Histological and mechanical properties of autologous living tissue biotubes

Chen,Xiao‑Song; Ou,Tong‑Wen; Zhang,Jian; Li,Jian‑Xin; Chen,Bing; Yu,Heng‑Xi; Gu,Yong‑Quan; Cui,Ye‑Qing; Zhang,Jing‑Yan; Xu,Yan‑Ling; Sun,Han‑Chen; Liu,Shuang; Wang,Rong

doi:10.3892/etm.2013.1040

Histological and mechanical properties of autologous living tissue biotubes

Authors:
- Xiao‑Song Chen
- Tong‑Wen Ou
- Jian Zhang
- Jian‑Xin Li
- Bing Chen
- Heng‑Xi Yu
- Yong‑Quan Gu
- Ye‑Qing Cui
- Jing‑Yan Zhang
- Yan‑Ling Xu
- Han‑Chen Sun
- Shuang Liu
- Rong Wang
View Affiliations

Affiliations: Department of Urology, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China, Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China, Central Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China
Published online on: April 2, 2013 https://doi.org/10.3892/etm.2013.1040
Pages: 1613-1618

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to explore and evaluate biotubes consisting of autologous tissues. The biotubes were prepared by intra-abdominally embedding silicon rods as moulds. The specimens were analyzed by mechanical tests, histological observation and superficial study. The intra-abdominal implantation of the silicone tubes readily stimulated the development of the biotubes. The biotubes consisted of collagen‑rich extracellular matrices. Myofibroblasts appeared as elongated cells with circumferential or longitudinal orientations. Subsequent to one month of embedding, the thickness of the tube wall was 70‑250 µm. The burst strength was 1100±187 mmHg and the suturability was excellent. Biotubes that have the ability to be widely variable in their shapes are composed of autologous cells and glomerular extracellular matrices. Biotubes are ideal grafts for tissue engineering as they are able to avoid immunological rejection and are of sufficient mechanical strength.

View Figures

View References

1.	Peirce EC II: Autologous tissue tubes for aortic grafts in dogs. Surgery. 33:648–657. 1953.PubMed/NCBI
2.	Sparks CH, Melgard MA and Raaf J: Carotid artery replacement with reinforced autogenous vein grafts. Angiology. 14:542–551. 1963. View Article : Google Scholar : PubMed/NCBI
3.	Parsonnet V, Alpert J and Brief DK: Autogenous polypropylene-supported collagen tubes for long-term arterial replacement. Surgery. 70:935–939. 1971.PubMed/NCBI
4.	Sparks CH: Silicone mandril method of femoropopliteal artery bypass. Clinical experience and surgical technics. Am J Surg. 124:244–249. 1972. View Article : Google Scholar : PubMed/NCBI
5.	Sparks CH: Silicone mandril method for growing reinforced autogenous femoro-popliteal artery grafts in situ. Ann Surg. 177:293–300. 1973. View Article : Google Scholar : PubMed/NCBI
6.	McKee JA, Banik SS, Boyer MJ, et al: Human arteries engineered in vitro. EMBO Rep. 4:633–638. 2003. View Article : Google Scholar : PubMed/NCBI
7.	Isenberg BC, Williams C and Tranquillo RT: Small-diameter artificial arteries engineered in vitro. Circ Res. 98:25–35. 2006. View Article : Google Scholar : PubMed/NCBI
8.	Campbell GR and Campbell JH: Development of tissue engineered vascular grafts. Curr Pharm Biotechnol. 8:43–50. 2007. View Article : Google Scholar : PubMed/NCBI
9.	Bailey MT, Pillarisetti S, Xiao H and Vyavahare NR: Role of elastin in pathologic calcification of xenograft heart valves. J Biomed Mater Res A. 66:93–102. 2003. View Article : Google Scholar : PubMed/NCBI
10.	Kakisis JD, Liapis CD, Breuer C and Sumpio BE: Artificial blood vessel: the Holy Grail of peripheral vascular surgery. J Vasc Surg. 41:349–354. 2005. View Article : Google Scholar : PubMed/NCBI
11.	L’Heureux N, Dusserre N, Konig G, et al: Human tissue-engineered blood vessel for adult arterial revascularization. Nat Med. 12:361–365. 2006.PubMed/NCBI
12.	Konig G, McAllister TN, Dusserre N, et al: Mechanical properties of completely autologous human tissue engineered blood vessels compared to human saphenous vein and mammary artery. Biomaterials. 30:1542–1550. 2009. View Article : Google Scholar
13.	Higgins SP, Solan AK and Niklason LE: Effects of polyglycolic acid on porcine smooth muscle cell growth and differentiation. J Biomed Mater Res A. 67:295–302. 2003. View Article : Google Scholar : PubMed/NCBI
14.	Shekhonin BV, Domogatsky SP, Muzykantov VR, Idelson GL and Rukosuev VS: Distribution of type I, III, IV and V collagen in normal and atherosclerotic human arterial wall: immunomorphological characteristics. Coll Relat Res. 5:355–368. 1985. View Article : Google Scholar
15.	Voss B and Rauterberg J: Localization of collagen types I, III, IV and V, fibronectin and laminin in human arteries by the indirect immunofluorescence method. Pathol Res Pract. 181:568–575. 1986. View Article : Google Scholar : PubMed/NCBI
16.	Morton LF and Barnes MJ: Collagen polymorphism in the normal and diseased blood vessel wall. Investigation of collagens types I, III and V. Atherosclerosis. 42:41–51. 1982. View Article : Google Scholar : PubMed/NCBI
17.	Canham PB, Finlay HM, Kiernan JA and Ferguson GG: Layered structure of saccular aneurysms assessed by collagen birefringence. Neurol Res. 21:618–626. 1999.PubMed/NCBI
18.	Hou Y, Mao Z, Wei X, et al: The roles of TGF-beta1 gene transfer on collagen formation during Achilles tendon healing. Biochem Biophys Res Commun. 383:235–239. 2009. View Article : Google Scholar : PubMed/NCBI
19.	Kaemmer D, Bozkurt A, Otto J, et al: Evaluation of tissue components in the peripheral nervous system using Sirius red staining and immunohistochemistry: a comparative study (human, pig, rat). J Neurosci Methods. 190:112–116. 2010. View Article : Google Scholar : PubMed/NCBI
20.	Chen C, Loe F, Blocki A, Peng Y and Raghunath M: Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies. Adv Drug Deliv Rev. 63:277–290. 2011. View Article : Google Scholar : PubMed/NCBI
21.	Kim BS, Park IK, Hoshiba T, Jiang HL, Choi YJ, Akaike T and Cho CS: Design of artificial extracellular matrices for tissue engineering. Prog Polym Sci. 36:238–268. 2011. View Article : Google Scholar
22.	Elliott JT, Woodward JT, Langenbach KJ, Tona A, Jones PL and Plant AL: Vascular smooth muscle cell response on thin films of collagen. Matrix Biol. 24:489–502. 2005. View Article : Google Scholar : PubMed/NCBI
23.	Critser PJ, Kreger ST, Voytik-Harbin SL and Yoder MC: Collagen matrix physical properties modulate endothelial colony forming cell-derived vessels in vivo. Microvasc Res. 80:23–30. 2010. View Article : Google Scholar : PubMed/NCBI