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Article

Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats

  • Authors:
    • Turgut Teke
    • Emin Maden
    • Aysel Kiyici
    • Celalettin Korkmaz
    • Mehmet Gok
    • Faruk Ozer
    • Oktay Imecik
    • Kursat Uzun
  • View Affiliations / Copyright

    Affiliations: Department of Chest Diseases, Meram Medical Faculty, Konya University, 42080 Meram-Konya, Turkey, Department of Biochemistry, Meram Medical Faculty, Konya University, 42080 Meram-Konya, Turkey
  • Pages: 121-124
    |
    Published online on: April 17, 2012
       https://doi.org/10.3892/etm.2012.550
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Abstract

Bleomycin causes pulmonary fibrosis by increasing free oxygen radicals. Cigarette smoke is a strong oxidant which adversely affects pulmonary tissue. We evaluated the effects of cigarette smoke administered with intratracheal bleomycin on pulmonary tissue. We studied 3 groups of rats (n=10): one group received intratracheal saline and served as a control; one received intratracheal bleomycin (IT) (0.5 U/100 g body weight, single dose on the first day), and one group received intratracheal bleomycin (single dose on first day) and tobacco smoke (two times per day) (IT-S). After 4 weeks, the levels of malondialdehyde (MDA) and nitric oxide (NO) and the activities of superoxide dismutase (SOD) and xanthine oxidase (XO) were assayed in the homogenate of the lung tissue samples. The severity of interstitial fibrosis was assessed using the grading system described by Ashcroft. There was more intensive fibrosis in the IT and IT-S than in the control samples (P<0.001). The levels of MDA, NO and activity of XO were significantly increased (P<0.001, <0.002 and <0.002, respectively), and SOD activity (P<0.001) was significantly decreased in the IT group when compared to these values in the control group. The concentration of NO was significantly decreased (P<0.002), and SOD activity was significantly increased (P<0.05) in the lung tissue samples of the IT-S group. Theoretically, the combination of cigarette smoke and bleomycin may have a synergistic effect on oxidative lung injury. In conclusion, we showed that inhalation of cigarette smoke provides protection against oxidative stress in the lung tissue of rats with bleomycin-induced pulmonary fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Teke T, Maden E, Kiyici A, Korkmaz C, Gok M, Ozer F, Imecik O and Uzun K: Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats. Exp Ther Med 4: 121-124, 2012.
APA
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F. ... Uzun, K. (2012). Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats. Experimental and Therapeutic Medicine, 4, 121-124. https://doi.org/10.3892/etm.2012.550
MLA
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F., Imecik, O., Uzun, K."Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats". Experimental and Therapeutic Medicine 4.1 (2012): 121-124.
Chicago
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F., Imecik, O., Uzun, K."Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats". Experimental and Therapeutic Medicine 4, no. 1 (2012): 121-124. https://doi.org/10.3892/etm.2012.550
Copy and paste a formatted citation
x
Spandidos Publications style
Teke T, Maden E, Kiyici A, Korkmaz C, Gok M, Ozer F, Imecik O and Uzun K: Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats. Exp Ther Med 4: 121-124, 2012.
APA
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F. ... Uzun, K. (2012). Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats. Experimental and Therapeutic Medicine, 4, 121-124. https://doi.org/10.3892/etm.2012.550
MLA
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F., Imecik, O., Uzun, K."Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats". Experimental and Therapeutic Medicine 4.1 (2012): 121-124.
Chicago
Teke, T., Maden, E., Kiyici, A., Korkmaz, C., Gok, M., Ozer, F., Imecik, O., Uzun, K."Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats". Experimental and Therapeutic Medicine 4, no. 1 (2012): 121-124. https://doi.org/10.3892/etm.2012.550
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