Stress increases periodontal inflammation

Rivera,César; Monsalve,Francisco; Suazo,Iván; Becerra,Javiera

doi:10.3892/etm.2012.675

Stress increases periodontal inflammation

Authors:
- César Rivera
- Francisco Monsalve
- Iván Suazo
- Javiera Becerra
View Affiliations

Affiliations: Unit of Histology and Embryology, Department of Basic Biomedical Sciences, Faculty of Health Sciences, University of Talca, Talca, Chile, Faculty of Medicine, Diego Portales University, Santiago, Chile, School of Dentistry, Faculty of Health Sciences, University of Talca, Talca, Chile
Published online on: August 20, 2012 https://doi.org/10.3892/etm.2012.675
Pages: 883-888

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

This study aimed to examine the effect of chronic restraint stress (RS) on the severity of experimental periodontal disease in rats. A total of 32 male Sprague Dawley (SD) rats were divided into four groups: i) Rats receiving two treatment regimens, chronic stress induced by movement restriction in acrylic cylinders for 1-1.5 h daily and induction of experimental periodontal disease, using a nylon ligature which was placed around the first left mandibular molars (n=8); ii) induction of periodontal disease, without RS (n=8); iii) RS (n=8) and iv) control (n=8). After 15 days, blood samples were obtained, and blood glucose levels and the corticosterone concentration were measured as stress markers. The severity of periodontal disease was analyzed according to the level of gingival and bone inflammation, leading to compromise of the teeth involved. Chronic stress was induced with movement restriction (P≤0.05, Mann-Whitney U-test) and increased the severity (P≤0.05, Mann-Whitney U-test) of experimental periodontal disease in rats, according to the level of gingival and bone inflammation around the first left mandibular molars. The results of the present study showed that RS modulates periodontal inflammation and that the rat model described herein is suitable for investigating the association between stress and periodontal disease.

View Figures

View References

1	Gaspersic R, Stiblar-Martincic D and Skaleric U: Influence of restraint stress on ligature-induced periodontitis in rat. Eur J Oral Sci. 110:125–129. 2002. View Article : Google Scholar : PubMed/NCBI
2	Takada T, Yoshinari N, Sugiishi S, Kawase H, Yamane T and Noguchi T: Effect of restraint stress on the progression of experimental periodontitis in rats. J Periodontol. 75:306–315. 2004. View Article : Google Scholar : PubMed/NCBI
3	Peruzzo DC, Benatti BB, Antunes IB, Andersen ML, Sallum EA, Casati MZ, Nociti FH Jr and Nogueira-Filho GR: Chronic stress may modulate periodontal disease: a study in rats. J Periodontol. 79:697–704. 2008. View Article : Google Scholar : PubMed/NCBI
4	Oz H and Puleo D: Animal models for periodontal disease. J Biomed Biotechnol. 7548572011.PubMed/NCBI
5	Genco RJ, Ho AW, Grossi SG, Dunford RG and Tedesco LA: Relationship of stress, distress, and inadequate coping behaviors to periodontal disease. J Periodontol. 70:711–723. 1999. View Article : Google Scholar : PubMed/NCBI
6	Hildebrand HC, Epstein J and Larjava H: The influence of psychological stress on periodontal disease. J West Soc Periodontol Periodontal Abstr. 48:69–77. 2000.PubMed/NCBI
7	Green LW, Tryon WW, Marks B and Huryn J: Periodontal disease as a function of life events stress. J Hum Stress. 12:32–36. 1986. View Article : Google Scholar : PubMed/NCBI
8	Freeman R and Gross S: Stress measures as predictors of periodontal disease-a preliminary communication. Community Dent Oral Epidemiol. 21:176–177. 1993. View Article : Google Scholar : PubMed/NCBI
9	Wimmer G, Janda M, Wieselmann-Penkner K, Jakse N, Polansky R and Pertl C: Coping with stress: its influence on periodontal disease. J Periodontol. 73:1343–1351. 2002. View Article : Google Scholar : PubMed/NCBI
10	Hilgert JB, Hugo FN, Bandeira DR and Bozzetti MC: Stress, cortisol, and periodontitis in a population aged 50 years and over. J Dent Res. 85:324–328. 2006.PubMed/NCBI
11	Pistorius A, Krahwinkel T, Willershausen B and Boekstegen C: Relationship between stress factors and periodontal disease. Eur J Med Res. 7:393–398. 2002.PubMed/NCBI
12	Breivik T, Thrane PS, Murison R and Gjermo P: Emotional stress effects on immunity, gingivitis and periodontitis. Eur J Oral Sci. 104:327–334. 2007. View Article : Google Scholar : PubMed/NCBI
13	Paré WP and Glavin GB: Restraint stress in biomedical research: a review. Neurosci Biobehav Rev. 10:339–370. 1986.
14	Buynitsky T and Mostofsky D: Restraint stress in biobehavioral research: recent developments. Neurosci Biobehav Rev. 33:1089–1098. 2009. View Article : Google Scholar : PubMed/NCBI
15	Nakajima K, Hamada N, Takahashi Y, Sasaguri K, Tsukinoki K, Umemoto T and Sato S: Restraint stress enhances alveolar bone loss in an experimental rat model. J Periodontol Res. 41:527–534. 2006. View Article : Google Scholar : PubMed/NCBI
16	Semenoff Segundo A, Semenoff TA, Borges AH, Pedro FL and Sakai V: Methodological model of chronic stress associated with ligature-induced periodontitis in rats: a radiographic study. Braz Oral Res. 24:455–459. 2010.PubMed/NCBI
17	Saini R, Saini S and Saini SR: Periodontitis and psychological stress: a dental view. Ind Psychiatry J. 19:66–67. 2010. View Article : Google Scholar : PubMed/NCBI
18	Grissom N and Bhatnagar S: Habituation to repeated stress: get used to it. Neurobiol Learn Mem. 92:215–224. 2009. View Article : Google Scholar : PubMed/NCBI
19	Rivera CA, Droguett DA, Kemmerling U and Venegas BA: Chronic restraint stress in oral squamous cell carcinoma. J Dent Res. 90:799–803. 2011. View Article : Google Scholar : PubMed/NCBI
20	National Research Council, Institute for Laboratory Animal Resources: Guide for the Care and Use of Laboratory Animals. National Academy Press; Washington, DC: 1996
21	Olsson IA and Dahlborn K: Improving housing conditions for laboratory mice: a review of ‘environmental enrichment’. Lab Anim. 36:243–270. 2002.
22	Garcia MF: Estudio de la dinámica ósea mandibular y de los procesos reabsortivos de la cresta alveolar en ratas diabéticas y controles. Revista Académica Electrónica de la UNR 1: 1852-0707, 2008 (Available at: http://hdl.handle.net/2133/1509uri).
23	Liu L, Li C, Cai C, Xiang J and Cao Z: Cyclophilin A (CypA) is associated with the inflammatory infiltration and alveolar bone destruction in an experimental periodontitis. Biochem Biophys Res Commun. 391:1000–1006. 2010. View Article : Google Scholar : PubMed/NCBI
24	Liu R, Bal HS, Desta T, Krothapalli N, Alyassi M, Luan Q and Graves DT: Diabetes enhances periodontal bone loss through enhanced resorption and diminished bone formation. J Dent Res. 85:510–514. 2006. View Article : Google Scholar : PubMed/NCBI
25	Luan Q, Desta T, Chehab L, Sanders VJ, Plattner J and Graves DT: Inhibition of experimental periodontitis by a topical boron-based antimicrobial. J Dent Res. 87:148–152. 2008. View Article : Google Scholar : PubMed/NCBI
26	Graves DT, Naguib G, Lu H, Leone C, Hsue H and Krall E: Inflammation is more persistent in type 1 diabetic mice. J Dent Res. 84:324–328. 2005. View Article : Google Scholar : PubMed/NCBI
27	Lindhe J and Karring NT: Periodontología Clínica e Implantología Odontológica. 1. 5th edition. Editorial Medica Panamericana; Mexico: pp. 289–290. 2009