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Print ISSN: 1792-0981 Online ISSN: 1792-1015
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August 2013 Volume 6 Issue 2

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model

  • Authors:
    • Baoli Li
    • Yongli Zhang
    • Bingyin Shi
    • Yahui Chen
    • Zhengxiang Zhang
    • Tao Liu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Medical College, Yan'an University, Shaanxi 716000, P.R. China, Department of General Medicine, Cardiovascular Hospital of Yan'an University, Shaanxi 716000, P.R. China, Department of Endocrinology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P.R. China, Department of Physiology, Medical College, Yan'an University, Xi'an, Shaanxi 716000, P.R. China
  • Pages: 562-566
    |
    Published online on: June 19, 2013
       https://doi.org/10.3892/etm.2013.1168
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Abstract

This study aimed to determine the effects and mechanisms of gardenia oil on bone density and bone biomechanics in ovariectomized female rats. An ovariectomized rat model was established and the rats were administered various doses of gardenia oil. Rats administered diethylstilbestrol or saline served as the positive and the untreated controls, respectively. All rats received the same surgery, with the exception of the ovariectomy in the sham group. The levels of serum 17β-estradiol, follicle-stimulating hormone, luteinizing hormone, alkaline phosphatase (ALP) and calcium, and the bone material density (BMD), maximum stress and maximum strain were determined. The expression of COX-2 was also determined by immunoblotting and quantitative PCR (qPCR). Gardenia oil increased the serum levels of 17β-estradiol, the BMD, and the maximum stress and maximum strain of bones. The levels of COX-2 protein and COX-2 mRNA were significantly increased in the gardenia oil‑treated rats. In conclusion, gardenia oil increases estradiol levels and BMD in an ovariectomized rat model. The effects of gardenia oil are associated with upregulation of the expression of COX-2.
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Copy and paste a formatted citation
Spandidos Publications style
Li B, Zhang Y, Shi B, Chen Y, Zhang Z and Liu T: Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model. Exp Ther Med 6: 562-566, 2013.
APA
Li, B., Zhang, Y., Shi, B., Chen, Y., Zhang, Z., & Liu, T. (2013). Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model. Experimental and Therapeutic Medicine, 6, 562-566. https://doi.org/10.3892/etm.2013.1168
MLA
Li, B., Zhang, Y., Shi, B., Chen, Y., Zhang, Z., Liu, T."Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model". Experimental and Therapeutic Medicine 6.2 (2013): 562-566.
Chicago
Li, B., Zhang, Y., Shi, B., Chen, Y., Zhang, Z., Liu, T."Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model". Experimental and Therapeutic Medicine 6, no. 2 (2013): 562-566. https://doi.org/10.3892/etm.2013.1168
Copy and paste a formatted citation
x
Spandidos Publications style
Li B, Zhang Y, Shi B, Chen Y, Zhang Z and Liu T: Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model. Exp Ther Med 6: 562-566, 2013.
APA
Li, B., Zhang, Y., Shi, B., Chen, Y., Zhang, Z., & Liu, T. (2013). Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model. Experimental and Therapeutic Medicine, 6, 562-566. https://doi.org/10.3892/etm.2013.1168
MLA
Li, B., Zhang, Y., Shi, B., Chen, Y., Zhang, Z., Liu, T."Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model". Experimental and Therapeutic Medicine 6.2 (2013): 562-566.
Chicago
Li, B., Zhang, Y., Shi, B., Chen, Y., Zhang, Z., Liu, T."Gardenia oil increases estradiol levels and bone material density by a mechanism associated with upregulation of COX-2 expression in an ovariectomized rat model". Experimental and Therapeutic Medicine 6, no. 2 (2013): 562-566. https://doi.org/10.3892/etm.2013.1168
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