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Article

Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4

  • Authors:
    • Xiaoyin Wang
    • Junhe Zhang
    • Yuxin Yang
    • Weihua Dong
    • Fang Wang
    • Li Wang
    • Xiaojuan Li
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Ophthalmology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
  • Pages: 263-267
    |
    Published online on: May 15, 2013
       https://doi.org/10.3892/etm.2013.1116
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Abstract

The aim of this study was to investigate the effects of progesterone (PROG) on blood-brain barrier (BBB) permeability, cerebral edema and the expression of matrix metalloproteinase-9 (MMP-9) and aquaporin-4 (AQP-4) in neonatal rats with hypoxic-ischemic brain damage (HIBD) and to explore the mechanism of its neuroprotective effect. Sixty 7-day-old Wistar rats were divided into sham surgery, hypoxic ischemia (HI) and drug prophylaxis (PROG) groups. HIBD animal models were established. All the animals were sacrificed after 24 h. The BBB was assessed using Evans blue. Cerebral moisture capacity was determined using the dry-wet method. MMP-9 was detected in the brain tissues using enzyme-linked immunosorbent assay. The expression of AQP-4 and MMP-9 in the cerebral cortex was observed using immunohistochemistry and real-time polymerase chain reaction. The MMP-9 levels in the cortex, BBB permeability, cerebral moisture capacity and expression of AQP-4 and MMP-9 in the HI group were significantly higher compared with those in the sham surgery group (P<0.01), and they were significantly lower in the drug prophylaxis group compared with those in the HI group (P<0.05). In conclusion, PROG reduces BBB damage and cerebral edema and inhibits MMP-9 generation to protect rat brains against HIBD. The protective effect of PROG may be correlated with downregulated expression of AQP-4 and MMP-9 in the cerebral cortex.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Zhang J, Yang Y, Dong W, Wang F, Wang L and Li X: Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4. Exp Ther Med 6: 263-267, 2013.
APA
Wang, X., Zhang, J., Yang, Y., Dong, W., Wang, F., Wang, L., & Li, X. (2013). Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4. Experimental and Therapeutic Medicine, 6, 263-267. https://doi.org/10.3892/etm.2013.1116
MLA
Wang, X., Zhang, J., Yang, Y., Dong, W., Wang, F., Wang, L., Li, X."Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4". Experimental and Therapeutic Medicine 6.1 (2013): 263-267.
Chicago
Wang, X., Zhang, J., Yang, Y., Dong, W., Wang, F., Wang, L., Li, X."Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4". Experimental and Therapeutic Medicine 6, no. 1 (2013): 263-267. https://doi.org/10.3892/etm.2013.1116
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Zhang J, Yang Y, Dong W, Wang F, Wang L and Li X: Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4. Exp Ther Med 6: 263-267, 2013.
APA
Wang, X., Zhang, J., Yang, Y., Dong, W., Wang, F., Wang, L., & Li, X. (2013). Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4. Experimental and Therapeutic Medicine, 6, 263-267. https://doi.org/10.3892/etm.2013.1116
MLA
Wang, X., Zhang, J., Yang, Y., Dong, W., Wang, F., Wang, L., Li, X."Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4". Experimental and Therapeutic Medicine 6.1 (2013): 263-267.
Chicago
Wang, X., Zhang, J., Yang, Y., Dong, W., Wang, F., Wang, L., Li, X."Progesterone attenuates cerebral edema in neonatal rats with hypoxic-ischemic brain damage by inhibiting the expression of matrix metalloproteinase-9 and aquaporin-4". Experimental and Therapeutic Medicine 6, no. 1 (2013): 263-267. https://doi.org/10.3892/etm.2013.1116
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