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Article

Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation

  • Authors:
    • Xiao Ke
    • Jingtuan Chen
    • Xin Zhang
    • Wenyi Fang
    • Chunbo Yang
    • Jun Peng
    • Youqin Chen
    • Thomas J. Sferra
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350003, P.R. China, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350122, P.R. China, Rainbow Babies and Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
  • Pages: 189-193
    |
    Published online on: April 22, 2013
       https://doi.org/10.3892/etm.2013.1071
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Abstract

Ulcerative colitis (UC) is a major form of inflammatory bowel disease (IBD), which is tightly regulated by the nuclear factor κB (NF-κB) pathway. Thus, the suppression of NF-κB signaling may provide a promising strategy for the treatment of UC. Qing Hua Chang Yin (QHCY) is a traditional Chinese formulation, which has been used for a number of years to clinically treat UC. However, little is known with regard to its anti-inflammatory properties. In the present study, lipopolysaccharide (LPS)-stimulated Caco-2 cells were used as an in vitro inflammatory model of the human intestinal epithelium to evaluate the anti-inflammatory effects of QHCY and its underlying molecular mechanisms. We observed that QHCY inhibited the inflammatory response in intestinal epithelial cells as it significantly and concentration-dependently reduced the LPS-induced secretion of pro-inflammatory TNF-α and IL-8 in Caco-2 cells. Furthermore, QHCY treatment inhibited the phosphorylation of IκB and the nuclear translocation of NF-κB in Caco-2 cells in a concentration‑dependent manner, indicating that QHCY suppressed the activation of the NF-κB signaling pathway. Collectively, our results suggest that the inhibition of NF-κB‑mediated inflammation may constitute a potential mechanism by which QHCY treats UC.
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Copy and paste a formatted citation
Spandidos Publications style
Ke X, Chen J, Zhang X, Fang W, Yang C, Peng J, Chen Y and Sferra TJ: Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation. Exp Ther Med 6: 189-193, 2013.
APA
Ke, X., Chen, J., Zhang, X., Fang, W., Yang, C., Peng, J. ... Sferra, T.J. (2013). Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation. Experimental and Therapeutic Medicine, 6, 189-193. https://doi.org/10.3892/etm.2013.1071
MLA
Ke, X., Chen, J., Zhang, X., Fang, W., Yang, C., Peng, J., Chen, Y., Sferra, T. J."Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation". Experimental and Therapeutic Medicine 6.1 (2013): 189-193.
Chicago
Ke, X., Chen, J., Zhang, X., Fang, W., Yang, C., Peng, J., Chen, Y., Sferra, T. J."Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation". Experimental and Therapeutic Medicine 6, no. 1 (2013): 189-193. https://doi.org/10.3892/etm.2013.1071
Copy and paste a formatted citation
x
Spandidos Publications style
Ke X, Chen J, Zhang X, Fang W, Yang C, Peng J, Chen Y and Sferra TJ: Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation. Exp Ther Med 6: 189-193, 2013.
APA
Ke, X., Chen, J., Zhang, X., Fang, W., Yang, C., Peng, J. ... Sferra, T.J. (2013). Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation. Experimental and Therapeutic Medicine, 6, 189-193. https://doi.org/10.3892/etm.2013.1071
MLA
Ke, X., Chen, J., Zhang, X., Fang, W., Yang, C., Peng, J., Chen, Y., Sferra, T. J."Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation". Experimental and Therapeutic Medicine 6.1 (2013): 189-193.
Chicago
Ke, X., Chen, J., Zhang, X., Fang, W., Yang, C., Peng, J., Chen, Y., Sferra, T. J."Qing Hua Chang Yin attenuates lipopolysaccharide-induced inflammatory response in human intestinal cells by inhibiting NF-κB activation". Experimental and Therapeutic Medicine 6, no. 1 (2013): 189-193. https://doi.org/10.3892/etm.2013.1071
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