The membrane-cytoskeletal protein 4.1N is involved in the process of cell adhesion, migration and invasion of breast cancer cells

Ji,Zhenyu; Shi,Xiaofang; Liu,Xin; Shi,Yu; Zhou,Qingqing; Liu,Xilong; Li,Li; Ji,Xiang; Gao,Yanfeng; Qi,Yuanming; Kang,Qiaozhen

doi:10.3892/etm.2012.653

The membrane-cytoskeletal protein 4.1N is involved in the process of cell adhesion, migration and invasion of breast cancer cells

Authors:
- Zhenyu Ji
- Xiaofang Shi
- Xin Liu
- Yu Shi
- Qingqing Zhou
- Xilong Liu
- Li Li
- Xiang Ji
- Yanfeng Gao
- Yuanming Qi
- Qiaozhen Kang
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Affiliations: Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, P.R. China, Basic Medical College of Zhengzhou University, Zhengzhou 450001, P.R. China
Published online on: August 3, 2012 https://doi.org/10.3892/etm.2012.653
Pages: 736-740

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Abstract

Protein 4.1N belongs to the protein 4.1 superfamily that links transmembrane proteins to the actin cytoskeleton. Recent evidence has shown that protein 4.1 is important in tumor suppression. However, the functions of 4.1N in the metastasis of breast cancer are largely unknown. In the present study, MCF-7, T-47D and MDA-MB-231 breast cancer cell lines with various metastatic abilities were employed. Protein 4.1N was found to be expressed in poorly metastatic MCF-7 and middle metastatic T-47D cell lines, and was predominantly associated with cell-cell junctions. However, no 4.1N expression was detected in the highly metastatic MDA-MB‑231 cells. Moreover, re-expression of 4.1N in MDA-MB-231 cells inhibited cell adhesion, migration and invasion. The results suggest that protein 4.1N is a negative regulator of cell metastasis in breast cancer.

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