Combined treatment with FK506 and nerve growth factor for spinal cord injury in rats

Chen,Guang; Zhang,Zhen; Wang,Shouyu; Lv,Decheng

doi:10.3892/etm.2013.1254

Combined treatment with FK506 and nerve growth factor for spinal cord injury in rats

Authors:
- Guang Chen
- Zhen Zhang
- Shouyu Wang
- Decheng Lv
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Affiliations: Department of Orthopedic Surgery, First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011, P.R. China
Published online on: August 7, 2013 https://doi.org/10.3892/etm.2013.1254
Pages: 868-872

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Abstract

Following spinal cord injury in rats, FK506 is able to protect local nerve tissue, promote neural regeneration, reduce neuronal apoptosis and accelerate the recovery of spinal cord functions. Nerve growth factor (NGF) is important in the regulation of central and peripheral nerve cell regeneration, growth differentiation and functions. Previous studies have shown that FK506 and NGF exhibit a synergistic effect in the treatment of peripheral nerve injury; however, it remains unclear whether the synergistic effect is present in the treatment of spinal cord injury. In this study, we combined FK506 and NGF for the treatment of spinal cord injury in rats. The NF200 protein expression in rats with spinal cord injury was determined using immunohistochemical staining and NF200 mRNA expression levels were observed using the reverse transcription‑polymerase chain reaction method. The restoration of spinal cord functions was evaluated using the Basso, Beattie and Bresnahan score. The results demonstrated that the combined treatment significantly enhanced the expression of NF200 and improved spinal cord functions compared with the results of the single treatment. Our experimental observations indicated that FK506 and NGF exhibit a synergistic effect in the treatment of spinal cord injury in rats and that the combined treatment may effectively promote neural regeneration and functional recovery in rats following spinal cord injury.

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