Changes in the expression of hippocampal proteins in rats with recrudescence of morphine addiction

Qiu,Haitang; Gao,Yufeng; Fu,Yixiao; Du,Lian; Qiu,Tian; Feng,Kun; Luo,Qinghua; Meng,Huaqing

doi:10.3892/etm.2012.861

March 2013 Volume 5 Issue 3

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March 2013 Volume 5 Issue 3

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Article

Changes in the expression of hippocampal proteins in rats with recrudescence of morphine addiction

Authors:
- Haitang Qiu
- Yufeng Gao
- Yixiao Fu
- Lian Du
- Tian Qiu
- Kun Feng
- Qinghua Luo
- Huaqing Meng
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Affiliations: Department of Mental Health, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Second Department, Third Hospital of Zhongshan, Zhongshan 510630, P.R. China, Department of Mental Health, Yuquan Hospital of Tsinghua University, Beijing 100049, P.R. China
Pages: 825-829
|
Published online on: December 18, 2012

https://doi.org/10.3892/etm.2012.861
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Abstract

The high recrudescence rate of drug addiction has received attention worldwide and its mechanisms remain to be elucidated. This study aimed to analyse the disparate protein expression in the hippocampal tissue of rats with recrudescence of morphine addiction, as well as to provide clues for the exploration of the recrudescence mechanism. Sixteen male adult Sprague-Dawley rats were divided equally into the morphine and physiological saline groups. Effective nose pokes were determined as the main index. The proteins were separated using the immobilised pH gradient two-dimensional polyacrylamide gel electrophoresis (2-DE). Disparate protein spots were analysed using the PDQuest 2-DE software. Peptide dactylograms were obtained using the matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The effective nose poke counts of the morphine group significantly increased during addiction maturation compared with the saline group (P<0.001). The post-recrudescence nose poke counts of the morphine group significantly increased compared with those before recrudescence (P<0.001). Fifteen disparate proteins were identified according to the protein electrophoresis of the morphine and physiological saline groups, including three proteins associated with energy metabolism, two ionic channel regulatory proteins, one heat shock protein and one exogenous substance metabolic enzyme. The energy metabolism and expression of cell metabolism-related proteins decreased in the hippocampus of rats with morphine recrudescence.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Changes in the expression of hippocampal proteins in rats with recrudescence of morphine addiction

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