Sulfated modification and anti‑tumor activity of laminarin

Ji,Chen-Feng; Ji,Yu-Bin; Meng,De-You

doi:10.3892/etm.2013.1277

November 2013 Volume 6 Issue 5

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November 2013 Volume 6 Issue 5

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Article

Sulfated modification and anti‑tumor activity of laminarin

Authors:
- Chen-Feng Ji
- Yu-Bin Ji
- De-You Meng
View Affiliations / Copyright

Affiliations: Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin University of Commerce, Harbin, Heilongjiang 150076, P.R. China
Pages: 1259-1264
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Published online on: August 29, 2013

https://doi.org/10.3892/etm.2013.1277
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Abstract

The aim of this study was to investigate the sulfated modification of laminarin and the changes in structure and antitumor activity. The chlorosulfonic acid‑pyridine method was applied for sulfated modification. The molecular weights of laminarin and laminarin sulfate (LAMS) were measured by high-performance liquid chromatography (HPLC), and IR and NMR spectra were also recorded. The surface conformations of laminarin and LAMS were observed with a scanning electron microscope. The antitumor activities of the two polysaccharides were also evaluated using an MTT assay. LAMS with a sulfate content of 45.92% and a molecular weight of 16,000 was synthesized. The IR spectra of laminarin and LAMS showed the characteristic absorption peaks of a polysaccharide, and LAMS also had the characteristic absorption peaks of sulfate moieties. The NMR spectra showed that laminarin and LAMS had β-(1→3) glycosidic bonds forming the main chain, and sulfate substitution was at the hydroxyl groups of C2 and C6. Under the scanning electron microscope, there were clear differences in surface conformation between laminarin and LAMS; laminarin was cloud-like and spongy, while LAMS was block‑like and flaky. The MTT results showed that laminarin and LAMS had inhibitory effects on LoVo cell growth, and the antitumor activity of LAMS was higher than that of laminarin at the same concentration. This suggests that sulfated modification was able to change the laminarin structure and markedly enhance the antitumor activity.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Sulfated modification and anti‑tumor activity of laminarin

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