Role of NEDD9 in invasion and metastasis of lung adenocarcinoma

  • Authors:
    • Jing-Xia Chang
    • Feng Gao
    • Guo-Qiang Zhao
    • Guo-Jun Zhang
  • View Affiliations

  • Published online on: September 3, 2012     https://doi.org/10.3892/etm.2012.693
  • Pages: 795-800
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Abstract

Treatment failure for lung adenocarcinoma is frequently due to lymph node metastasis and invasion to neighboring organs. The aim of the present study was to investigate the invasion- and metastasis-related gene, neural precursor cell expressed, developmentally downregulated 9 (NEDD9), in lung adenocarcinoma tissues and cell lines. The expression of NEDD9 was analyzed by the SP method of immunohistochemistry for 60 formalin-fixed and paraffin-embedded (FFPE) lung adenocarcinoma tissues in which 32 cases were metastastic and 28 were without metastases. NEDD9 mRNA expression and protein levels were quantified by fluorescence quantitative reverse transcription-polymerase chain reaction (FQ-PCR) and western blotting in the highly invasive lung adenocarcinoma cell lines A549 and 95D as well as in SPC-A-1 cells with low invasive potential. The immunostaining scores revealed a statistically significant difference between metastatic and non-metastatic lung adenocarcinomas (p<0.001). FQ-PCR and western blotting demonstrated that NEDD9 expression was higher in A549 and 95D compared to SPC-A-1 cells (P=0.003). Our results provide evidence that NEDD9 is upregulated in metastatic lung adenocarcinoma and in highly invasive lung adenocarcinoma cell lines, suggesting its potential involvement in regulating cell migration and invasion.
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November 2012
Volume 4 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chang J, Gao F, Zhao G and Zhang G: Role of NEDD9 in invasion and metastasis of lung adenocarcinoma . Exp Ther Med 4: 795-800, 2012
APA
Chang, J., Gao, F., Zhao, G., & Zhang, G. (2012). Role of NEDD9 in invasion and metastasis of lung adenocarcinoma . Experimental and Therapeutic Medicine, 4, 795-800. https://doi.org/10.3892/etm.2012.693
MLA
Chang, J., Gao, F., Zhao, G., Zhang, G."Role of NEDD9 in invasion and metastasis of lung adenocarcinoma ". Experimental and Therapeutic Medicine 4.5 (2012): 795-800.
Chicago
Chang, J., Gao, F., Zhao, G., Zhang, G."Role of NEDD9 in invasion and metastasis of lung adenocarcinoma ". Experimental and Therapeutic Medicine 4, no. 5 (2012): 795-800. https://doi.org/10.3892/etm.2012.693