Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration

  • Authors:
    • Kai Jin
    • Dong-Dong Ren
    • Fang-Lu Chi
    • Juan-Mei Yang
    • Yi-Bo Huang
    • Wen Li
  • View Affiliations

  • Published online on: April 29, 2013     https://doi.org/10.3892/etm.2013.1089
  • Pages: 177-183
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Abstract

The aim of this study was to investigate the effects of actin depolymerizing factor (ADF)/destrin and position changes of kinetosomes in the development of hair cells following Atoh1-induced ectopic regeneration in the basilar membrane of mice. We observed through immunofluorescence at various time-points the expression of ADF/destrin and the specific kinetosome marker, γ-tubulin, in hair cells following ectopic regeneration induced by adenovirus transfection, overexpression of Atoh1 and in vitro culture. Changes of ADF/destrin distribution and kinetosome position during in vitro culture of new hair cells [Myo7a(+)] following Atoh1-induced ectopic regeneration are consistent with the changes in ADF/destrin expression and the polar migration of kinetosomes in hair cells of the cochlear sensory epithelium in normal development. ADF/destrin is involved in the development of the auditory epithelium and the development and structural rearrangement of ectopically regenerated hair cells in mammals. The kinetosomes of hair cells following Atoh1‑induced ectopic regeneration show positional changes in vitro at different time-points.
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July 2013
Volume 6 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Jin K, Ren D, Chi F, Yang J, Huang Y and Li W: Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration. Exp Ther Med 6: 177-183, 2013
APA
Jin, K., Ren, D., Chi, F., Yang, J., Huang, Y., & Li, W. (2013). Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration. Experimental and Therapeutic Medicine, 6, 177-183. https://doi.org/10.3892/etm.2013.1089
MLA
Jin, K., Ren, D., Chi, F., Yang, J., Huang, Y., Li, W."Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration". Experimental and Therapeutic Medicine 6.1 (2013): 177-183.
Chicago
Jin, K., Ren, D., Chi, F., Yang, J., Huang, Y., Li, W."Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration". Experimental and Therapeutic Medicine 6, no. 1 (2013): 177-183. https://doi.org/10.3892/etm.2013.1089