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Article

Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells

  • Authors:
    • Wen-Juan Chen
    • Zheng-Ai Xiong
    • Yan Tang
    • Pei-Ting Dong
    • Pan Li
    • Zhi-Gang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China, Institute of Ultrasound Imaging, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China
  • Pages: 999-1004
    |
    Published online on: March 27, 2012
       https://doi.org/10.3892/etm.2012.528
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Abstract

Gene therapy holds great promise for the treatment of diseases. The key problem of gene therapy is the choice of an effective vector. Ultrasound-mediated microbubble technique (UMMT) has already shown promising applications in numerous types of tumors apart from cervical carcinoma. In the present study, according to the results of an MTT assay, we initially chose an ultrasound intensity of 0.5 W/cm2, an ultrasound exposure time of 30 sec and a microbubble concentration of 10% as the optimum experimental condition for wtp53 plasmid transfection into HeLa cells. To further investigate the transfection efficiency of ultrasound combined with microbubbles, RT-PCR analysis was used to examine the mRNA level of p53. The transfection efficiency in the plasmid plus microbubbles and ultrasound group was significantly higher than that of the other groups. Following transfection of the wtp53 gene, flow cytometric analysis showed that the cell cycle of HeLa cells was arrested in the G1 phase. The results of the present study suggest that UMMT, a new gene delivery system, increases the transfection efficiency of the wtp53 gene. Moreover, the growth of HeLa cells was arrested by introducing wtp53. This study may afford a new trend for the gene therapy of cervical carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Chen W, Xiong Z, Tang Y, Dong P, Li P and Wang Z: Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells. Exp Ther Med 3: 999-1004, 2012.
APA
Chen, W., Xiong, Z., Tang, Y., Dong, P., Li, P., & Wang, Z. (2012). Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells. Experimental and Therapeutic Medicine, 3, 999-1004. https://doi.org/10.3892/etm.2012.528
MLA
Chen, W., Xiong, Z., Tang, Y., Dong, P., Li, P., Wang, Z."Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells". Experimental and Therapeutic Medicine 3.6 (2012): 999-1004.
Chicago
Chen, W., Xiong, Z., Tang, Y., Dong, P., Li, P., Wang, Z."Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells". Experimental and Therapeutic Medicine 3, no. 6 (2012): 999-1004. https://doi.org/10.3892/etm.2012.528
Copy and paste a formatted citation
x
Spandidos Publications style
Chen W, Xiong Z, Tang Y, Dong P, Li P and Wang Z: Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells. Exp Ther Med 3: 999-1004, 2012.
APA
Chen, W., Xiong, Z., Tang, Y., Dong, P., Li, P., & Wang, Z. (2012). Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells. Experimental and Therapeutic Medicine, 3, 999-1004. https://doi.org/10.3892/etm.2012.528
MLA
Chen, W., Xiong, Z., Tang, Y., Dong, P., Li, P., Wang, Z."Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells". Experimental and Therapeutic Medicine 3.6 (2012): 999-1004.
Chicago
Chen, W., Xiong, Z., Tang, Y., Dong, P., Li, P., Wang, Z."Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells". Experimental and Therapeutic Medicine 3, no. 6 (2012): 999-1004. https://doi.org/10.3892/etm.2012.528
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