Prunella vulgaris aqueous extract attenuates IL-1β-induced apoptosis and NF-κB activation in INS-1 cells

Wu,Huiping; Gao,Ming; Ha,Tuanzhu; Kelley,Jim; Young,Ada; Breuel,Kevin

doi:10.3892/etm.2012.524

Prunella vulgaris aqueous extract attenuates IL-1β-induced apoptosis and NF-κB activation in INS-1 cells

Authors:
- Huiping Wu
- Ming Gao
- Tuanzhu Ha
- Jim Kelley
- Ada Young
- Kevin Breuel
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Affiliations: Department of Biochemistry, Pre-Clinical College, Nanjing University of Chinese Medicine, Nanjing 210029, P.R. China, Department of Surgery, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA, Department of Internal Medicine, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA, Department of Obstetrics and Gynecology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA
Published online on: March 23, 2012 https://doi.org/10.3892/etm.2012.524
Pages: 919-924

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Abstract

We previously reported that Prunella vulgaris aqueous extract (PVAE) promotes hepatic glycogen synthesis and decreases postprandial hyperglycemia in ICR mice. Inflammatory cytokines play a critical role in the pathogenesis of diabetes. This study was designed to examine whether PVAE has a protective effect on IL-1β-induced apoptosis in INS-1 cells. INS-1 pancreatic β cells were plated at 2x106/ml and treated with PVAE (100 µg/ml) 30 min before the cells were challenged with IL-1β (10 ng/ml). Untreated INS-1 cells served as control. INS-1 cell cytotoxicity was examined by MTT and lactate dehydrogenase (LDH) activity assays. Caspase‑3 activity and activation of the apoptotic signaling pathway were analyzed by western blotting. NF‑κB binding activity was examined by EMSA. The levels of inflammatory cytokines in the supernatant were measured by ELISA. IL-1β treatment significantly induced INS-1 cell death by 49.2%, increased LDH activity by 1.5‑fold and caspase‑3 activity by 7.6‑fold, respectively, compared with control cells. However, PVAE administration significantly prevented IL-1β-increased INS-1 cell death and LDH activity and attenuated IL-1β‑increased caspase-3 activity. Western blot data showed that PVAE also significantly attenuated IL-1β-increased Fas, FasL and phospho-JNK levels in the INS-1 cells. In addition, PVAE treatment significantly attenuated IL-1β-increased NF‑κB binding activity and prevented IL‑1β-increased TNF‑α and IL-6 expression in INS-1 cells. Our data suggest that PVAE has a protective effect on IL‑1β-induced INS-1 cell apoptosis. PVAE also attenuates IL‑1β-increased NF‑κB binding activity and inflammatory cytokine expression in INS-1 cells. PVAE may have a benefit for type I diabetic patients.

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