Anti-IL-6 receptor antibody does not ameliorate radiation pneumonia in mice

Ogata,Toshiyuki; Yamazaki,Hideya; Teshima,Teruki; Tsuchiya,Takahiro; Nishimoto,Norihiro; Matsuura,Nariaki

doi:10.3892/etm.2012.582

Anti-IL-6 receptor antibody does not ameliorate radiation pneumonia in mice

Authors:
- Toshiyuki Ogata
- Hideya Yamazaki
- Teruki Teshima
- Takahiro Tsuchiya
- Norihiro Nishimoto
- Nariaki Matsuura
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Affiliations: Department of Radiation Oncology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Radiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-856, Japan, Department of Medical Physics and Engineering, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Laboratory of Immune Regulation, Wakayama Medical University, Ibaraki, Osaka 567-0085, Japan, Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan
Published online on: May 18, 2012 https://doi.org/10.3892/etm.2012.582
Pages: 273-276

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Abstract

We previously showed that early administration of monoclonal anti-interleukin-6 receptor antibody (IL-6RA) does not prevent radiation-induced lung injury in mice. The purpose of this study was to investigate whether a higher dose and longer course of IL-6RA treatment was effective in ameliorating radiation pneumonia. C57Bl/6J mice received thoracic irradiation of 12 Gy, and were intraperitoneally injected with the IL-6RA, namely MR16-1, or with control rat IgG 4 times, once immediately following exposure and then weekly from 1 to 3 weeks after irradiation. Enzyme-linked immunosorbent assays were used to analyze the plasma levels of IL-6 and serum amyloid A (SAA). Lung injury was assessed by histological staining with haematoxylin and eosin (H&E) and by measuring wet lung weight. We observed marked upregulation of IL-6 in IL-6RA-treated mice compared to the IgG-treated control group, whereas IL-6RA did not increase the production of SAA in the group receiving irradiation. However, radiation pneumonia, as evaluated by H&E staining and lung weight showed no differences between the IL-6RA-treated mice and the controls. Long-term treatment with high-dose IL-6RA does not ameliorate radiation pneumonia.

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