Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29

  • Authors:
    • Ting Liu
    • Dan Liu
    • Jing Liu
    • Ji-Tao Song
    • Shan-Ling Gao
    • Hui Li
    • Li-Hong Hu
    • Bing-Rong Liu
  • View Affiliations

  • Published online on: July 24, 2012     https://doi.org/10.3892/etm.2012.647
  • Pages: 716-722
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Abstract

This study aimed to investigate the impact of the combined use of the nuclear factor-κB (NF-κB) inhibitors pyrrolidine dithiocarbamate (PDTC), bortezomib or SN50, and the chemotherapy agents arsenic acid (As2O3), fluorouracil (5FU), oxaliplatin or paclitaxel on the growth and apoptosis of HT-29 cells. Cell morphology was observed using inverted microscopy, and cell viability and apoptosis were assessed using the MTT assay and flow cytometry, respectively. The activities of NF-κB were analyzed by western blotting and electrophoretic mobility shift assay (EMSA). Cell growth was significantly inhibited by As2O3, oxaliplatin and paclitaxel in a time- and concentration-dependent manner (P<0.05), while 5FU inhibited cell growth in a time-dependent manner only (P<0.05). The growth inhibition rate and apoptosis induction ratio were increased following the combined treatment of the chemotherapy agent and NF-κB inhibitor. The expression of NF-κB p65 was upregulated when cells were treated with a chemotherapy drug, however it was downregulated following combined treatment or treatment with an NF-κB inhibitor alone. In conclusion, an NF-κB inhibitor combined with a chemotherapy drug effectively inhibited cell proliferation, induced cell apoptosis and inhibited NF-κB activity to enhance the chemotherapeutic sensitivity of HT-29 cells.
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October 2012
Volume 4 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu T, Liu D, Liu J, Song J, Gao S, Li H, Hu L and Liu B: Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29. Exp Ther Med 4: 716-722, 2012
APA
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H. ... Liu, B. (2012). Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29. Experimental and Therapeutic Medicine, 4, 716-722. https://doi.org/10.3892/etm.2012.647
MLA
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H., Hu, L., Liu, B."Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29". Experimental and Therapeutic Medicine 4.4 (2012): 716-722.
Chicago
Liu, T., Liu, D., Liu, J., Song, J., Gao, S., Li, H., Hu, L., Liu, B."Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29". Experimental and Therapeutic Medicine 4, no. 4 (2012): 716-722. https://doi.org/10.3892/etm.2012.647