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Article

Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma

  • Authors:
    • Chibo Liu
    • Chunqin Pan
    • Yong Liang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Taizhou Municipal Hospital, Taizhou, Zhejiang, P.R. China, Department of Tumorology, Taizhou Municipal Hospital, Taizhou, Zhejiang, P.R. China
  • Pages: 1005-1009
    |
    Published online on: March 14, 2012
       https://doi.org/10.3892/etm.2012.515
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Abstract

The aim of the present study was to screen for possible serum biomarkers for gastric adenocarcinoma. Surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) was used to screen serum samples from 109 cases of gastric adenocarcinoma and 106 control subjects (60 healthy subjects, 30 patients with chronic superficial gastritis and 16 cases of chronic atrophic gastritis). The differentially expressed protein peaks were selected and isolated using high performance liquid chromatography (HPLC) and processed with enzyme prior to liquid chromatography-mass spectrometry tandem mass spectrometry (LC-MS/MS) analysis and data mining with software XCalibur program components BioWorks 3.2. Among the gastric cancer cases, three differentially expressed protein peaks were selected as potential serum biomarkers: the m/z peaks at 5,906.5 showed increased expression (8.53±4.33 in the cancer group, and 0.88±0.31 in the control group); the m/z peaks at 6,635.7 and 8,716.3 showed decreased expression (6.54±2.44 and 0.93±0.29, respectively, in the cancer group and 17.56±4.43 and 2.16±0.98, respectively, in the control group) (P<0.01). The m/z peaks at 5,906.5, 6,635.7 and 8,716.3, were identified as fibrinogen α-chain, apolipoprotein A-II and apolipoprotein C-I. The combined use of the three biomarkers distinguished the cancer group patients from the control group samples at a sensitivity of 93.85% (61/65) and a specificity of 94.34% (50/53). In conclusion, fibrinogen α-chain, apolipoprotein A-II and apolipoprotein C-I were identified as potential markers for gastric cancer and appear to have diagnostic value for clinical applications.
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Copy and paste a formatted citation
Spandidos Publications style
Liu C, Pan C and Liang Y: Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma. Exp Ther Med 3: 1005-1009, 2012.
APA
Liu, C., Pan, C., & Liang, Y. (2012). Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma. Experimental and Therapeutic Medicine, 3, 1005-1009. https://doi.org/10.3892/etm.2012.515
MLA
Liu, C., Pan, C., Liang, Y."Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma". Experimental and Therapeutic Medicine 3.6 (2012): 1005-1009.
Chicago
Liu, C., Pan, C., Liang, Y."Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma". Experimental and Therapeutic Medicine 3, no. 6 (2012): 1005-1009. https://doi.org/10.3892/etm.2012.515
Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Pan C and Liang Y: Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma. Exp Ther Med 3: 1005-1009, 2012.
APA
Liu, C., Pan, C., & Liang, Y. (2012). Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma. Experimental and Therapeutic Medicine, 3, 1005-1009. https://doi.org/10.3892/etm.2012.515
MLA
Liu, C., Pan, C., Liang, Y."Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma". Experimental and Therapeutic Medicine 3.6 (2012): 1005-1009.
Chicago
Liu, C., Pan, C., Liang, Y."Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma". Experimental and Therapeutic Medicine 3, no. 6 (2012): 1005-1009. https://doi.org/10.3892/etm.2012.515
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