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Print ISSN: 1792-0981 Online ISSN: 1792-1015
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October 2012 Volume 4 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice

  • Authors:
    • Zhen-Hai Ma
    • Jin-Hui Ma
    • Li Jia
    • Yong-Fu Zhao
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, P.R. China, College of Laboratory Medicine, Dalian Medical University, Dalian 116044, P.R. China
  • Pages: 621-626
    |
    Published online on: August 1, 2012
       https://doi.org/10.3892/etm.2012.652
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Abstract

The present study aimed to investigate the influence of COL8A1 expression on cell invasiveness, drug sensitivity and tumorigenicity of hepatocellular carcinoma Hepa1-6 cells with low metastatic potential. COL8A1-1-pEGFP-N2 and pEGFP-N2 were transfected into experimental and control group cells. The COL8A1 expression in transfected Hepa1-6 cells was analyzed with RT-PCR and western blot analysis. The invasive potential of transfected Hepa1-6 cells was tested in invasion experiments in vitro and the tumorigenic ability of the transfected Hepa1-6 cells was tested in mouse tumors in vivo. Hepa1-6 cell proliferation and D-limonene sensitivity was analyzed using the MTT method. Expression of COL8A1 in the Hepa1-6/COL8A1 group showed a significant increase when compared with the untransfected cells of the Hepa1-6 control group and empty‑plasmid transfected cells from the Hepa1-6/mock control group. Enhanced COL8A1 expression increased cell proliferation and matrix adhesion ability via invasion and tumorigenesis in vivo while the sensitivity to D-limonene was concurrently inhibited. The expression of COL8A1 in hepatocarcinoma cells was correlated with increased tumor cell proliferation, invasion, in vivo tumorigenicity and reduced antitumor drug sensitivity, and may provide novel targets for tumor therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Z, Ma J, Jia L and Zhao Y: Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Exp Ther Med 4: 621-626, 2012.
APA
Ma, Z., Ma, J., Jia, L., & Zhao, Y. (2012). Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Experimental and Therapeutic Medicine, 4, 621-626. https://doi.org/10.3892/etm.2012.652
MLA
Ma, Z., Ma, J., Jia, L., Zhao, Y."Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice". Experimental and Therapeutic Medicine 4.4 (2012): 621-626.
Chicago
Ma, Z., Ma, J., Jia, L., Zhao, Y."Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice". Experimental and Therapeutic Medicine 4, no. 4 (2012): 621-626. https://doi.org/10.3892/etm.2012.652
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Z, Ma J, Jia L and Zhao Y: Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Exp Ther Med 4: 621-626, 2012.
APA
Ma, Z., Ma, J., Jia, L., & Zhao, Y. (2012). Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Experimental and Therapeutic Medicine, 4, 621-626. https://doi.org/10.3892/etm.2012.652
MLA
Ma, Z., Ma, J., Jia, L., Zhao, Y."Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice". Experimental and Therapeutic Medicine 4.4 (2012): 621-626.
Chicago
Ma, Z., Ma, J., Jia, L., Zhao, Y."Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice". Experimental and Therapeutic Medicine 4, no. 4 (2012): 621-626. https://doi.org/10.3892/etm.2012.652
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