Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August 2013 Volume 6 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August 2013 Volume 6 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice

  • Authors:
    • Xia Yue
    • Hongsheng Yu
    • Xialu Lin
    • Kui Liu
    • Xin Wang
    • Fei Zhou
    • Jinshun Zhao
    • Baobo Zou
  • View Affiliations / Copyright

    Affiliations: Medical School and Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo University, Ningbo, Zhejiang 315211, P.R. China
  • Pages: 341-346
    |
    Published online on: June 13, 2013
       https://doi.org/10.3892/etm.2013.1154
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In the present study, the left anterior descending coronary arteries of mice under anesthesia were ligated, and the optimal surgical conditions for coronary artery ligation (CAL) in the establishment of a myocardial infarction (MI) mouse model were investigated. All mice that survived were sacrificed seven days subsequent to the successful surgery. Body weight, blood serum and heart tissues were obtained for further analysis or biochemical and histopathological examinations. The survival rate of the mice following the CAL procedure was 70%. The aspartate aminotransferase (AST), creatine kinase (CK) and lactate dehydrogenase (LDH) concentrations in the serum of the experimental mice were significantly increased compared with those of the control mice, which reflected the enzyme release from the infarcted myocardial cells. Histopathological examination showed different degrees of MI in the heart tissues of the experimental mice. The results indicate that an MI model in mice may be successfully established using CAL under the surgical conditions utilized in the present study. These conditions were cost effective and the results may be replicated by laboratories that are less well‑equipped.
View Figures

Figure 1

Figure 2

View References

1. 

Tarnavski O, McMullen JR, Schinke M, Nie Q, Kong S and Izumo S: Mouse cardiac surgery: comprehensive techniques for the generation of mouse models of human diseases and their application for genomic studies. Physiol Genomics. 16:349–360. 2004. View Article : Google Scholar : PubMed/NCBI

2. 

Cameron VA, Rademaker MT, Ellmers LJ, Espiner EA, Nicholls MG and Richards AM: Atrial (ANP) and brain natriuretic peptide (BNP) expression after myocardial infarction in sheep: ANP is synthesized by fibroblasts infiltrating the infarct. Endocrinology. 141:4690–4697. 2000.

3. 

Clark C, Foreman MI, Kane KA, McDonald FM and Parratt JR: Coronary artery ligation in anesthetized rats as a method for the production of experimental dysrhythmias and for the determination of infarct size. J Pharmacol Methods. 3:357–368. 1980. View Article : Google Scholar : PubMed/NCBI

4. 

Deten A, Hölzl A, Leicht M, Barth W and Zimmer HG: Changes in extracellular matrix and in transforming growth factor beta isoforms after coronary artery ligation in rats. J Mol Cell Cardiol. 33:1191–1207. 2001. View Article : Google Scholar : PubMed/NCBI

5. 

Mahaffey KW, Raya TE, Pennock GD, Morkin E and Goldman S: Left ventricular performance and remodeling in rabbits after myocardial infarction: effects of a thyroid hormone analogue. Circulation. 91:794–801. 1995. View Article : Google Scholar

6. 

Takano H, Qin Y, Hasegawa H, et al: Effects of G-CSF on left ventricular remodeling and heart failure after acute myocardial infarction. J Mol Med (Berl). 84:185–193. 2006.PubMed/NCBI

7. 

Zhou S, Chen LS, Miyauchi Y, et al: Mechanisms of cardiac nerve sprouting after myocardial infarction in dogs. Circ Res. 95:76–83. 2004. View Article : Google Scholar : PubMed/NCBI

8. 

Kumar D, Hacker TA, Buck J, et al: Distinct mouse coronary anatomy and myocardial infarction consequent to ligation. Coron Artery Dis. 16:41–44. 2005. View Article : Google Scholar : PubMed/NCBI

9. 

McGaffin KR, Zou B, McTiernan CF and O’Donnell CP: Leptin attenuates cardiac apoptosis after chronic ischaemic injury. Cardiovasc Res. 83:313–324. 2009. View Article : Google Scholar : PubMed/NCBI

10. 

Huang Y, Ma Y, Yang Y, et al: Effect of nuclear factor-κB inhibitor pyrrolidine dithiocarbamate on left ventricular remodeling after acute myocardial infarction in old mice. Chinese Circulation Journal. 26:378–381. 2011.(In Chinese).

11. 

Liu B, Du X and Zhou Y: Establishment of myocardial infarction mode in young mice of low body weight. Zhonghua Shi Yan Wai Ke Za Zhi. 29:132–134. 2012.(In Chinese).

12. 

Nie L, Li QG, Fan FD and Wang DJ: Establishment of mouse cardiac patch model. Zhonghua Shi Yan Wai Ke Za Zhi. 27:1703–1704. 2010.(In Chinese).

13. 

Yun W, Yu Y, Lu X, et al: Discussion of establishing mice model with myocardial infarction successfully. Journal Of China Medical University. 36:631–633. 2007.(In Chinese).

14. 

Zhao X, Zhou J, Ma R, et al: Study of establishing myocardial infarction model of mice. Sichuan Dong Wu. 25:627–629. 2006.(In Chinese).

15. 

Seta Y, Kanda T, Tanaka T, et al: Interleukin 18 in acute myocardial infarction. Heart. 84:668–669. 2000. View Article : Google Scholar : PubMed/NCBI

16. 

Suchalatha S and Shyamala Devi C: Effect of arogh - A polyherbal formulation on the marker enzymes in isoproterenol induced myocardial injury. Indian J Clin Biochem. 19:184–189. 2004. View Article : Google Scholar : PubMed/NCBI

17. 

van Laake LW, Passier R, Monshouwer-Kloots J, et al: Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction. Nat Protoc. 2:2551–2567. 2007.PubMed/NCBI

18. 

Wang H, Zhou J, Liu Z and Wang C: Injectable cardiac tissue engineering for the treatment of myocardial infarction. J Cell Mol Med. 14:1044–1055. 2010.PubMed/NCBI

19. 

Kolk MVV, Meyberg D, Deuse T, et al: LAD-ligation: a murine model of myocardial infarction. J Vis Exp. 14382009.PubMed/NCBI

20. 

Romson JL, Haack DW and Lucchesi BR: Electrical induction of coronary artery thrombosis in the ambulatory canine: a model for in vivo evaluation of anti-thrombotic agents. Thromb Res. 17:841–853. 1980. View Article : Google Scholar : PubMed/NCBI

21. 

McGaffin KR, Sun CK, Rager JJ, et al: Leptin signalling reduces the severity of cardiac dysfunction and remodelling after chronic ischaemic injury. Cardiovasc Res. 77:54–63. 2008. View Article : Google Scholar : PubMed/NCBI

22. 

Wang J, Bo H, Meng X, Wu Y, Bao Y and Li Y: A simple and fast experimental model of myocardial infarction in the mouse. Tex Heart Inst J. 33:290–293. 2006.PubMed/NCBI

23. 

Feng Q, Lu X, Jones DL, Shen J and Arnold JM: Increased inducible nitric oxide synthase expression contributes to myocardial dysfunction and higher mortality after myocardial infarction in mice. Circulation. 104:700–704. 2001.

24. 

Creemers EE, Davis JN, Parkhurst AM, et al: Deficiency of TIMP-1 exacerbates LV remodeling after myocardial infarction in mice. Am J Physiol Heart Circ Physiol. 284:H364–H371. 2003. View Article : Google Scholar : PubMed/NCBI

25. 

Orlic D, Kajstura J, Chimenti S, Bodine DM, Leri A and Anversa P: Bone marrow stem cells regenerate infarcted myocardium. Pediatr Transplant. 7(Suppl 3): S86–S88. 2003. View Article : Google Scholar

26. 

Janssens S, Pokreisz P, Schoonjans L, et al: Cardiomyocyte-specific overexpression of nitric oxide synthase 3 improves left ventricular performance and reduces compensatory hypertrophy after myocardial infarction. Circ Res. 94:1256–1262. 2004. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yue X, Yu H, Lin X, Liu K, Wang X, Zhou F, Zhao J and Zou B: Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice. Exp Ther Med 6: 341-346, 2013.
APA
Yue, X., Yu, H., Lin, X., Liu, K., Wang, X., Zhou, F. ... Zou, B. (2013). Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice. Experimental and Therapeutic Medicine, 6, 341-346. https://doi.org/10.3892/etm.2013.1154
MLA
Yue, X., Yu, H., Lin, X., Liu, K., Wang, X., Zhou, F., Zhao, J., Zou, B."Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice". Experimental and Therapeutic Medicine 6.2 (2013): 341-346.
Chicago
Yue, X., Yu, H., Lin, X., Liu, K., Wang, X., Zhou, F., Zhao, J., Zou, B."Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice". Experimental and Therapeutic Medicine 6, no. 2 (2013): 341-346. https://doi.org/10.3892/etm.2013.1154
Copy and paste a formatted citation
x
Spandidos Publications style
Yue X, Yu H, Lin X, Liu K, Wang X, Zhou F, Zhao J and Zou B: Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice. Exp Ther Med 6: 341-346, 2013.
APA
Yue, X., Yu, H., Lin, X., Liu, K., Wang, X., Zhou, F. ... Zou, B. (2013). Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice. Experimental and Therapeutic Medicine, 6, 341-346. https://doi.org/10.3892/etm.2013.1154
MLA
Yue, X., Yu, H., Lin, X., Liu, K., Wang, X., Zhou, F., Zhao, J., Zou, B."Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice". Experimental and Therapeutic Medicine 6.2 (2013): 341-346.
Chicago
Yue, X., Yu, H., Lin, X., Liu, K., Wang, X., Zhou, F., Zhao, J., Zou, B."Investigation into the optimal surgical conditions for coronary artery ligation for establishing a myocardial infarction model in mice". Experimental and Therapeutic Medicine 6, no. 2 (2013): 341-346. https://doi.org/10.3892/etm.2013.1154
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team