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Article

Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts

  • Authors:
    • Zheng Yuan
    • Wen-Tao Li
    • Xiao-Dan Ye
    • Shi-Yuan Liu
    • Xiang-Sheng Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China, Department of Radiology, Shanghai Chest Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200030, P.R. China, Department of Radiology, Affiliated Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China
  • Pages: 903-907
    |
    Published online on: March 5, 2012
       https://doi.org/10.3892/etm.2012.504
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Abstract

Targeted delivery is a highly desirable strategy for diagnostic imaging due to enhanced efficacy and reduced dosage/toxicity. The need to develop target-specific magnetic resonance imaging (MRI) contrast agents to aid in disease characterization is highly essential. In this study, a specific contrast agent, Gd-DTPA-poly-L-lysine (PL-Gd-DTPA)-folate, was synthesized and evaluated for its efficacy as a targeted agent for the imaging of tumors that overexpress the folate receptor. Folic acid was conjugated to PL-Gd-DTPA via the ε-amino groups. The receptor binding properties of folate-PL-Gd-DTPA were studied in cultured tumor cells that overexpressed the folate receptor. The tumor-selecting properties of folate-PL-Gd-DTPA were then evaluated in BALB/c mice bearing subcutaneously implanted folate receptor-positive tumors. Tissue MR signal intensities were measured at six different time-points. In the in vitro study, the folate-PL-Gd-DTPA was able to bind to these cells, which overexpressed the folate receptor, as with free folic acid. Excellent tumor selectivity was also shown in the animal model; after the success of injection of folate-PL-Gd-DTPA, a maximum intensity increase of 125.4% was observed from pre-injection compared to post‑injection images of the tumor at the 48 h time-point. The liver enhancement was non-specific and the muscle signal intensity at any time-point after injection showed no statistical difference with that observed before injection. Folate-PL-Gd‑DTPA is a promising, novel receptor-specific MRI contrast agent with potential applications in the imaging of human folate receptor-positive tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan Z, Li W, Ye X, Liu S and Xiao X: Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts. Exp Ther Med 3: 903-907, 2012.
APA
Yuan, Z., Li, W., Ye, X., Liu, S., & Xiao, X. (2012). Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts. Experimental and Therapeutic Medicine, 3, 903-907. https://doi.org/10.3892/etm.2012.504
MLA
Yuan, Z., Li, W., Ye, X., Liu, S., Xiao, X."Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts". Experimental and Therapeutic Medicine 3.5 (2012): 903-907.
Chicago
Yuan, Z., Li, W., Ye, X., Liu, S., Xiao, X."Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts". Experimental and Therapeutic Medicine 3, no. 5 (2012): 903-907. https://doi.org/10.3892/etm.2012.504
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan Z, Li W, Ye X, Liu S and Xiao X: Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts. Exp Ther Med 3: 903-907, 2012.
APA
Yuan, Z., Li, W., Ye, X., Liu, S., & Xiao, X. (2012). Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts. Experimental and Therapeutic Medicine, 3, 903-907. https://doi.org/10.3892/etm.2012.504
MLA
Yuan, Z., Li, W., Ye, X., Liu, S., Xiao, X."Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts". Experimental and Therapeutic Medicine 3.5 (2012): 903-907.
Chicago
Yuan, Z., Li, W., Ye, X., Liu, S., Xiao, X."Folate receptor-mediated targeted polymeric gadolinium complexes for magnetic resonance imaging in pulmonary tumor xenografts". Experimental and Therapeutic Medicine 3, no. 5 (2012): 903-907. https://doi.org/10.3892/etm.2012.504
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