Comparative serum proteomic analysis involving liver organ-specific metastasis-associated proteins of nasopharyngeal carcinoma

Pan,Changchuan; Tao,Yalan; Zhao,Ming; Li,Wang; Huang,Zilin; Gao,Jing; Wu,Yanhen; Yu,Jingrui; Wu,Peihong; Xia,Yunfei; Lu,Jin

doi:10.3892/etm.2012.526

June 2012 Volume 3 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

June 2012 Volume 3 Issue 6

Full Size Image

Article

Comparative serum proteomic analysis involving liver organ-specific metastasis-associated proteins of nasopharyngeal carcinoma

Authors:
- Changchuan Pan
- Yalan Tao
- Ming Zhao
- Wang Li
- Zilin Huang
- Jing Gao
- Yanhen Wu
- Jingrui Yu
- Peihong Wu
- Yunfei Xia
- Jin Lu
View Affiliations / Copyright

Affiliations: Department of Medical Imaging and Interventional Radiology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, Sichuan, P.R. China, Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, Sichuan, P.R. China, The First Affiliated Hospital of Jinan University, Guangzhou, Sichuan, P.R. China, Medical Oncology, Sichuan Cancer Hospital and Institute, The Second People's Hospital of Sichuan Province, Guangzhou, Sichuan, P.R. China
Pages: 1055-1061
|
Published online on: March 23, 2012

https://doi.org/10.3892/etm.2012.526
Expand metrics +

Abstract

Metastasis is the main cause of cancer-related mortality; patients with liver metastases (LM) have the worst prognosis among patients with nasopharyngeal carcinoma (NPC). However, at present, few biomarkers for detecting organ-specific metastasis have been identified. Proteomics, an ultra-sensitive analytical technique, can detect molecular changes before organ-specific metastasis occurs. Analysis with matrix-assisted, laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS), combined with magnetic chemical affinity beads is a new technique for evaluating protein separation. We sought to identify potential liver-specific, metastasis-associated proteomic printing in patients with NPC. We examined 64 serum samples from 50 patients who had pathologically confirmed NPC and 14 who had pathologically confirmed non-NPC with LM using MALDI-TOF-MS with weak cation bead protein chips. During follow-up of at least 37 months (maximum, 176 months) following radiotherapy, we confirmed 16 cases of LM (LM NPC), 16 cases without LM (non-LM NPC) and 18 cases without metastasis (non-M NPC). Using comparison analysis, 4 protein mass peaks, 4155.34, 4194.87, 4210.78 and 4249.56 m/z were identified as liver-specific, metastasis-associated protein peaks in NPC and two of them (4155 and 4249 m/z) met two different statistical criteria in both ClinProt software analyses and discriminant analyses. Models based on the 4 potential serum markers of NPC discriminated between LM NPC, non-LM NPC, non-M NPC and non-NPC LM analyzed with sieved markers. The recognition capability and cross-validation of these models for differentiating the above 4 groups are all approximately 80%. MALDI-TOF-MS combined with tree analysis models may provide a clinical diagnostic platform for detecting potential liver-specific, metastasis-associated proteomic printing in NPC. However, markedly differential proteins still need to be identified.

View Figures

View References

1.	Husemann Y, Geigl JB, Schubert F, et al: Systemic spread is an early step in breast cancer. Cancer Cell. 13:58–68. 2008. View Article : Google Scholar : PubMed/NCBI
2.	Chiang AC and Massague J: Molecular basis of metastasis. N Engl J Med. 359:2814–2823. 2008. View Article : Google Scholar
3.	Kam MK, Teo PM, Chau RM, et al: Treatment of nasopharyngeal carcinoma with intensity-modulated radiotherapy: the Hong Kong experience. Int J Radiat Oncol Biol Phys. 60:1440–1450. 2004. View Article : Google Scholar : PubMed/NCBI
4.	Hui EP, Leung SF, Au JSK, et al: Lung metastasis alone in nasopharyngeal carcinoma: a relatively favorable prognostic group - a study by the Hong Kong nasopharyngeal carcinoma study group. Cancer. 101:300–306. 2004. View Article : Google Scholar : PubMed/NCBI
5.	Teo PM, Kwan WH, Lee WY, et al: Prognosticators determining survival subsequent to distant metastasis from nasopharyngeal carcinoma. Cancer. 77:2423–2431. 1996. View Article : Google Scholar : PubMed/NCBI
6.	Wang CT, Cao KJ, Li Y, et al: Prognosis analysis of nasopharyngeal carcinoma patients with distant metastasis. Ai Zheng. 26:212–215. 2007.PubMed/NCBI
7.	Pan C, He N, Zhao M, et al: Subdividing the M1 stage of liver metastasis for nasopharyngeal carcinoma to better predict meta-static survival. Med Oncol. 28:1349–1355. 2011. View Article : Google Scholar : PubMed/NCBI
8.	Huang CJ, Leung SW, Lian SL, et al: Patterns of distant metastases in nasopharyngeal carcinoma. Kaohsiung J Med Sci. 12:229–234. 1996.PubMed/NCBI
9.	Huang YJ, Xuan C, Zhang BB, et al: SELDI-TOF MS profiling of serum for detection of nasopharyngeal carcinoma. J Exp Clin Cancer Res. 28:852009. View Article : Google Scholar : PubMed/NCBI
10.	Chang KP, Wu CC, Chen HC, et al: Identification of candidate nasopharyngeal carcinoma serum biomarkers by cancer cell secretome and tissue transcriptome analysis: potential usage of cystatin A for predicting nodal stage and poor prognosis. Proteomics. 10:2644–2660. 2010. View Article : Google Scholar
11.	Wu CC, Chien KY, Tsang NM, et al: Cancer cell-secreted proteomes as a basis for searching potential tumor markers: nasopharyngeal carcinoma as a model. Proteomics. 5:3173–3182. 2005. View Article : Google Scholar : PubMed/NCBI
12.	Liao Q, Zhao L, Chen X, et al: Serum proteome analysis for profiling protein markers associated with carcinogenesis and lymph node metastasis in nasopharyngeal carcinoma. Clin Exp Metastasis. 25:465–476. 2008. View Article : Google Scholar : PubMed/NCBI
13.	Cheng AJ, Chen LC, Chien KY, et al: Oral cancer plasma tumor marker identified with bead-based affinity-fractionated proteomic technology. Clin Chem. 51:2236–2244. 2005. View Article : Google Scholar : PubMed/NCBI
14.	Chang JT, Chen LC, Wei SY, et al: Increase diagnostic efficacy by combined use of fingerprint markers in mass spectrometry -plasma peptidomes from nasopharyngeal cancer patients for example. Clin Biochem. 39:1144–1151. 2006. View Article : Google Scholar
15.	Fokas E, Engenhart-Cabillic R, Daniilidis K, et al: Metastasis: the seed and soil theory gains identity. Cancer Metastasis Rev. 26:705–715. 2007. View Article : Google Scholar : PubMed/NCBI
16.	Cho WC: Nasopharyngeal carcinoma: molecular biomarker discovery and progress. Mol Cancer. 6:12007. View Article : Google Scholar : PubMed/NCBI
17.	Hudler P, Gorsic M and Komel R: Proteomic strategies and challenges in tumor metastasis research. Clin Exp Metastasis. 27:441–451. 2010. View Article : Google Scholar : PubMed/NCBI
18.	Liao CC, Mehta A, Ward NJ, et al: Analysis of post-operative changes in serum protein expression profiles from colorectal cancer patients by MALDI-TOF mass spectrometry: a pilot methodological study. World J Surg Oncol. 8:332010. View Article : Google Scholar : PubMed/NCBI
19.	Zheng GX, Wang CX, Qu X, et al: Establishment of serum protein pattern for screening colorectal cancer using SELDITOF-MS. Exp Oncol. 28:282–287. 2006.PubMed/NCBI
20.	Luque-Garcia JL, Martinez-Torrecuadrada JL, Epifano C, et al: Differential protein expression on the cell surface of colorectal cancer cells associated to tumor metastasis. Proteomics. 10:940–952. 2010.PubMed/NCBI
21.	Pang J, Liu WP, Liu XP, et al: Profiling protein markers associated with lymph node metastasis in prostate cancer by DIGE-based proteomics analysis. J Proteome Res. 9:216–226. 2010. View Article : Google Scholar : PubMed/NCBI
22.	Pei H, Zhu H, Zeng S, et al: Proteome analysis and tissue micro-array for profiling protein markers associated with lymph node metastasis in colorectal cancer. J Proteome Res. 6:2495–2501. 2007. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Comparative serum proteomic analysis involving liver organ-specific metastasis-associated proteins of nasopharyngeal carcinoma

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles