Effect of Lycopersicon esculentum extract on apoptosis in the rat cerebellum, following prenatal and postnatal exposure to an electromagnetic field

Köktürk,Sibel; Yardimoglu,Melda; Celikozlu,Saadet  D.; Dolanbay,Elif  Gelenli; Cimbiz,Ali

doi:10.3892/etm.2013.1123

Effect of Lycopersicon esculentum extract on apoptosis in the rat cerebellum, following prenatal and postnatal exposure to an electromagnetic field

Authors:
- Sibel Köktürk
- Melda Yardimoglu
- Saadet D. Celikozlu
- Elif Gelenli Dolanbay
- Ali Cimbiz
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Affiliations: Department of Histology and Embryology, Faculty of Medicine, Ordu University, Ordu 52200, Turkey, Department of Histology and Embryology, Faculty of Medicine, Kocaeli University, Kocaeli 41380, Turkey, Altıntaş Vocational School of Dumlupinar University, Altıntaş, Kütahya 43800, Turkey, Department of Physical Therapy and Rehabilitation, Faculty of Health Sciences, Zirve University, Gaziantep 27260, Turkey
Published online on: May 17, 2013 https://doi.org/10.3892/etm.2013.1123
Pages: 52-56

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Abstract

The expansion of mobile phone technology has raised concerns regarding the effect of 900‑MHz electromagnetic field (EMF) exposure on the central nervous system. At present, the developing human brain is regularly exposed to mobile telephones, pre‑ and postnatally. Several studies have demonstrated the acute effects of EMF exposure during pre‑ or postnatal periods; however, the chronic effects of EMF exposure are less understood. Thus, the aim of the present study was to determine the chronic effects of EMF on the pre‑ and postnatal rat cerebellum. The control group was maintained in the same conditions as the experimental groups, without the exposure to EMF. In the EMF1 group, the rats were exposed to EMF during pre‑ and postnatal periods (until postnatal day 80). In the EMF2 group, the rats were also exposed to EMF pre‑ and postnatally; in addition, however, they were provided with a daily oral supplementation of Lycopersicon esculentum extract (~2 g/kg). The number of caspase‑3‑labeled Purkinje neurons and granule cells present in the rats in the control and experimental groups were then counted. The neurodegenerative changes were studied using cresyl violet staining, and these changes were evaluated. In comparison with the control animals, the EMF1 group demonstrated a significant increase in the number of caspase‑3‑labeled Purkinje neurons and granule cells present in the cerebellum (P<0.001). However, in comparison with the EMF1 group, the EMF2 group exhibited significantly fewer caspase‑3‑labeled Purkinje neurons and granule cells in the cerebellum. In the EMF1 group, the Purkinje neurons were revealed to have undergone dark neuron degenerative changes. However, the presence of dark Purkinje neurons was reduced in the EMF2 group, compared with the EMF1 group. The results indicated that apoptosis and neurodegeneration in rats exposed to EMF during pre‑ and postnatal periods may be reduced with Lycopersicon esculentum extract therapy.

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1.	Akdag MZ, Dasdag S, Ulukaya E, Uzunlar AK, Kurt MA and Taşkin A: Effects of extremely low-frequency magnetic field on caspase activities and oxidative stress values in rat brain. Biol Trace Elem Res. 138:238–249. 2010. View Article : Google Scholar : PubMed/NCBI
2.	Kim TH, Huang TQ, Jang JJ, et al: Local exposure of 849 MHz and 1763 MHz radiofrequency radiation to mouse heads does not induce cell death or cell proliferation in brain. Exp Mol Med. 40:294–303. 2008. View Article : Google Scholar : PubMed/NCBI
3.	Joubert V, Leveque P, Cueille M, Bourthoumieu S and Yardin C: No apoptosis is induced in rat cortical neurons exposed to GSM phone fields. Bioelectromagnetics. 28:115–121. 2007. View Article : Google Scholar : PubMed/NCBI
4.	McNamee JP, Bellier PV, McLean JR, Marro L, Gajda GB and Thansandote A: DNA damage and apoptosis in the immature mouse cerebellum after acute exposure to a 1 mT, 60 Hz magnetic field. Mutat Res. 513:121–133. 2002. View Article : Google Scholar : PubMed/NCBI
5.	Lai H and Singh NP: Magnetic-field-induced DNA strand breaks in brain cells of the rat. Environ Health Perspect. 112:687–694. 2004. View Article : Google Scholar : PubMed/NCBI
6.	Grassi C, D’Ascenzo M, Torsello A, Martinotti G, Wolf F, Cittadini A and Azzena GB: Effects of 50 Hz electromagnetic fields on voltage-gated Ca2+ channels and their role in modulation of neuroendocrine cell proliferation and death. Cell Calcium. 35:307–315. 2004.
7.	Maskey D, Pradhan J, Aryal B, et al: Chronic 835-MHz radio-frequency exposure to mice hippocampus alters the distribution of calbindin and GFAP immunoreactivity. Brain Res. 1346:237–246. 2010. View Article : Google Scholar : PubMed/NCBI
8.	Sonmez OF, Odaci E, Bas O and Kaplan S: Purkinje cell number decreases in the adult female rat cerebellum following exposure to 900 MHz electromagnetic field. Brain Res. 1356:95–101. 2010. View Article : Google Scholar : PubMed/NCBI
9.	MacKenzie SH and Clark AC: Death by caspase dimerization. Adv Exp Med Biol. 747:55–73. 2012. View Article : Google Scholar : PubMed/NCBI
10.	Wickman G, Julian L and Olson MF: How apoptotic cells aid in the removal of their own cold dead bodies. Cell Death Differ. 19:735–742. 2012. View Article : Google Scholar : PubMed/NCBI
11.	Folch J, Alvira D, López-Querol M, et al: Evaluation of transcriptional activity of caspase-3 gene as a marker of acute neurotoxicity in rat cerebellar granular cells. Toxicol In Vitro. 24:465–471. 2010. View Article : Google Scholar : PubMed/NCBI
12.	Snigdha S, Smith ED, Prieto GA, et al: Caspase-3 activation as a bifurcation point between plasticity and cell death. Neurosci Bull. 28:14–24. 2012. View Article : Google Scholar : PubMed/NCBI
13.	Pace V, Bellizzi D, Giordano F, et al: Experimental testing of a mathematical model relevant to the extrinsic pathway of apoptosis. Cell Stress Chaperones. 15:13–23. 2010. View Article : Google Scholar : PubMed/NCBI
14.	Duran-Vilaregut J, Del Valle J, Manich G, et al: Systemic administration of 3-nitropropionic acid points out a different role for active caspase-3 in neurons and astrocytes. Neurochem Int. 56:443–450. 2010. View Article : Google Scholar : PubMed/NCBI
15.	Hwang IK, Ahn JH, Yoo DY, et al: Increased immunoreactivities of cleaved αII-spectrin and cleaved caspase-3 in the aged dog spinal cord. Neurochem Res. 37:480–486. 2012.
16.	Liu J, Lu Y and Liang J: A novel fluorescence derivatization method combined with HPLC for determining the activities of endogenous caspase. Analyst. 137:5097–5104. 2012. View Article : Google Scholar : PubMed/NCBI
17.	Hossmann KA: Effects of electromagnetic radiation of mobile phones on the central nervous system. Bioelectromagnetics. 24:49–62. 2003. View Article : Google Scholar : PubMed/NCBI
18.	The sensitivity of children to EMF exposure. In: Proceedings of the 2004 EPRI-Cosponsored World Health Organization Workshop; EPRI, Palo Alto, NC, USA. 1011147(3): pp. 3–13. 2004
19.	Rodier PM: Chronology of neuron development: animal studies and their clinical implications. Dev Med Child Neurol. 22:525–545. 1980. View Article : Google Scholar : PubMed/NCBI
20.	Bas O, Odaci E, Kaplan S, et al: 900 MHz electromagnetic field exposure affects qualitative and quantitative features of hippocampal pyramidal cells in the adult female rat. Brain Res. 1265:178–185. 2009. View Article : Google Scholar : PubMed/NCBI
21.	Giovannucci E: Tomato products, lycopene, and prostate cancer: a review of the epidemiological literature. J Nutr. 135:2030S–2031S. 2005.PubMed/NCBI
22.	Ford NA, Elsen AC, Zuniga K, Lindshield BL and Erdman JW Jr: Lycopene and apo-12′-lycopenal reduce cell proliferation and alter cell cycle progression in human prostate cancer cells. Nutr Cancer. 63:256–263. 2011.PubMed/NCBI
23.	Teodoro AJ, Oliveira FL, Martins NB, Maia Gde A, Martucci RB and Borojevic R: Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines. Cancer Cell Int. 12:362012. View Article : Google Scholar : PubMed/NCBI
24.	Rissanen TH, Voutilainen S, Nyyssönen K, Salonen R, Kaplan GA and Salonen JT: Serum lycopene concentrations and carotid atherosclerosis: the Kuopio Ischaemic Heart Disease Risk Factor Study. Am J Clin Nutr. 77:133–138. 2003.PubMed/NCBI
25.	Coyne T, Ibiebele TI, Baade PD, Dobson A, McClintock C, Dunn S, et al: Diabetes mellitus and serum carotenoids: findings of a population-based study in Queensland, Australia. Am J Clin Nutr. 82:685–693. 2005.PubMed/NCBI
26.	Agca CA, Tuzcu M, Gencoglu H, Akdemir F, Ali S, Sahin K and Kucuk O: Lycopene counteracts the hepatic response to 7,12-dimethylbenz[a]anthracene by altering the expression of Bax, Bcl-2, caspases, and oxidative stress biomarkers. Pharm Biol. 50:1513–1518. 2012.PubMed/NCBI
27.	Kelkel M, Schumacher M and Dicato M: Antioxidant and anti-proliferative properties of lycopene. Free Radic Res. 45:925–940. 2011. View Article : Google Scholar : PubMed/NCBI
28.	Kong KW, Rajab NF, Prasad KN, Ismail A, Markom M and Tan CP: Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. Food Chem. 123:1142–1148. 2010. View Article : Google Scholar
29.	Khachik F, Carvalho L, Bernstein PS, Muir GJ, Zhao DY and Katz NB: Chemistry, distribution, and metabolism of tomato carotenoids and their impact on human health. Exp Biol Med (Maywood). 227:845–851. 2002.PubMed/NCBI
30.	Qu M, Zhou Z, Chen C, et al: Lycopene protects against trimethyltin-induced neurotoxicity in primary cultured rat hippocampal neurons by inhibiting the mitochondrial apoptotic pathway. Neurochem Int. 59:1095–1103. 2011. View Article : Google Scholar : PubMed/NCBI
31.	Maeda K, Maeda T and Qi Y: In vitro and in vivo induction of human LoVo cells into apoptotic process by non-invasive microwave treatment: A potentially novel approach for physical therapy of human colorectal cancer. Oncol Rep. 11:771–775. 2004.
32.	Caraglia M, Marra M, Mancinelli F, et al: Electromagnetic fields at mobile phone frequency induce apoptosis and inactivation of the multi-chaperone complex in human epidermoid cancer cells. J Cell Physiol. 204:539–548. 2005. View Article : Google Scholar
33.	Capri M, Scarcella E, Bianchi E, et al: 1800 MHz radiofrequency (mobile phones, different Global System for Mobile communication modulations) does not affect apoptosis and heat shock protein 70 level in peripheral blood mononuclear cells from young and old donors. Int J Radiat Biol. 80:389–397. 2004. View Article : Google Scholar
34.	Capri M, Scarcella E, Fumelli C, et al: In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radio-frequency: studies of proliferation, apoptosis and mitochondrial membrane potential. Radiat Res. 162:211–218. 2004. View Article : Google Scholar
35.	Aydın S, Tokaç M, Taner G, et al: Antioxidant and antigenotoxic effects of lycopene in obstructive jaundice. J Surg Res. Nov 7–2012.(Epub ahead of print). View Article : Google Scholar
36.	Saada HN, Rezk RG and Eltahawy NA: Lycopene protects the structure of the small intestine against gamma-radiation-induced oxidative stress. Phytother Res. 24(Suppl 2): S204–S208. 2010. View Article : Google Scholar : PubMed/NCBI
37.	Tang Y, Parmakhtiar B, Simoneau AR, Xie J, Fruehauf J, Lilly M and Zi X: Lycopene enhances docetaxel’s effect in castration-resistant prostate cancer associated with insulin-like growth factor I receptor levels. Neoplasia. 13:108–119. 2011.PubMed/NCBI
38.	Gervais FG, Xu D, Robertson GS, et al: Involvement of caspases in proteolytic cleavage of Alzheimer’s amyloid-beta precursor protein and amyloidogenic A beta peptide formation. Cell. 97:395–406. 1999.
39.	Türk G, Ceribaşi AO, Sakin F, Sönmez M and Ateşşahin A: Antiperoxidative and anti-apoptotic effects of lycopene and ellagic acid on cyclophosphamide-induced testicular lipid peroxidation and apoptosis. Reprod Fertil Dev. 22:587–596. 2010.PubMed/NCBI