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Article

Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase

  • Authors:
    • Yi-E Huang
    • Zhi-Han Tang
    • Wei Xie
    • Xin-Tian Shen
    • Mi-Hua Liu
    • Xiang-Ping Peng
    • Zhan-Zhi Zhao
    • De-Bo Nie
    • Lu-Shan Liu
    • Zhi-Sheng Jiang
  • View Affiliations / Copyright

    Affiliations: Institute of Cardiovascular Disease and Key Laboratory for Arteriosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, P.R. China, Huaihua Medical College, Huaihua, Hunan 418000, P.R. China
  • Pages: 1117-1123
    |
    Published online on: October 1, 2012
       https://doi.org/10.3892/etm.2012.733
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Abstract

Hydrogen sulfide (H2S), produced by cystanthionine-γ-lysase (CSE) in the cardiovascular system, has been suggested to be the third gasotransmitter in addition to nitric oxide (NO) and carbon monoxide (CO). The present study aimed to investigate the role of H2S in ischemic postconditioning (IPO) during the early period of reperfusion. IPO with 6 episodes of 10 sec reperfusion followed by 6 episodes of 10 sec ischemia (IPO 2') was administered when reperfusion was initiated. Cardiodynamics and the concentration of H2S were measured at 1, 2, 3, 4, 5, 10, 20, 30, 60, 90 and 120 min of reperfusion. Lactate dehydrogenase (LDH) levels and infarct size were determined at the end of the reperfusion. The concentration of H2S was stable during the whole experiment in the control group, whereas it reached a peak at the first minute of reperfusion in the ischemia-reperfusion (IR) group. The concentration of H2S at the first minute of reperfusion in the IPO 2' group was higher compared to that of the IR group, which correlated with cardioprotection including improved heart contractile function and reduced infarct size and LDH levels. However, the above effects of IPO 2' were attenuated by pre-treatment with blockade of endogenous H2S production with DL-propargylglycine for 20 min prior to global ischemia. Furthermore, we found that other forms of IPO, IPO commencing at 1 min after reperfusion (delayed IPO) or lasting only for 1 min (IPO 1'), failed to increase the concentration of H2S and protect the myocardium. We conclude that the peak of endogenous H2S in the early reperfusion phase is the key to cardioprotection induced by IPO.
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Copy and paste a formatted citation
Spandidos Publications style
Huang Y, Tang Z, Xie W, Shen X, Liu M, Peng X, Zhao Z, Nie D, Liu L, Jiang Z, Jiang Z, et al: Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase. Exp Ther Med 4: 1117-1123, 2012.
APA
Huang, Y., Tang, Z., Xie, W., Shen, X., Liu, M., Peng, X. ... Jiang, Z. (2012). Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase. Experimental and Therapeutic Medicine, 4, 1117-1123. https://doi.org/10.3892/etm.2012.733
MLA
Huang, Y., Tang, Z., Xie, W., Shen, X., Liu, M., Peng, X., Zhao, Z., Nie, D., Liu, L., Jiang, Z."Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase". Experimental and Therapeutic Medicine 4.6 (2012): 1117-1123.
Chicago
Huang, Y., Tang, Z., Xie, W., Shen, X., Liu, M., Peng, X., Zhao, Z., Nie, D., Liu, L., Jiang, Z."Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase". Experimental and Therapeutic Medicine 4, no. 6 (2012): 1117-1123. https://doi.org/10.3892/etm.2012.733
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Tang Z, Xie W, Shen X, Liu M, Peng X, Zhao Z, Nie D, Liu L, Jiang Z, Jiang Z, et al: Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase. Exp Ther Med 4: 1117-1123, 2012.
APA
Huang, Y., Tang, Z., Xie, W., Shen, X., Liu, M., Peng, X. ... Jiang, Z. (2012). Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase. Experimental and Therapeutic Medicine, 4, 1117-1123. https://doi.org/10.3892/etm.2012.733
MLA
Huang, Y., Tang, Z., Xie, W., Shen, X., Liu, M., Peng, X., Zhao, Z., Nie, D., Liu, L., Jiang, Z."Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase". Experimental and Therapeutic Medicine 4.6 (2012): 1117-1123.
Chicago
Huang, Y., Tang, Z., Xie, W., Shen, X., Liu, M., Peng, X., Zhao, Z., Nie, D., Liu, L., Jiang, Z."Endogenous hydrogen sulfide mediates the cardioprotection induced by ischemic postconditioning in the early reperfusion phase". Experimental and Therapeutic Medicine 4, no. 6 (2012): 1117-1123. https://doi.org/10.3892/etm.2012.733
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