Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August 2013 Volume 6 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August 2013 Volume 6 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of herba centellae on the expression of HGF and MCP-1

  • Authors:
    • Zhu Zhang
    • Guangce Wang
    • Jiwei Ma
    • Haofei Liu
    • Xitao Zhang
    • Guangling Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Nephropathy, The First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450000, P.R. China
  • Pages: 427-434
    |
    Published online on: June 6, 2013
       https://doi.org/10.3892/etm.2013.1146
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to explore the effects of herba centellae on protein and mRNA expression of hepatocyte growth factor (HGF), and monocyte chemotactic protein-1 (MCP-1) in renal tubulointerstitial fibrosis (TIF). A unilateral ureteral obstruction (UUO) model was established in 50 male Sprague Dawley (SD) rats. Blood samples were collected and the blood urea nitrogen (BUN) levels, serum creatinine (Scr), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured. Immunohistochemistry (IHC) detected the localization and expression levels of HGF and MCP-1. In addition, quantitative polymerase chain reaction (qPCR) detected the mRNA expression of HGF and MCP-1. Thirty rats were used to prepare the rat serum containing drug by cell culture, and qPCR and immunocytochemistry (ICC) were performed to examine the mRNA and protein expression of HGF and MCP-1. MCP-1 and its mRNA expression was significantly higher in rat renal interstitium of the UUO group and cells of the transforming growth factor‑β1 (TGF‑β1) stimulation group compared with that of the control group (P<0.01). MCP‑1 and its mRNA expression in the drug intervention group were significantly reduced compared with that of the UUO model group (P<0.01). However, MCP‑1 and its mRNA expression in the high-dose herba centellae group was significantly lower compared with that of the low-dose herba centellae group (P<0.01). Furthermore, HGF and its mRNA expression significantly increased in the drug intervention group (P<0.01), and expression in the high-dose group was significantly higher compared with that of the low-dose group (P<0.01), but similar to that of the fosinopril group (P>0.05). The levels of BUN decreased in the drug intervention group; however, no significant differences were determined in Scr, ALT and AST levels. Herba centellae may have inhibited MCP‑1 and its mRNA expression through the upregulation of HGF and its mRNA expression, in order to achieve resistance to TIF without showing evident hepatorenal toxicity.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1. 

Eddy AA: Molecular basis of renal fibrosis. Pediatr Nephrol. 15:290–301. 2000. View Article : Google Scholar

2. 

Klahr S and Morrissey J: Obstructive nephropathy and renal fibrosis. Am J Physiol Renal Physiol. 283:F861–F875. 2002. View Article : Google Scholar : PubMed/NCBI

3. 

Kobayashi E, Sasamura H, Mifune M, et al: Hepatocyte growth factor regulates proteoglycan synthesis in interstitial fibroblasts. Kidney Int. 64:1179–1788. 2003. View Article : Google Scholar : PubMed/NCBI

4. 

Iwano M, Plieth D, Danoff TM, Xue C, Okada H and Neilson EG: Evidence that fibroblasts derive from epithelium during tissue fibrosis. J Clin lnvest. 11:341–350. 2002. View Article : Google Scholar : PubMed/NCBI

5. 

Wada T, Furuichi K, Sakai N, et al: Gene therapy via blockade of monocyte chemoattractant protein-1 for renal fibrosis. J Am Soc Nephrol. 15:940–948. 2004. View Article : Google Scholar : PubMed/NCBI

6. 

Liu Y: Epithelial to mesenchymal transition in renal fibrogenesis: pathologic significance, molecular mechanism, and therapeutic intervention. J Am Soc Nephrol. 15:1–12. 2004. View Article : Google Scholar

7. 

Zhang Z, Wan JY, Luo FL, Li HZ and Zhou QX: Studies on analgesic effect and mechanism of asiaticoside on lipopolysaccharide induced hyperalgesia in mice. Clin Pharm J. 43:751–753. 2008.

8. 

Liu ZF, Zhao HN and Nie SL: Progress in the research on the mechanism of the pharmacological effects of asiaticoside. Guangdong Med J. 30:649–651. 2009.

9. 

Dadfar E, Lundahl J and Jacobson SH: Monocyte adhesion molecule expression in interstitial inflammation in patients with renal failure. Nephrol Dial Transplant. 19:614–622. 2004. View Article : Google Scholar : PubMed/NCBI

10. 

Ranieri E, Gesualdo L, Petrarulo F and Schena FP: Urinary IL-6/EGF ratio: a useful prognostic marker for the progression of renal damage in IgA nephropathy. Kidney Int. 50:1990–2001. 1996. View Article : Google Scholar : PubMed/NCBI

11. 

Chevalier RL, Forbes MS and Thornhill BA: Ureteral obstruction as a model of renal interstitial fibrosis and obstructive nephropathy. Kidney Int. 75:1145–1152. 2009. View Article : Google Scholar : PubMed/NCBI

12. 

Bohle A, Müller GA, Wehrmann M, Mackensen-Haen S and Xiao JC: Pathogenesis of chronic renal failure in the primary glomerulopathies, renal vasculopathies and chronic interstitial nephritides. Kidney Int Suppl. 54:S2–S9. 1996.PubMed/NCBI

13. 

Zeisberg M, Maeshima Y, Mosterman B and Kalluri R: Renal fibrosis: extracellular matrix microenvironment regulates migratory behavior of activated tubular epithelial cells. Am J Pathol. 160:2001–2008. 2002. View Article : Google Scholar

14. 

Okada H and Kalluri R: Cellular and molecular pathways that lead to progression and regression of renal fibrogenesis. Curr Mol Med. 5:467–474. 2005. View Article : Google Scholar : PubMed/NCBI

15. 

Kroening S, Solomovitch S, Sachs M, Wullich B and Goppelt-Struebe M: Regulation of connective tissue growth factor (CTGF) by hepatocyte growth factor in human tubular epithelial cells. Nephrol Dial Transplant. 24:755–762. 2009. View Article : Google Scholar : PubMed/NCBI

16. 

Yang J, Dai C and Liu Y: A novel mechanism by which hepatocyte growth factor blocks tubular epithelial to mesenchymal transition. J Am Soc Nephrol. 16:68–78. 2005. View Article : Google Scholar : PubMed/NCBI

17. 

Yang J, Dai C and Liu Y: Hepatocyte growth factor suppresses renal interstitial myofibroblast activation and intercepts Smad signal transduction. Am J Pathol. 163:621–632. 2003. View Article : Google Scholar : PubMed/NCBI

18. 

Kaschina E and Unger T: Pathophysiologic link between atherosclerosis and nephrosclerosis. Cardiorenal Syndrome. 5:245–253. 2010. View Article : Google Scholar

19. 

Panzer U, Thaiss F, Zahner G, et al: Monocyte chemoattractant protein-1 and osteopontin differentially regulate monocytes recruitment in experimental glomerulonephritis. Kidney Int. 59:1762–1769. 2001. View Article : Google Scholar

20. 

Huang YY, Xu AP, Zhou SS, Fu JZ and Du H: Effect of losartan on renal expression of monocyte chemoattractant protein-1 and transforming growth factor-β(1) in rats after unilateral ureteral obstruction. J South Med Univ. 31:1405–1410. 2011.

21. 

Zhang Z, Zhao L, Wang B, et al: Effects of Centella asiatica on expression of connective colledge tissue growth factor in UUO rats. Chin J Integr Tradit Western Nephrol. 9:118–120. 2008.

22. 

Zhang Z, Wang SG, Wang B, et al: Effects of Centella asiatica Granule on renal tissue in rats with unilateral ureteral obstruction alpha-smooth muscle actin expression. Tradit Chin Med Res. 22:15–18. 2009.

23. 

Wang LL, Liu PN, Ma JW, et al: Effect of Centella asiatica granula on TGF-β1-induced expression of bone morphogenetic protein-7 in renal tubular epithelial cells. Shandong Med J. 49:13–15. 2009.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Wang G, Ma J, Liu H, Zhang X and Zhu G: Effect of herba centellae on the expression of HGF and MCP-1. Exp Ther Med 6: 427-434, 2013.
APA
Zhang, Z., Wang, G., Ma, J., Liu, H., Zhang, X., & Zhu, G. (2013). Effect of herba centellae on the expression of HGF and MCP-1. Experimental and Therapeutic Medicine, 6, 427-434. https://doi.org/10.3892/etm.2013.1146
MLA
Zhang, Z., Wang, G., Ma, J., Liu, H., Zhang, X., Zhu, G."Effect of herba centellae on the expression of HGF and MCP-1". Experimental and Therapeutic Medicine 6.2 (2013): 427-434.
Chicago
Zhang, Z., Wang, G., Ma, J., Liu, H., Zhang, X., Zhu, G."Effect of herba centellae on the expression of HGF and MCP-1". Experimental and Therapeutic Medicine 6, no. 2 (2013): 427-434. https://doi.org/10.3892/etm.2013.1146
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Wang G, Ma J, Liu H, Zhang X and Zhu G: Effect of herba centellae on the expression of HGF and MCP-1. Exp Ther Med 6: 427-434, 2013.
APA
Zhang, Z., Wang, G., Ma, J., Liu, H., Zhang, X., & Zhu, G. (2013). Effect of herba centellae on the expression of HGF and MCP-1. Experimental and Therapeutic Medicine, 6, 427-434. https://doi.org/10.3892/etm.2013.1146
MLA
Zhang, Z., Wang, G., Ma, J., Liu, H., Zhang, X., Zhu, G."Effect of herba centellae on the expression of HGF and MCP-1". Experimental and Therapeutic Medicine 6.2 (2013): 427-434.
Chicago
Zhang, Z., Wang, G., Ma, J., Liu, H., Zhang, X., Zhu, G."Effect of herba centellae on the expression of HGF and MCP-1". Experimental and Therapeutic Medicine 6, no. 2 (2013): 427-434. https://doi.org/10.3892/etm.2013.1146
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team