Induction of apoptosis in infantile hemangioma endothelial cells by propranolol

Tu,Jun-Bo; Ma,Rui-Zhao; Dong,Qiang; Jiang,Fei; Hu,Xiao-Yi; Li,Quan-Yan; Pattar,Parukjan; Zhang,Hao

doi:10.3892/etm.2013.1159

August 2013 Volume 6 Issue 2

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August 2013 Volume 6 Issue 2

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Article

Induction of apoptosis in infantile hemangioma endothelial cells by propranolol

Authors:
- Jun-Bo Tu
- Rui-Zhao Ma
- Qiang Dong
- Fei Jiang
- Xiao-Yi Hu
- Quan-Yan Li
- Parukjan Pattar
- Hao Zhang
View Affiliations / Copyright

Affiliations: Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Oral and Maxillofacial Surgery, Weihai Stomatological Hospital, Weihai, Shandong 264200, P.R. China
Pages: 574-578
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Published online on: June 14, 2013

https://doi.org/10.3892/etm.2013.1159
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Abstract

Propranolol, a non-selective β-blocker, is emerging as an effective treatment for complicated hemangiomas. The aim of this study was to investigate the molecular mechanism(s) underlying the therapeutic effects of propranolol against hemangiomas, using primary infantile hemangioma endothelial cells (IHECs). IHECs were treated with various concentrations of propranolol and morphological changes and apoptosis were assessed. Changes in the expression levels of apoptosis-related genes were examined. Annexin-V staining revealed that propranolol at 40, 50 and 60 µg/ml caused a concentration‑dependent increase in the apoptosis of IHECs. Morphological analyses revealed that exposure to 50 µg/ml propranolol resulted in typical apoptotic changes, including shrinkage, the formation of apoptotic bodies and retention of plasma membrane integrity. Gene expression analyses revealed that propranolol treatment led to a marked increase in the expression of caspase-8, cytochrome c, apoptosis-inducing factor, caspase-3 and poly (ADP-ribose) polymerase 1, as well as a concomitant reduction in lamin B1 expression. Our data collectively demonstrate that propranolol induces apoptosis of IHECs through activation of the intrinsic and extrinsic apoptotic pathways, which represents an important mechanism for its therapeutic effects against infantile hemangiomas.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Induction of apoptosis in infantile hemangioma endothelial cells by propranolol

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