Effect of folic acid and vitamin B12 on the expression of PPARγ, caspase‑3 and caspase‑8 mRNA in the abdominal aortas of rats with hyperlipidemia

Lv,Feng‑Hua; Gao,Jian‑Zhi; Teng,Qing‑Lei; Zhang,Jin‑Ying

doi:10.3892/etm.2013.1076

July 2013 Volume 6 Issue 1

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July 2013 Volume 6 Issue 1

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Article

Effect of folic acid and vitamin B12 on the expression of PPARγ, caspase‑3 and caspase‑8 mRNA in the abdominal aortas of rats with hyperlipidemia

Authors:
- Feng‑Hua Lv
- Jian‑Zhi Gao
- Qing‑Lei Teng
- Jin‑Ying Zhang
View Affiliations / Copyright

Affiliations: Department of Cardiology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Cardiology, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China, Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China
Pages: 184-188
|
Published online on: April 24, 2013

https://doi.org/10.3892/etm.2013.1076
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Abstract

Hyperlipidemia may lead to endothelial injury, due to its effects on homocysteine and vascular endothelial growth factor in the serum, and the mRNA expression levels of peroxisome proliferator‑activated receptor‑γ (PPARγ), and caspase‑3 and ‑8 in the vascular wall. In order to prevent and mitigate the high‑fat state that results from endothelial injury, this study examined the effect of folic acid (FA) and vitamin B12 (VB12) on the expression of PPARγ and caspase‑3 and ‑8 mRNA in the abdominal aortas of rats with hyperlipidemia. Sixty 4‑week‑old healthy male Sprague Dawley rats were randomly divided into five groups (each n=12): the normal control (NC), high‑fat diet (HL), FA, VB12 and FA+VB12 groups. Following one week of adaptive feeding, the FA, VB12 and FA+VB12 groups were subject to the intraperitoneal injection of FA (0.5 mg/day), VB12 (0.05 mg/day) and FA+VB12 (0.5 mg/day and 0.05 mg/day), respectively, while fed a high‑fat diet. The rats in the NC group were injected intraperitoneally with 0.9% NaCl solution (0.5 ml/day) and fed a normal diet, whereas those in the HL group were fed a high‑fat diet only. A reverse transcription‑polymerase chain reaction (RT‑PCR) assay demonstrated that at the end of week 12, the FA treatment had effectively increased the PPARγ mRNA level, while reducing the caspase‑3 and ‑8 mRNA levels, compared with the high‑fat diet treatment (P<0.05). The effect of FA on the expression of PPARγ and caspase‑3 and ‑8 was enhanced when used in combination with VB12 (P<0.05). These results revealed that the application of FA, alone or in combination with VB12, improves and mitigates the high‑fat state that results from endothelial injury.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of folic acid and vitamin B12 on the expression of PPARγ, caspase‑3 and caspase‑8 mRNA in the abdominal aortas of rats with hyperlipidemia

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