Comparison of the toxicity of aqueous and ethanol fractions of Angelica keiskei leaf using the eye irritancy test

  • Authors:
    • Hyeong-U Son
    • Eun-Kyung Yoon
    • Yong-Soo Cha
    • Min-A Kim
    • Yong-Kyu Shin
    • Jong-Myung Kim
    • Yong-Hee Choi
    • Sang-Han Lee
  • View Affiliations

  • Published online on: August 20, 2012     https://doi.org/10.3892/etm.2012.677
  • Pages: 820-824
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Abstract

To determine whether aqueous and ethanol fractions of the Angelica keiskei leaf exert toxicity when used for cosmetic purposes, we performed the acute eye irritancy test. Animals were treated with sample fractions (100 mg/dose) according to standard procedure guidelines. No significant changes or damage was detected in the fraction-treated groups in terms of ocular lesions in the cornea, the size of the cornea with turbidity, swelling of the eyelid and emission discharge. However, sodium dioctyl sulfosuccinate, a positive control, induced severe toxic symptoms. Thus, aqueous and ethanol fractions of Angelica keiskei do not appear to induce acute toxicity in the eye lens, as assessed from anatomical and pathological observations in the rabbit eye. Our results collectively suggest that aqueous and ethanol fractions show promise as cosmetic ingredients that do not cause eye toxicity.
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November 2012
Volume 4 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Son H, Yoon E, Cha Y, Kim M, Shin Y, Kim J, Choi Y and Lee S: Comparison of the toxicity of aqueous and ethanol fractions of Angelica keiskei leaf using the eye irritancy test. Exp Ther Med 4: 820-824, 2012
APA
Son, H., Yoon, E., Cha, Y., Kim, M., Shin, Y., Kim, J. ... Lee, S. (2012). Comparison of the toxicity of aqueous and ethanol fractions of Angelica keiskei leaf using the eye irritancy test. Experimental and Therapeutic Medicine, 4, 820-824. https://doi.org/10.3892/etm.2012.677
MLA
Son, H., Yoon, E., Cha, Y., Kim, M., Shin, Y., Kim, J., Choi, Y., Lee, S."Comparison of the toxicity of aqueous and ethanol fractions of Angelica keiskei leaf using the eye irritancy test". Experimental and Therapeutic Medicine 4.5 (2012): 820-824.
Chicago
Son, H., Yoon, E., Cha, Y., Kim, M., Shin, Y., Kim, J., Choi, Y., Lee, S."Comparison of the toxicity of aqueous and ethanol fractions of Angelica keiskei leaf using the eye irritancy test". Experimental and Therapeutic Medicine 4, no. 5 (2012): 820-824. https://doi.org/10.3892/etm.2012.677