Effect of herba centellae on the expression of HGF and MCP-1

Zhang,Zhu; Wang,Guangce; Ma,Jiwei; Liu,Haofei; Zhang,Xitao; Zhu,Guangling

doi:10.3892/etm.2013.1146

Effect of herba centellae on the expression of HGF and MCP-1

Authors:
- Zhu Zhang
- Guangce Wang
- Jiwei Ma
- Haofei Liu
- Xitao Zhang
- Guangling Zhu
View Affiliations

Affiliations: Department of Nephropathy, The First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450000, P.R. China
Published online on: June 6, 2013 https://doi.org/10.3892/etm.2013.1146
Pages: 427-434

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to explore the effects of herba centellae on protein and mRNA expression of hepatocyte growth factor (HGF), and monocyte chemotactic protein-1 (MCP-1) in renal tubulointerstitial fibrosis (TIF). A unilateral ureteral obstruction (UUO) model was established in 50 male Sprague Dawley (SD) rats. Blood samples were collected and the blood urea nitrogen (BUN) levels, serum creatinine (Scr), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured. Immunohistochemistry (IHC) detected the localization and expression levels of HGF and MCP-1. In addition, quantitative polymerase chain reaction (qPCR) detected the mRNA expression of HGF and MCP-1. Thirty rats were used to prepare the rat serum containing drug by cell culture, and qPCR and immunocytochemistry (ICC) were performed to examine the mRNA and protein expression of HGF and MCP-1. MCP-1 and its mRNA expression was significantly higher in rat renal interstitium of the UUO group and cells of the transforming growth factor‑β1 (TGF‑β1) stimulation group compared with that of the control group (P<0.01). MCP‑1 and its mRNA expression in the drug intervention group were significantly reduced compared with that of the UUO model group (P<0.01). However, MCP‑1 and its mRNA expression in the high-dose herba centellae group was significantly lower compared with that of the low-dose herba centellae group (P<0.01). Furthermore, HGF and its mRNA expression significantly increased in the drug intervention group (P<0.01), and expression in the high-dose group was significantly higher compared with that of the low-dose group (P<0.01), but similar to that of the fosinopril group (P>0.05). The levels of BUN decreased in the drug intervention group; however, no significant differences were determined in Scr, ALT and AST levels. Herba centellae may have inhibited MCP‑1 and its mRNA expression through the upregulation of HGF and its mRNA expression, in order to achieve resistance to TIF without showing evident hepatorenal toxicity.

View Figures

View References

1.	Eddy AA: Molecular basis of renal fibrosis. Pediatr Nephrol. 15:290–301. 2000. View Article : Google Scholar
2.	Klahr S and Morrissey J: Obstructive nephropathy and renal fibrosis. Am J Physiol Renal Physiol. 283:F861–F875. 2002. View Article : Google Scholar : PubMed/NCBI
3.	Kobayashi E, Sasamura H, Mifune M, et al: Hepatocyte growth factor regulates proteoglycan synthesis in interstitial fibroblasts. Kidney Int. 64:1179–1788. 2003. View Article : Google Scholar : PubMed/NCBI
4.	Iwano M, Plieth D, Danoff TM, Xue C, Okada H and Neilson EG: Evidence that fibroblasts derive from epithelium during tissue fibrosis. J Clin lnvest. 11:341–350. 2002. View Article : Google Scholar : PubMed/NCBI
5.	Wada T, Furuichi K, Sakai N, et al: Gene therapy via blockade of monocyte chemoattractant protein-1 for renal fibrosis. J Am Soc Nephrol. 15:940–948. 2004. View Article : Google Scholar : PubMed/NCBI
6.	Liu Y: Epithelial to mesenchymal transition in renal fibrogenesis: pathologic significance, molecular mechanism, and therapeutic intervention. J Am Soc Nephrol. 15:1–12. 2004. View Article : Google Scholar
7.	Zhang Z, Wan JY, Luo FL, Li HZ and Zhou QX: Studies on analgesic effect and mechanism of asiaticoside on lipopolysaccharide induced hyperalgesia in mice. Clin Pharm J. 43:751–753. 2008.
8.	Liu ZF, Zhao HN and Nie SL: Progress in the research on the mechanism of the pharmacological effects of asiaticoside. Guangdong Med J. 30:649–651. 2009.
9.	Dadfar E, Lundahl J and Jacobson SH: Monocyte adhesion molecule expression in interstitial inflammation in patients with renal failure. Nephrol Dial Transplant. 19:614–622. 2004. View Article : Google Scholar : PubMed/NCBI
10.	Ranieri E, Gesualdo L, Petrarulo F and Schena FP: Urinary IL-6/EGF ratio: a useful prognostic marker for the progression of renal damage in IgA nephropathy. Kidney Int. 50:1990–2001. 1996. View Article : Google Scholar : PubMed/NCBI
11.	Chevalier RL, Forbes MS and Thornhill BA: Ureteral obstruction as a model of renal interstitial fibrosis and obstructive nephropathy. Kidney Int. 75:1145–1152. 2009. View Article : Google Scholar : PubMed/NCBI
12.	Bohle A, Müller GA, Wehrmann M, Mackensen-Haen S and Xiao JC: Pathogenesis of chronic renal failure in the primary glomerulopathies, renal vasculopathies and chronic interstitial nephritides. Kidney Int Suppl. 54:S2–S9. 1996.PubMed/NCBI
13.	Zeisberg M, Maeshima Y, Mosterman B and Kalluri R: Renal fibrosis: extracellular matrix microenvironment regulates migratory behavior of activated tubular epithelial cells. Am J Pathol. 160:2001–2008. 2002. View Article : Google Scholar
14.	Okada H and Kalluri R: Cellular and molecular pathways that lead to progression and regression of renal fibrogenesis. Curr Mol Med. 5:467–474. 2005. View Article : Google Scholar : PubMed/NCBI
15.	Kroening S, Solomovitch S, Sachs M, Wullich B and Goppelt-Struebe M: Regulation of connective tissue growth factor (CTGF) by hepatocyte growth factor in human tubular epithelial cells. Nephrol Dial Transplant. 24:755–762. 2009. View Article : Google Scholar : PubMed/NCBI
16.	Yang J, Dai C and Liu Y: A novel mechanism by which hepatocyte growth factor blocks tubular epithelial to mesenchymal transition. J Am Soc Nephrol. 16:68–78. 2005. View Article : Google Scholar : PubMed/NCBI
17.	Yang J, Dai C and Liu Y: Hepatocyte growth factor suppresses renal interstitial myofibroblast activation and intercepts Smad signal transduction. Am J Pathol. 163:621–632. 2003. View Article : Google Scholar : PubMed/NCBI
18.	Kaschina E and Unger T: Pathophysiologic link between atherosclerosis and nephrosclerosis. Cardiorenal Syndrome. 5:245–253. 2010. View Article : Google Scholar
19.	Panzer U, Thaiss F, Zahner G, et al: Monocyte chemoattractant protein-1 and osteopontin differentially regulate monocytes recruitment in experimental glomerulonephritis. Kidney Int. 59:1762–1769. 2001. View Article : Google Scholar
20.	Huang YY, Xu AP, Zhou SS, Fu JZ and Du H: Effect of losartan on renal expression of monocyte chemoattractant protein-1 and transforming growth factor-β(1) in rats after unilateral ureteral obstruction. J South Med Univ. 31:1405–1410. 2011.
21.	Zhang Z, Zhao L, Wang B, et al: Effects of Centella asiatica on expression of connective colledge tissue growth factor in UUO rats. Chin J Integr Tradit Western Nephrol. 9:118–120. 2008.
22.	Zhang Z, Wang SG, Wang B, et al: Effects of Centella asiatica Granule on renal tissue in rats with unilateral ureteral obstruction alpha-smooth muscle actin expression. Tradit Chin Med Res. 22:15–18. 2009.
23.	Wang LL, Liu PN, Ma JW, et al: Effect of Centella asiatica granula on TGF-β1-induced expression of bone morphogenetic protein-7 in renal tubular epithelial cells. Shandong Med J. 49:13–15. 2009.