Effect of advanced glycation end products on the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor proteins in RF/6A cells

  • Authors:
    • Huiming Zhang
    • Luosheng Tang
    • Siying Chen
    • Yezhen Yang
    • Mingjiazi Chen
    • Jing Luo
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  • Published online on: March 15, 2013     https://doi.org/10.3892/etm.2013.1015
  • Pages: 1519-1522
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Abstract

The aim of this study was to investigate the effect of advanced glycation end products (AGEs) on the expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) proteins in RF/6A cells cultured in vitro, and to investigate the association between the expression of HIF-1α and VEGF proteins. RF/6A cells were cultured in vitro and treated with AGEs and non-glycated albumin control at various concentrations (0, 50, 100, 200, 400 and 800 mg/l) for 24 h. The expression of the VEGF protein was detected by ELISA, and western blot analysis was used to determine the levels of HIF-1α protein. The expression of HIF-1α and VEGF proteins was significantly higher in the AGE group compared with the non-glycated control group (all P<0.05). With the increase in concentration of AGEs, the expression levels of HIF-1α and VEGF protein increased and reached a maximum at 200 mg/l AGE, then decreased at 400 and 800 mg/l. However this effect was not observed in the non‑glycated control groups. There was a positive correlation between the expression of HIF-1α and VEGF (P<0.05). AGEs induced the expression of HIF-1α and VEGF proteins in RF/6A cells in a concentration‑dependent manner. AGEs may upregulate the expression of VEGF protein by increasing the levels of HIF-1α protein, demonstrating the potential role of HIF-1α-targeted therapy in neovascularization.
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May 2013
Volume 5 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang H, Tang L, Chen S, Yang Y, Chen M and Luo J: Effect of advanced glycation end products on the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor proteins in RF/6A cells. Exp Ther Med 5: 1519-1522, 2013
APA
Zhang, H., Tang, L., Chen, S., Yang, Y., Chen, M., & Luo, J. (2013). Effect of advanced glycation end products on the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor proteins in RF/6A cells. Experimental and Therapeutic Medicine, 5, 1519-1522. https://doi.org/10.3892/etm.2013.1015
MLA
Zhang, H., Tang, L., Chen, S., Yang, Y., Chen, M., Luo, J."Effect of advanced glycation end products on the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor proteins in RF/6A cells". Experimental and Therapeutic Medicine 5.5 (2013): 1519-1522.
Chicago
Zhang, H., Tang, L., Chen, S., Yang, Y., Chen, M., Luo, J."Effect of advanced glycation end products on the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor proteins in RF/6A cells". Experimental and Therapeutic Medicine 5, no. 5 (2013): 1519-1522. https://doi.org/10.3892/etm.2013.1015