Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November 2012 Volume 4 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November 2012 Volume 4 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients

  • Authors:
    • Masayuki Shitara
    • Hidefumi Sasaki
    • Keisuke Yokota
    • Katsuhiro Okuda
    • Yu Hikosaka
    • Satoru Moriyama
    • Motoki Yano
    • Tomoya Kawaguchi
    • Akihito Kubo
    • Minoru Takada
    • Naoto Kitahara
    • Meinoshin Okumura
    • Akihide Matsumura
    • Keiji Iuchi
    • Yoshitaka Fujii
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Immunology and Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan, Department of Internal Medicine, National Hospital Organization, Kinki-chuo Chest Medical Center, Sakai, Osaka 591-8555, Japan, Department of Surgery, National Hospital Organization, Kinki-chuo Chest Medical Center, Sakai, Osaka 591-8555, Japan
  • Pages: 785-789
    |
    Published online on: August 23, 2012
       https://doi.org/10.3892/etm.2012.681
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The epidermal growth factor receptor (EGFR) gene is highly polymorphic and its expression and activity may be affected by various polymorphisms. There have been several studies examining associations between EGFR polymorphisms and clinical outcome of lung cancer therapy; however, the underlying mechanism is largely unknown. The present study investigated EGFR polymorphism status and its correlation with clinicopathological features in Japanese non-small cell lung cancer (NSCLC) patients. We investigated 5 polymorphisms in the EGFR gene (-216G/T, -191C/A, 8227G/A, D994D and R497K) in 274 surgically-treated NSCLC patients. TaqMan single nucleotide polymorphism (SNP) genotyping assays and a PCR-based assay were used to analyze these polymorphisms. In our cohort of patients we did not find any evidence of the -191C/A polymorphism. Our results showed that the patients with the 8227GA or AA type in intron 1 had a significantly better prognosis with the anti-EGFR therapy than the patients with the GG type (p=0.0448) in terms of recurrence of lung cancer. No significant association was observed between 3 other SNPs (-216G/T, D994D and R497K) and clinicopathological features. The EGFR 8227G/A polymorphism in intron 1 may be associated with clinical outcome in NSCLC patients treated with EGFR tyrosine kinase inhibitors.
View Figures

Figure 1

Figure 2

View References

1 

Ginsberg RJ, Kris K and Armstrong G: Cancer of the lung. Principles and Practice of Oncology. DeVita VT, Hellman S and Rosenberg SA: 4th edition. JB Lippincott Co; Philadelphia, PA: pp. 673–682. 1993

2 

Nicholson RI, Gee JM and Harper ME: EGFR and cancer prognosis. Eur J Cancer. 37:S9–S15. 2001. View Article : Google Scholar

3 

Onn A, Choe DH, Herbst RS, et al: Synchronous overexpression of epidermal growth factor receptor and HER2-neu protein is a predictor of poor outcome in patients with stage I non-small cell lung cancer. Clin Cancer Res. 10:136–143. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Paez JG, Jänne PA, Lee JC, et al: EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy. Science. 304:1497–1500. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Lynch TJ, Bell DW, Sordella R, et al: Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. N Engl J Med. 350:2129–2139. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Fukuoka M, Wu Y-L, Thongprasert S, et al: Biomarker analyses and first overall survival results from a phase III, randomized, open-label, first-line study of gefitinib versus carboplatin/paclitaxel in clinically selected patients with advanced non-small-cell lung cancer in Asia (IPASS). J Clin Oncol. 29:2866–2874. 2011. View Article : Google Scholar

7 

Liu W, Innocenti F, Wu MH, et al: A functional common polymorphism in a Sp1 recognition site of the epidermal growth factor receptor gene promoter. Cancer Res. 65:46–53. 2005.PubMed/NCBI

8 

Liu W, Wu X, Zhang W, et al: Relationship of EGFR mutations, expression, amplification, and polymorphisms to epidermal growth factor receptor inhibitors in the NCI60 cell lines. Clin Cancer Res. 13:6788–6795. 2007. View Article : Google Scholar

9 

Brandt B, Meyer-Staeckling S, Schmidt H, et al: Mechanisms of EGFR gene transcription modulation: relationship to cancer risk and therapy response. Clin Cancer Res. 12:7252–7260. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Liu W, Innocenti F, Chen P, et al: Interethnic difference in the allelic distribution of human epidermal growth factor receptor intron 1 polymorphism. Clin Cancer Res. 9:1009–1012. 2003.PubMed/NCBI

11 

Ma F, Sun T, Shi Y, et al: Polymorphisms of EGFR predict clinical outcome in advanced non-small-cell lung cancer patients treated with Gefitinib. Lung Cancer. 66:114–119. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Liu G, Gurubhagavatula S, Zhou W, et al: Epidermal growth factor receptor polymorphisms and clinical outcomes in non-small-cell lung cancer patients treated with gefitinib. Pharmacogenomics J. 8:129–138. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Tiseo M, Capelletti M, De Palma G, et al: Epidermal growth factor receptor intron-1 polymorphism predicts gefitinib outcome in advanced non-small cell lung cancer. J Thorac Oncol. 3:1104–1111. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Jou YS, Lo YL, Hsiao CF, et al: Association of an EGFR intron 1 SNP with never smoking female lung adenocarcinoma patients. Lung Cancer. 64:251–256. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Endo K, Konishi A, Sasaki H, et al: Epidermal growth factor receptor gene mutation in non-small cell lung cancer using highly sensitive and fast TaqMan PCR assay. Lung Cancer. 50:375–384. 2005. View Article : Google Scholar : PubMed/NCBI

16 

De la Vega FM, Lazaruk KD, Rhodes MD and Wenz MH: Assessment of two flexible and compatible SNP genotyping platforms: TaqMan SNP Genotyping Assays and the SNPlex Genotyping System. Mutat Res. 573:111–135. 2005.PubMed/NCBI

17 

Sasaki H, Okuda K, Shimizu S, et al: EGFR R497K polymorphism is a favorable prognostic factor for advanced lung cancer. J Cancer Res Clin Oncol. 135:313–318. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Sasaki H, Shimizu S, Okuda K, et al: Epidermal growth factor receptor gene amplification in surgical resected Japanese lung cancer. Lung Cancer. 64:295–300. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Cappuzzo F, Hirsch FR, Rossi E, et al: Epidermal growth factor receptor gene and protein and gefitinib sensitivity in non-small-cell lung cancer. J Natl Cancer Inst. 97:643–655. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Sasaki H, Endo K, Takada M, et al: EGFR exon 20 insertion mutation in Japanese lung cancer. Lung Cancer. 58:324–328. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Nie Q, Wang Z, Zhang GC, et al: The epidermal growth factor receptor intron1 (CA) n microsatellite polymorphism is a potential predictor of treatment outcome in patients with advanced lung cancer treated with Gefitinib. Eur J Pharmacol. 570:175–181. 2007. View Article : Google Scholar

22 

Liu W, He L, Ramírez J, et al: Functional EGFR germline polymorphisms may confer risk for EGFR somatic mutations in non-small cell lung cancer, with a predominant effect on exon 19 microdeletions. Cancer Res. 71:2423–2427. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Suzuki M, Kageyama S, Shinmura K, et al: Inverse relationship between the length of the EGFR CA repeat polymorphism in lung carcinoma and protein expression of EGFR in the carcinoma. J Surg Oncol. 98:457–461. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Ichihara S, Toyooka S, Fujiwara Y, et al: The impact of epidermal growth factor receptor gene status on gefitinib-treated Japanese patients with non-small-cell lung cancer. Int J Cancer. 120:1239–1247. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Nomura M, Shigematsu H, Li L, et al: Polymorphisms, mutations, and amplification of the EGFR gene in non-small cell lung cancers. PLos One. 4:715–727. 2007.PubMed/NCBI

26 

Sasaki H, Okuda K, Takada M, et al: A novel EGFR mutation D1012H and polymorphism at exon 25 in Japanese lung cancer. J Cancer Res Clin Oncol. 134:1371–1376. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Girard N, Lou E, Azzoli CG, et al: Analysis of genetic variants in never-smokers with lung cancer facilitated by an internet-based blood collection protocol: a preliminary report. Clin Cancer Res. 16:755–763. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shitara M, Sasaki H, Yokota K, Okuda K, Hikosaka Y, Moriyama S, Yano M, Kawaguchi T, Kubo A, Takada M, Takada M, et al: Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients . Exp Ther Med 4: 785-789, 2012.
APA
Shitara, M., Sasaki, H., Yokota, K., Okuda, K., Hikosaka, Y., Moriyama, S. ... Fujii, Y. (2012). Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients . Experimental and Therapeutic Medicine, 4, 785-789. https://doi.org/10.3892/etm.2012.681
MLA
Shitara, M., Sasaki, H., Yokota, K., Okuda, K., Hikosaka, Y., Moriyama, S., Yano, M., Kawaguchi, T., Kubo, A., Takada, M., Kitahara, N., Okumura, M., Matsumura, A., Iuchi, K., Fujii, Y."Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients ". Experimental and Therapeutic Medicine 4.5 (2012): 785-789.
Chicago
Shitara, M., Sasaki, H., Yokota, K., Okuda, K., Hikosaka, Y., Moriyama, S., Yano, M., Kawaguchi, T., Kubo, A., Takada, M., Kitahara, N., Okumura, M., Matsumura, A., Iuchi, K., Fujii, Y."Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients ". Experimental and Therapeutic Medicine 4, no. 5 (2012): 785-789. https://doi.org/10.3892/etm.2012.681
Copy and paste a formatted citation
x
Spandidos Publications style
Shitara M, Sasaki H, Yokota K, Okuda K, Hikosaka Y, Moriyama S, Yano M, Kawaguchi T, Kubo A, Takada M, Takada M, et al: Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients . Exp Ther Med 4: 785-789, 2012.
APA
Shitara, M., Sasaki, H., Yokota, K., Okuda, K., Hikosaka, Y., Moriyama, S. ... Fujii, Y. (2012). Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients . Experimental and Therapeutic Medicine, 4, 785-789. https://doi.org/10.3892/etm.2012.681
MLA
Shitara, M., Sasaki, H., Yokota, K., Okuda, K., Hikosaka, Y., Moriyama, S., Yano, M., Kawaguchi, T., Kubo, A., Takada, M., Kitahara, N., Okumura, M., Matsumura, A., Iuchi, K., Fujii, Y."Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients ". Experimental and Therapeutic Medicine 4.5 (2012): 785-789.
Chicago
Shitara, M., Sasaki, H., Yokota, K., Okuda, K., Hikosaka, Y., Moriyama, S., Yano, M., Kawaguchi, T., Kubo, A., Takada, M., Kitahara, N., Okumura, M., Matsumura, A., Iuchi, K., Fujii, Y."Polymorphisms in intron 1 of the EGFR gene in non‑small cell lung cancer patients ". Experimental and Therapeutic Medicine 4, no. 5 (2012): 785-789. https://doi.org/10.3892/etm.2012.681
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team