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November 2012 Volume 4 Issue 5

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome

  • Authors:
    • Xiaojuan Yin
    • Lihua Li
    • Hanxiao Fan
    • Wenwen Qu
    • Lu Xie
    • Zhichun Feng
  • View Affiliations / Copyright

    Affiliations: Affiliated Bayi Children's Hospital, Beijing Military Region General Hospital, No. 5 Nan Mencang, Dongcheng District, Beijing 100700, P.R. China
  • Pages: 815-819
    |
    Published online on: August 17, 2012
       https://doi.org/10.3892/etm.2012.673
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Abstract

The aim of this study was to investigate whether surfactant-associated protein B (SP-B) mRNA deficiency is involved in the pathogenesis of neonatal respiratory distress syndrome (RDS). A total of 60 unrelated neonates who died of RDS were recruited as the RDS group and subgrouped into a ≤32-, a 32-36+6- and a ≥37-week group (n=20 per group) on the basis of gestational age. In addition, 60 neonates who succumbed to other diseases were enrolled as controls. The lung tissues were collected within 30 min after death. In situ hybridization was conducted to detect SP-B mRNA expression in the lung. The frequency of SP-B mRNA deficiency was also calculated. Among the RDS groups, the SP-B mRNA levels were significantly higher compared to those in the control group (t=7.812, P<0.001), but were comparable among RDS patients with different gestational ages (F=2.348, P>0.105). Among the control groups, the SP-B mRNA levels increased with the increase in gestational age (F=50.124, P<0.001). In the ≤32-week group, the number of cells positive for SP-B mRNA in RDS patients was markedly reduced as compared to that of the controls (t=3.185, P<0.01). In the 32-36+6-week group, the number of cells positive for SP-B mRNA in RDS patients was significantly smaller compared to that of the controls (t=9.342, P<0.001). In the ≥37-week group, the number of cells positive for SP-B mRNA in RDS patients was markedly smaller compared to that in the controls (t=4.238, P<0.001). Among RDS neonates, SP-B mRNA deficiency was noted in 35 patients with a frequency of 58.3%. In the control group, SP-B mRNA deficiency was noted in 8 patients with a frequency of 13.3%, which was markedly lower compared to that in the RDS group (χ2=26.421, P<0.001). The results of the present study therefore suggest that SP-B mRNA deficiency is involved in the pathogenesis of RDS.
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Copy and paste a formatted citation
Spandidos Publications style
Yin X, Li L, Fan H, Qu W, Xie L and Feng Z: Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome. Exp Ther Med 4: 815-819, 2012.
APA
Yin, X., Li, L., Fan, H., Qu, W., Xie, L., & Feng, Z. (2012). Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome. Experimental and Therapeutic Medicine, 4, 815-819. https://doi.org/10.3892/etm.2012.673
MLA
Yin, X., Li, L., Fan, H., Qu, W., Xie, L., Feng, Z."Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome". Experimental and Therapeutic Medicine 4.5 (2012): 815-819.
Chicago
Yin, X., Li, L., Fan, H., Qu, W., Xie, L., Feng, Z."Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome". Experimental and Therapeutic Medicine 4, no. 5 (2012): 815-819. https://doi.org/10.3892/etm.2012.673
Copy and paste a formatted citation
x
Spandidos Publications style
Yin X, Li L, Fan H, Qu W, Xie L and Feng Z: Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome. Exp Ther Med 4: 815-819, 2012.
APA
Yin, X., Li, L., Fan, H., Qu, W., Xie, L., & Feng, Z. (2012). Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome. Experimental and Therapeutic Medicine, 4, 815-819. https://doi.org/10.3892/etm.2012.673
MLA
Yin, X., Li, L., Fan, H., Qu, W., Xie, L., Feng, Z."Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome". Experimental and Therapeutic Medicine 4.5 (2012): 815-819.
Chicago
Yin, X., Li, L., Fan, H., Qu, W., Xie, L., Feng, Z."Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome". Experimental and Therapeutic Medicine 4, no. 5 (2012): 815-819. https://doi.org/10.3892/etm.2012.673
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