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Article

Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29

  • Authors:
    • Ting Liu
    • Dan Liu
    • Jing Liu
    • Ji-Tao Song
    • Shan-Ling Gao
    • Hui Li
    • Li-Hong Hu
    • Bing-Rong Liu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
  • Pages: 716-722
    |
    Published online on: July 24, 2012
       https://doi.org/10.3892/etm.2012.647
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Abstract

This study aimed to investigate the impact of the combined use of the nuclear factor-κB (NF-κB) inhibitors pyrrolidine dithiocarbamate (PDTC), bortezomib or SN50, and the chemotherapy agents arsenic acid (As2O3), fluorouracil (5FU), oxaliplatin or paclitaxel on the growth and apoptosis of HT-29 cells. Cell morphology was observed using inverted microscopy, and cell viability and apoptosis were assessed using the MTT assay and flow cytometry, respectively. The activities of NF-κB were analyzed by western blotting and electrophoretic mobility shift assay (EMSA). Cell growth was significantly inhibited by As2O3, oxaliplatin and paclitaxel in a time- and concentration-dependent manner (P<0.05), while 5FU inhibited cell growth in a time-dependent manner only (P<0.05). The growth inhibition rate and apoptosis induction ratio were increased following the combined treatment of the chemotherapy agent and NF-κB inhibitor. The expression of NF-κB p65 was upregulated when cells were treated with a chemotherapy drug, however it was downregulated following combined treatment or treatment with an NF-κB inhibitor alone. In conclusion, an NF-κB inhibitor combined with a chemotherapy drug effectively inhibited cell proliferation, induced cell apoptosis and inhibited NF-κB activity to enhance the chemotherapeutic sensitivity of HT-29 cells.
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1 

Sen R and Baltimore D: Multiple nuclear factors interact with the immunoglobulin enhancer sequences. Cell. 46:705–716. 1986. View Article : Google Scholar : PubMed/NCBI

2 

Zandi E and Karin M: Bridging the gap: composition, regulation, and physiological function of the IkappaB kinase complex. Mol Cell Biol. 19:4547–4551. 1999.PubMed/NCBI

3 

Baldwin AS: Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB. J Clin Invest. 3:241–246. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Gottesman MM, Fojo T and Bates SE: Multidrug resistance in cancer: role of ATP-dependent transporters. Nat Rev Cancer. 2:48–58. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Wang CY, Guttridge DC, Mayo MW and Baldwin AS Jr: NF-kappaB induces expression of the Bcl-2 homologue A1/Bfl-1 to preferentially suppress chemotherapy-induced apoptosis. Mol Cell Biol. 19:5923–5929. 1999.PubMed/NCBI

6 

Wang CY, Mayo MW, Korneluk RG, Goeddel DV and Baldwin AS Jr: NF-kappaB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation. Science. 281:1680–1683. 1998. View Article : Google Scholar : PubMed/NCBI

7 

Karin M: Nuclear factor-kappaB in cancer development and progression. Nature. 441:431–436. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Mitsiades N, Mitsiades CS, Poulaki V, Anderson KC and Treon SP: Intracellular regulation of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in human multiple myeloma cells. Blood. 99:2162–2171. 2002. View Article : Google Scholar

9 

Poynter JN, Haile RW, Siegmund KD, Campbell PT, Figueiredo JC, Limburg P, Young J, Le Marchand L, Potter JD, Cotterchio M, Casey G, Hopper JL, Jenkins MA, Thibodeau SN, Newcomb PA and Baron JA; Colon Cancer Family Registry: Associations between smoking, alcohol consumption, and colorectal cancer, overall and by tumor microsatellite instability status. Cancer Epidemiol Biomarkers Prev. 18:2745–2750. 2009. View Article : Google Scholar

10 

Bostick RM, Potter JD, Kushi LH, Sellers TA, Steinmetz KA, McKenzie DR, Gapstur SM and Folsom AR: Sugar, meat and fat intake, and non-dietary risk factors for colon cancer incidence in Iowa women. Cancer Causes Control. 5:38–52. 1994. View Article : Google Scholar : PubMed/NCBI

11 

Chan AT and Giovannucci EL: Primary prevention of colorectal cancer. Gastroenterology. 138:2029–2043. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Kim HJ, Hawke N and Baldwin AS: NF-kappaB and IKK as therapeutic targets in cancer. Cell Death Differ. 13:738–747. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Legrand-Poels S, Schoonbroodt S, Matroule JY and Piette J: NF-kappaB: an important transcription factor in photobiology. Photochem Photobiol B. 45:1–8. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Romano MF, Lamberti A, Bisogni R, Tassone P, Pagnini D, Storti G, Del Vecchio L, Turco MC and Venuta S: Enhancement of cytosine arabinoside induced apoptosis in human myeloblastic leukemia cells by NF kappaB/Rel-specific decoy oligodeoxy-nucleotides. Gene Ther. 7:1234–1237. 2000. View Article : Google Scholar

15 

Beg AA, Sha WC, Bronson RT, Ghosh S and Baltimore D: Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-kappaB. Nature. 376:167–170. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Holmes-McNary M and Baldwin AS Jr: Chemopreventive properties of trans-resveratrol are associated with inhibition of activation of IkappaB kinase. Cancer Res. 60:3477–3483. 2000.PubMed/NCBI

17 

Yemelyanov A, Gasparian A, Lindholm P, Dang L, Pierce JW, Kisseljov F, Karseladze A and Budunova I: Effects of IKK inhibitor PS1145 on NF-kappaB function, proliferation, apoptosis and invasion activity in prostate carcinoma cells. Oncogene. 25:387–398. 2006.PubMed/NCBI

18 

Wu LF, Li GP, Su JD, Pu ZJ, Feng JL, Ye YQ and Wei BL: Involvement of NF-kappaB activation in the apoptosis induced by extracellular adenosine in human hepatocellular carcinoma HepG2 cells. Biochem Cell Biol. 88:705–714. 2010.PubMed/NCBI

19 

Furuta H, Osawa K, Shin M, Ishikawa A, Matsuo K, Khan M, Aoki K, Ohya K, Okamoto M, Tominaga K, et al: Selective inhibition of NF-κB suppresses bone invasion by oral squamous cell carcinoma in vivo. Int J Cancer. Jan 19–2012.(Epub ahead of print).

20 

Zhu BS, Xing CG, Lin F, Fan XQ, Zhao K and Qin ZH: Blocking NF-κB nuclear translocation leads to p53-related autophagy activation and cell apoptosis. World J Gastroenterol. 17:478–487. 2011.

21 

Si X, McManus BM, Zhang JC, Yuan J, Cheung C, Esfandiarei M, Suarez A, Morgan A and Luo HL: Pyrrolidine dithiocarbamate reduces coxsackievirus B3 replication through inhibition of the ubiquitin-proteasome pathway. J Virol. 79:8014–8023. 2005. View Article : Google Scholar

22 

Caravita T, de Fabritiis P, Palumbo A, Amadori S and Boccadoro M: Bortezomib: efficacy comparisons in solid tumors and hematologic malignancies. Nat Clin Pract Oncol. 3:374–387. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Nencioni A, Grünebach F, Patrone F, Ballestrero A and Brossart P: Proteasome inhibitors: antitumor effects and beyond. Leukemia. 21:30–36. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Orange JS and May MJ: Cell penetrating peptide inhibitors of nuclear factor-kappaB. Cell Mol Life Sci. 65:3564–3591. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu T, Liu D, Liu J, Song J, Gao S, Li H, Hu L and Liu B: Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29. Exp Ther Med 4: 716-722, 2012.
APA
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H. ... Liu, B. (2012). Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29. Experimental and Therapeutic Medicine, 4, 716-722. https://doi.org/10.3892/etm.2012.647
MLA
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H., Hu, L., Liu, B."Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29". Experimental and Therapeutic Medicine 4.4 (2012): 716-722.
Chicago
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H., Hu, L., Liu, B."Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29". Experimental and Therapeutic Medicine 4, no. 4 (2012): 716-722. https://doi.org/10.3892/etm.2012.647
Copy and paste a formatted citation
x
Spandidos Publications style
Liu T, Liu D, Liu J, Song J, Gao S, Li H, Hu L and Liu B: Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29. Exp Ther Med 4: 716-722, 2012.
APA
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H. ... Liu, B. (2012). Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29. Experimental and Therapeutic Medicine, 4, 716-722. https://doi.org/10.3892/etm.2012.647
MLA
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H., Hu, L., Liu, B."Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29". Experimental and Therapeutic Medicine 4.4 (2012): 716-722.
Chicago
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H., Hu, L., Liu, B."Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29". Experimental and Therapeutic Medicine 4, no. 4 (2012): 716-722. https://doi.org/10.3892/etm.2012.647
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