Co-transfection of dendritic cells with AFP and IL-2 genes enhances the induction of tumor antigen-specific antitumor immunity

  • Authors:
    • Jing-Yue Yang
    • Xiao Li
    • Li Gao
    • Zeng-Hui Teng
    • Wen-Chao Liu
  • View Affiliations

  • Published online on: July 6, 2012     https://doi.org/10.3892/etm.2012.635
  • Pages: 655-660
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Abstract

Dendritic cells (DCs) are highly efficient, specialized antigen-presenting cells and DCs transfected with tumor-related antigens are regarded as promising vaccines in cancer immunotherapy. The aim of the present study was to investigate whether DCs co-transfected with the α-fetoprotein (AFP) and human interleukin-2 (IL-2) genes were able to induce stronger therapeutic antitumor immunity in transfected DCs. In this study, DCs from hepatocellular carcinoma (HCC) patients were co-transfected with the IL-2 gene and/or the AFP gene. The reverse transcription-PCR (RT-PCR) data revealed that the DCs transfected with the adenovirus AdAFP/IL-2 expressed AFP and IL-2. The DCs co-transfected with IL-2 and AFP (AFP/IL-2-DCs) enhanced the cytotoxicities of cytotoxic T lymphocytes (CTLs) and increased the production of IL-2 and interferon-γ significantly compared with their AFP-DC, green fluorescent protein (GFP)-DC, DC or phosphate‑buffered saline (PBS) counterparts. In vivo data suggested that immunization with AFP-DCs enhances antigen-specific antitumor efficacy more potently than immunization with IL-2-DCs or AFP-DCs. These findings provide a potential strategy to improve the efficacy of DC-based tumor vaccines.
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October 2012
Volume 4 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yang J, Li X, Gao L, Teng Z and Liu W: Co-transfection of dendritic cells with AFP and IL-2 genes enhances the induction of tumor antigen-specific antitumor immunity. Exp Ther Med 4: 655-660, 2012
APA
Yang, J., Li, X., Gao, L., Teng, Z., & Liu, W. (2012). Co-transfection of dendritic cells with AFP and IL-2 genes enhances the induction of tumor antigen-specific antitumor immunity. Experimental and Therapeutic Medicine, 4, 655-660. https://doi.org/10.3892/etm.2012.635
MLA
Yang, J., Li, X., Gao, L., Teng, Z., Liu, W."Co-transfection of dendritic cells with AFP and IL-2 genes enhances the induction of tumor antigen-specific antitumor immunity". Experimental and Therapeutic Medicine 4.4 (2012): 655-660.
Chicago
Yang, J., Li, X., Gao, L., Teng, Z., Liu, W."Co-transfection of dendritic cells with AFP and IL-2 genes enhances the induction of tumor antigen-specific antitumor immunity". Experimental and Therapeutic Medicine 4, no. 4 (2012): 655-660. https://doi.org/10.3892/etm.2012.635