Molecular typing of Mycobacterium tuberculosis isolates circulating in Henan, central China

Zhao,Yuling; Li,Hui; Xing,Jin; Yang,Hongyi; Ma,Xiaoguang; Xu,Jiying; Shi,Jie; Yan,Guorui

doi:10.3892/etm.2012.699

Molecular typing of Mycobacterium tuberculosis isolates circulating in Henan, central China

Authors:
- Yuling Zhao
- Hui Li
- Jin Xing
- Hongyi Yang
- Xiaoguang Ma
- Jiying Xu
- Jie Shi
- Guorui Yan
View Affiliations

Affiliations: Center for Disease Control and Prevention of Henan Province, Zhengzhou, Henan 450016, P.R. China
Published online on: September 6, 2012 https://doi.org/10.3892/etm.2012.699
Pages: 949-953

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to characterize the genotypes of Mycobacterium tuberculosis (MT) in isolates obtained from Henan, China, and to study the distribution features of Beijing strains in Henan. A total of 443 MT strains isolated in Henan Province were tested for susceptibility to isonicotinylhydrazide (INH), rifampicin (RFP), ethambutol (EMB) and streptomycin (SM), and genotyped by spoligotyping. The clustering of genotypes revealed 4 gene clusters (Beijing and Beijing-like, T, Manu, and S and LAM3) and 24 genotypes. In total, 387 (87.4%) of the strains were Beijing strains. The frequency of multidrug-resistant (MDR) strains was significantly higher in the Beijing and Beijing-like strains than in the other strains (χ2=4.6564, P<0.05). However, the percentages of drug resistance and sensitivity in the Beijing strains were almost the same as those in the non-Beijing strains. The proportion of Beijing strains in the ≤60-year-old TB patients was significantly higher than in the >60‑year‑old TB patients (χ2=32.053, P<0.001). The distribution of Beijing strains deceased gradually from the east to the west in Henan Province (P<0.0001). The data demonstrate that the Beijing genotype is a major type in the area and may be related to enhanced transmissibility. The transmission of Beijing family strains has increased in Henan and its incidence is highest in the east of Henan. The MDR strains were significant in the spread of MT.

View Figures

View References

1	Thomas SK, Iravatham CC, Moni BH, et al: Modern and ancestral genotypes of Mycobacterium tuberculosis from Andhra Pradesh, India. PLoS One. 6:e275842011.PubMed/NCBI
2	Kamerbeek J, Schouls L, Kolk A, et al: Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 35:907–914. 1997.PubMed/NCBI
3	van Soolingen D, Borgdorff MW, de Haas PE, et al: Molecular epidemiology of tuberculosis in the Netherlands: a nationwide study from 1993 through 1997. J Infect Dis. 180:726–736. 1999.PubMed/NCBI
4	Driscoll JR: Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex. Methods Mol Biol. 551:117–128. 2009. View Article : Google Scholar : PubMed/NCBI
5	Sola C, Filliol I, Legrand E, Mokrousov I and Rastogi N: Mycobacterium tuberculosis phylogeny reconstruction based on combined numerical analysis with IS1081, IS6110, VNTR, and DR-based spoligotyping suggests the existence of two new phylogeographical clades. J Mol Evol. 53:680–689. 2001. View Article : Google Scholar
6	Brudey K, Driscoll JR, Rigouts L, et al: Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol. 6:232006. View Article : Google Scholar
7	van Soolingen D, Qian L, de Haas PE, et al: Predominance of a single genotype of Mycobacterium tuberculosis in countries of east Asia. J Clin Microbiol. 33:3234–3238. 1995.PubMed/NCBI
8	Roetzer A, Schuback S, Diel R, et al: Evaluation of Mycobacterium tuberculosis typing methods in a 4-year study in Schleswig-Holstein, Northern Germany. J Clin Microbiol. 49:4173–4178. 2011.PubMed/NCBI
9	Brosch R, Gordon SV, Marmiesse M, et al: A new evolutionary scenario for the Mycobacterium tuberculosis complex. Proc Natl Acad Sci USA. 99:3684–3689. 2002. View Article : Google Scholar : PubMed/NCBI
10	Abebe F and Bjune G: The emergence of Beijing family genotypes of Mycobacterium tuberculosis and low-level protection by bacille Calmette-Guérin (BCG) vaccines: is there a link? Clin Exp Immunol. 145:389–397. 2006.PubMed/NCBI
11	Bifani PJ, Mathema B, Kurepina NE and Kreiswirth BN: Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains. Trends Microbiol. 10:45–52. 2002.
12	Glynn JR, Whiteley J, Bifani PJ, Kremer K and van Soolingen D: Worldwide occurrence of Beijing/W strains of Mycobacterium tuberculosis: a systematic review. Emerg Infect Dis. 8:843–849. 2002. View Article : Google Scholar : PubMed/NCBI
13	Diaz R, Kremer K, de Haas PE, et al: Molecular epidemiology of tuberculosis in Cuba outside of Havana, July 1994–June 1995: utility of spoligotyping versus IS6110 restriction fragment length polymorphism. Int J Tuberc Lung Dis. 2:743–750. 1998.PubMed/NCBI
14	Cole ST, Brosch R, Parkhill J, et al: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 393:537–544. 1998.
15	Filliol I, Driscoll JR, van Soolingen D, et al: Global distribution of Mycobacterium tuberculosis spoligotypes. Emerg Infect Dis. 8:1347–1349. 2002.
16	López B, Aguilar D, Orozco H, et al: A marked difference in pathogenesis and immune response induced by different Mycobacterium tuberculosis genotypes. Clin Exp Immunol. 133:30–37. 2003.PubMed/NCBI
17	European Concerted Action on New Generation Genetic Markers and Techniques for the Epidimiology and Control of Tuberculosis: Beijing/W genotype Mycobacterium tuberculosis and drug resistance. Emerg Infect Dis. 12:736–743. 2006.
18	Parwati I, van Crevel R and van Soolingen D: Possible underlying mechanisms for successful emergence of the Mycobacterium tuberculosis Beijing genotype strains. Lancet Infect Dis. 10:103–111. 2010. View Article : Google Scholar : PubMed/NCBI
19	World Health Organization (WHO)/International Union Against Tuberculosis and Lung Disease (IUATLD): Global working group on antituberculosis drug resistance surveillance [S]. Guidelines for surveillance of drug resistance in tuberculosis. 1997
20	Anh DD, Borgdorff MW, Van LN, et al: Mycobacterium tuberculosis Beijing genotype emerging in Vietnam. Emerg Infect Dis. 6:302–305. 2000. View Article : Google Scholar
21	Dou HY, Tseng FC, Lin CW, et al: Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei. BMC Infect Dis. 8:1702008. View Article : Google Scholar : PubMed/NCBI
22	Liu Q, Yang D, Xu W, et al: Molecular typing of Mycobacterium tuberculosis isolates circulating in Jiangsu province, China. BMC Infect Dis. 11:2882011.