Primary testicular diffuse large B-cell lymphoma: A case report focusing on touch imprint cytology and a non-germinal center B-cell-like phenotype

Kim,Hyun-Soo

doi:10.3892/etm.2013.1091

July 2013 Volume 6 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

July 2013 Volume 6 Issue 1

Full Size Image

Case Report

Primary testicular diffuse large B-cell lymphoma: A case report focusing on touch imprint cytology and a non-germinal center B-cell-like phenotype

Authors:
- Hyun-Soo Kim
View Affiliations / Copyright

Affiliations: Molecular Biology Laboratory, Aerospace Medical Center, Republic of Korea Air Force, Cheongwon-gun, Chungcheongbuk-do 363-849, Republic of Korea
Pages: 33-36
|
Published online on: April 29, 2013

https://doi.org/10.3892/etm.2013.1091
Expand metrics +

Abstract

Primary diffuse large B-cell lymphoma (DLBCL) of the testis is a rare subtype of testicular tumor. While the histomorphology of testicular DLBCL is well described, a paucity of information in the literature exists with regard to the cytological diagnosis of this subtype of tumor. Touch imprint specimens were obtained from a testicular DLBCL occurring in a 64-year-old man. The cytological features of imprints were compared with the results obtained from histological and immunohistochemical examinations. Smears obtained from the touch imprints exhibited a high cellular yield consisting of discretely arranged monomorphic large cells with irregular nuclear membranes, scant cytoplasm and conspicuous nucleoli. Histologically, the tumor consisted of discohesive neoplastic lymphocytes that infiltrated diffusely and produced a wide separation of intact seminiferous tubules. Diffuse, intense immunostaining for CD45, CD20, MUM1 and Ki-67 led to the diagnosis of primary DLBCL of the testis with a non-germinal center B-cell-like phenotype. Careful observation of the touch imprint specimens of testicular DLBCL revealed a high cellularity with a predominant single-cell pattern of monomorphic cells demonstrating irregular nuclear membranes and conspicuous nucleoli. In addition, DLBCL is capable of developing in the testis and forming a predominantly discohesive cell population, suggesting the presence of a lymphoid malignancy. Thus, it may be possible to detect morphological features that are characteristic of DLBCL using imprint cytology. To the best of our knowledge, this is the first study reporting the diagnosis of testicular DLBCL using touch imprint cytology.

View Figures

View References

1.	Horne MJ and Adeniran AJ: Primary diffuse large B-cell lymphoma of the testis. Arch Pathol Lab Med. 135:1363–1367. 2011. View Article : Google Scholar : PubMed/NCBI
2.	Shahab N and Doll DC: Testicular lymphoma. Semin Oncol. 26:259–269. 1999.
3.	Miedler JD and MacLennan GT: Primary testicular lymphoma. J Urol. 178:26452007. View Article : Google Scholar : PubMed/NCBI
4.	Doll DC and Weiss RB: Malignant lymphoma of the testis. Am J Med. 81:515–524. 1986. View Article : Google Scholar : PubMed/NCBI
5.	Zucca E, Conconi A, Mughal TI, et al International Extranodal Lymphoma Study Group: Patterns of outcome and prognostic factors in primary large-cell lymphoma of the testis in a survey by the International Extranodal Lymphoma Study Group. J Clin Oncol. 21:20–27. 2003. View Article : Google Scholar : PubMed/NCBI
6.	Li D, Xie P and Mi C: Primary testicular diffuse large B-cell lymphoma shows an activated B-cell-like phenotype. Pathol Res Pract. 206:611–615. 2010. View Article : Google Scholar : PubMed/NCBI
7.	Alizadeh AA, Eisen MB, Davis RE, et al: Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature. 403:503–511. 2000. View Article : Google Scholar : PubMed/NCBI
8.	Rammou-Kinia R, Anagnostopoulou I, Tassiopoulos F and Lykourinas M: Fine needle aspiration of the testis. Correlation between cytology and histology. Acta Cytol. 43:991–998. 1999. View Article : Google Scholar : PubMed/NCBI
9.	Verma K, Ram TR and Kapila K: Value of fine needle aspiration cytology in the diagnosis of testicular neoplasms. Acta Cytol. 33:631–634. 1989.PubMed/NCBI
10.	Pandey A, Nandini N, Jha A and Manjunath G: Fine needle aspiration cytology and cell block in the diagnosis of seminoma testis. J Cytol. 28:39–41. 2011. View Article : Google Scholar : PubMed/NCBI
11.	Saran RK, Banerjee AK, Gupta SK and Rajwanshi A: Spermatocytic seminoma: a cytology and histology case report with review of the literature. Diagn Cytopathol. 20:233–236. 1999. View Article : Google Scholar : PubMed/NCBI
12.	Das DK: Value and limitations of fine-needle aspiration cytology in diagnosis and classification of lymphomas: A review. Diagn Cytopathol. 21:240–249. 1999. View Article : Google Scholar : PubMed/NCBI
13.	Hans CP, Weisenburger DD, Greiner TC, et al: Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray. Blood. 103:275–282. 2004. View Article : Google Scholar : PubMed/NCBI
14.	Al-Abbadi MA, Hattab EM, Tarawneh MS, Amr SS, Orazi A and Ulbright TM: Primary testicular diffuse large B-cell lymphoma belongs to the nongerminal center B-cell-like subgroup: a study of 18 cases. Mod Pathol. 19:1521–1527. 2006. View Article : Google Scholar : PubMed/NCBI
15.	Vitolo U, Ferreri AJ and Zucca E: Primary testicular lymphoma. Crit Rev Oncol Hematol. 65:183–189. 2008. View Article : Google Scholar
16.	Coiffier B: Rituximab therapy in malignant lymphoma. Oncogene. 26:3603–3613. 2007. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Primary testicular diffuse large B-cell lymphoma: A case report focusing on touch imprint cytology and a non-germinal center B-cell-like phenotype

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles