, 2012
       https://doi.org/10.3892/etm.2012.502
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Abstract

It has been found that aberrant expression of microRNAs (miRNAs) is strongly associated with carcinogenesis. In the present study, we investigated the expression of miR-155 and miR-146a in diffuse large B-cell lymphoma (DLBCL) patients (n=90). The expression levels of miR-155 and miR-146a were significantly higher in de novo DLBCL patients. miR-146a expression levels were associated with miR-155, lactate dehydrogenase, β 2 microglobulin, c-myc, International Prognostic Index status and Eastern Cooperative Oncology Group performance status. We found that patients with low miR-155 and miR-146a expression levels achieved a higher complete remission rate, higher overall response rate and longer progression-free survival time. Moreover, a high expression level of miR-155, but not miR-146a, was an independent indicator for chemotherapy protocol selection in our study. Patients with high expression of miR-155 received more survival benefits from rituximab treatment. These data suggest that miR-155 and miR-146a have potential as diagnostic and prognostic markers in DLBCL.
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1. 

Chen K and Rajewsky N: The evolution of gene regulation by transcription factors and microRNAs. Nat Rev Genet. 8:93–103. 2007. View Article : Google Scholar : PubMed/NCBI

2. 

Kim VN: MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol. 6:376–385. 2005. View Article : Google Scholar : PubMed/NCBI

3. 

Calin GA, Sevignani C, Dumitru CD, et al: Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci USA. 101:2999–3004. 2004. View Article : Google Scholar : PubMed/NCBI

4. 

Lawrie CH: MicroRNA expression in lymphoid malignancies: new hope for diagnosis and therapy? Cell Mol Med. 12:1432–1444. 2008. View Article : Google Scholar : PubMed/NCBI

5. 

Calin GA, Ferracin M, Cimmino A, et al: A microRNA signature associated with prognosis and progression in chronic lymphocytic leukemia. N Engl J Med. 353:1793–1801. 2005. View Article : Google Scholar : PubMed/NCBI

6. 

Lawrie CH, Gal S, Dunlop HM, et al: Detection of elevated levels of tumour associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol. 141:672–675. 2008. View Article : Google Scholar : PubMed/NCBI

7. 

Kota SK and Balasubramanian S: Cancer therapy via modulation of microRNA levels: a promising future. Drug Discov Today. 15:733–740. 2010. View Article : Google Scholar : PubMed/NCBI

8. 

Coiffier B: Diffuse large cell lymphoma. Curr Opin Oncol. 13:325–334. 2001. View Article : Google Scholar : PubMed/NCBI

9. 

Alizadeh AA, Eisen MB, Davis RE, et al: Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature. 403:503–511. 2000. View Article : Google Scholar : PubMed/NCBI

10. 

Hans CP, Weisenburger DD, Greiner TC, et al: Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray. Blood. 103:275–282. 2004. View Article : Google Scholar : PubMed/NCBI

11. 

Davis RE, Brown KD, Siebenlist U, et al: Constitutive nuclear factor kappaB activity is required for survival of activated B cell-like diffuse large B cell lymphoma cells. J Exp Med. 194:1861–1874. 2001. View Article : Google Scholar : PubMed/NCBI

12. 

Thompson RC, Herscovitch M, Zhao I, et al: NF-kappaB down-regulates expression of the B-lymphoma marker CD10 through a miR-155/PU.1 pathway. J Biol Chem. 286:1675–1682. 2011. View Article : Google Scholar : PubMed/NCBI

13. 

Braun T, Carvalho G, Fabre C, et al: Targeting NF-kappaB in hematologic malignancies. Cell Death Differ. 13:748–758. 2006. View Article : Google Scholar : PubMed/NCBI

14. 

Taganov KD, Boldin MP, Chang KJ, et al: NF-kappaB dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Proc Natl Acad Sci USA. 103:12481–12486. 2006. View Article : Google Scholar : PubMed/NCBI

15. 

Li J, Smyth P, Flavin R, et al: Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells. BMC Biotechnol. 7:362007. View Article : Google Scholar : PubMed/NCBI

16. 

Lawrie CH, Soneji S, Marafioti T, et al: MicroRNA expression distinguishes between germinal center B cell-like and activated B cell-like subtypes of diffuse large B cell lymphoma. Int J Cancer. 121:1156–1161. 2007. View Article : Google Scholar

17. 

Nogai H, Dörken B and Lenz GJ: Pathogenesis of non-Hodgkin’s lymphoma. Clin Oncol. 29:1803–1811. 2011.

18. 

Chen C, Ridzon DA, Broomer AJ, et al: Real-time quantificiation of microRNAs by stem-loop RT-PCR. Nucletic Acids Res. 33:e1792005. View Article : Google Scholar : PubMed/NCBI

19. 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI

20. 

Croce CM and Calin GA: miRNAs, cancer, and stem cell division. Cell. 122:6–7. 2005. View Article : Google Scholar : PubMed/NCBI

21. 

Gregory RI and Shiekhattar R: MicroRNA biogenesis and cancer. Cancer Res. 65:3509–3512. 2005. View Article : Google Scholar : PubMed/NCBI

22. 

McManus MT: MicroRNAs and cancer. Semin Cancer Biol. 13:253–258. 2003. View Article : Google Scholar

23. 

Lu J, Getz G, Miska EA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI

24. 

Lawrie CH, Saunders NJ, Soneji S, et al: MicroRNA expression in lymphocyte development and malignancy. Leukemia. 22:1440–1446. 2008. View Article : Google Scholar : PubMed/NCBI

25. 

Lawrie CH, Chi JX, Taylor S, et al: Expression of microRNAs in diffuse large B cell lymphoma is associated with immunophenotype, survival and transformation from follicular lymphoma. J Cell Mol Med. 13:1248–1260. 2009. View Article : Google Scholar : PubMed/NCBI

26. 

Xi Y, Nakajima G, Gavin E, et al: Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples. RNA. 13:1668–1674. 2007. View Article : Google Scholar : PubMed/NCBI

27. 

Georgantas RW III, Hildreth R, Morisot S, et al: CD34+ hematopoietic stem-progenitor cell microRNA expression and function: a circuit diagram of differentiation control. Proc Natl Acad Sci USA. 104:2750–2755. 2007.PubMed/NCBI

28. 

Eis PS, Tam W, Sun L, et al: Accumulation of miR-155 and BIC RNA in human B cell lymphomas. Proc Natl Acad Sci USA. 102:3627–3632. 2005. View Article : Google Scholar : PubMed/NCBI

29. 

Kluiver J, Poppema S, de Jong D, et al: BIC and miR-155 are highly expressed in Hodgkin, primary mediastinal and diffuse large B cell lymphomas. J Pathol. 207:243–249. 2005. View Article : Google Scholar : PubMed/NCBI

30. 

Chang TC, Yu D, Lee YS, et al: Widespread microRNA repression by Myc contributes to tumorigenesis. Nat Genet. 40:43–50. 2008. View Article : Google Scholar : PubMed/NCBI

31. 

Zhang H, Luo XQ, Zhang P, et al: MicroRNA patterns associated with clinical prognostic parameters and CNS relapse prediction in pediatric acute leukemia. PLoS One. 4:e78262009. View Article : Google Scholar : PubMed/NCBI

32. 

Wang Y, Li Z, He C, et al: MicroRNA expression signatures are associated with lineage and survival in acute leukemias. Blood Cells Mol Dis. 44:191–197. 2010. View Article : Google Scholar : PubMed/NCBI

33. 

Visone R, Rassenti LZ, Veronese A, et al: Karyotype-specific microRNA signature in chronic lymphocytic leukemia. Blood. 114:3872–3879. 2009. View Article : Google Scholar : PubMed/NCBI

34. 

Roldo C, Missiaglia E, Hagan JP, et al: MicroRNA expression abnormalities in pancreatic endocrine and acinar tumors are associated with distinctive pathologic features and clinical behavior. J Clin Oncol. 24:4677–4684. 2006. View Article : Google Scholar : PubMed/NCBI

35. 

Volinia S, Galasso M, Costinean S, et al: Reprogramming of miRNA networks in cancer and leukemia. Genome Res. 20:589–599. 2010. View Article : Google Scholar : PubMed/NCBI

36. 

Monticelli S, Ansel KM, Xiao C, et al: MicroRNA profiling of the murine hematopoietic system. Genome Biol. 6:R712005. View Article : Google Scholar : PubMed/NCBI

37. 

Boominathan L: The guardians of the genome (p53, TA-p73, and TA-p63) are regulators of tumor suppressor miRNA network. Cancer Metastasis Rev. 29:613–639. 2010. View Article : Google Scholar : PubMed/NCBI

38. 

Volinia S, Calin GA, Liu CG, et al: A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 103:2257–2261. 2006. View Article : Google Scholar : PubMed/NCBI

39. 

Thai TH, Calado DP, Casola S, et al: Regulation of the germinal center response by microRNA-155. Science. 316:604–608. 2007. View Article : Google Scholar : PubMed/NCBI

40. 

Costinean S, Zanesi N, Pekarsky Y, et al: Pre-B cell proliferation and lymphoblastic leukemia/highgrade lymphoma in E (mu)-miR155 transgenic mice. Proc Natl Acad Sci USA. 103:7024–7029. 2006. View Article : Google Scholar : PubMed/NCBI

41. 

Boldin MP, Taganov KD, Rao DS, et al: miR-146a is a significant brake on autoimmunity, myeloproliferation, and cancer in mice. J Exp Med. 208:1189–1201. 2011. View Article : Google Scholar : PubMed/NCBI

42. 

Lenz G, Wright GW, Emre NCT, et al: Molecular subtypes of diffuse large B-cell lymphoma arise by distinct genetic pathways. Proc Natl Acad Sci USA. 105:13520–13525. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhong H, Xu L, Zhong J, Xiao F, Liu Q, Huang H and Chen F: Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma. Exp Ther Med 3: 763-770, 2012.
APA
Zhong, H., Xu, L., Zhong, J., Xiao, F., Liu, Q., Huang, H., & Chen, F. (2012). Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma. Experimental and Therapeutic Medicine, 3, 763-770. https://doi.org/10.3892/etm.2012.502
MLA
Zhong, H., Xu, L., Zhong, J., Xiao, F., Liu, Q., Huang, H., Chen, F."Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma". Experimental and Therapeutic Medicine 3.5 (2012): 763-770.
Chicago
Zhong, H., Xu, L., Zhong, J., Xiao, F., Liu, Q., Huang, H., Chen, F."Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma". Experimental and Therapeutic Medicine 3, no. 5 (2012): 763-770. https://doi.org/10.3892/etm.2012.502
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong H, Xu L, Zhong J, Xiao F, Liu Q, Huang H and Chen F: Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma. Exp Ther Med 3: 763-770, 2012.
APA
Zhong, H., Xu, L., Zhong, J., Xiao, F., Liu, Q., Huang, H., & Chen, F. (2012). Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma. Experimental and Therapeutic Medicine, 3, 763-770. https://doi.org/10.3892/etm.2012.502
MLA
Zhong, H., Xu, L., Zhong, J., Xiao, F., Liu, Q., Huang, H., Chen, F."Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma". Experimental and Therapeutic Medicine 3.5 (2012): 763-770.
Chicago
Zhong, H., Xu, L., Zhong, J., Xiao, F., Liu, Q., Huang, H., Chen, F."Clinical and prognostic significance of miR-155 and miR-146a expression levels in formalin-fixed/paraffin-embedded tissue of patients with diffuse large B-cell lymphoma". Experimental and Therapeutic Medicine 3, no. 5 (2012): 763-770. https://doi.org/10.3892/etm.2012.502
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