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Article

Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion

  • Authors:
    • Jinmei Shen
    • Gan Fu
    • Lili Jiang
    • Junmei Xu
    • Li Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 1359-1364
    |
    Published online on: September 26, 2013
       https://doi.org/10.3892/etm.2013.1317
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Abstract

Reperfusion injury is tissue damage caused by the re‑supply of blood following a period of ischemia in tissues. Intestinal ischemia‑reperfusion injury (IRI) is an extremely common clinical event associated with distant organ injury. The intestine serves as the initial organ of multi‑system organ dysfunction syndrome. It is extremely important to identify a method to protect against IRI, as it is a key factor associated with morbidity and mortality in patients. In the present study, the protective effects of pretreatment with dexmedetomidine hydrochloride were investigated. Rats were divided into six groups and models of intestinal ischemia were created in the five groups. Certain groups were pretreated with dexmedetomidine hydrochloride. The levels of TNF‑α and IL‑6 were measured by enzyme‑linked immunosorbent assay in order to evaluate the injury. Tissue sections were stained with hematoxylin and eosin to visualize the damage. qPCR and western blotting were performed to examine the inflammatory status. Pretreatment with various doses of dexmedetomidine hydrochloride significantly reduced the pathological scores and the inflammatory reaction. The levels of TNF‑α, IL‑6, TLR4 and MyD88 were decreased in the dexmedetomidine hydrochloride treatment groups compared with those in the sham control and untreated ischemia reperfusion groups. The results of the present study indicate that pretreatment with dexmedetomidine hydrochloride may be a useful method of reducing the damage caused by IRI.
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Copy and paste a formatted citation
Spandidos Publications style
Shen J, Fu G, Jiang L, Xu J and Li L: Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion. Exp Ther Med 6: 1359-1364, 2013.
APA
Shen, J., Fu, G., Jiang, L., Xu, J., & Li, L. (2013). Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion. Experimental and Therapeutic Medicine, 6, 1359-1364. https://doi.org/10.3892/etm.2013.1317
MLA
Shen, J., Fu, G., Jiang, L., Xu, J., Li, L."Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion". Experimental and Therapeutic Medicine 6.6 (2013): 1359-1364.
Chicago
Shen, J., Fu, G., Jiang, L., Xu, J., Li, L."Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion". Experimental and Therapeutic Medicine 6, no. 6 (2013): 1359-1364. https://doi.org/10.3892/etm.2013.1317
Copy and paste a formatted citation
x
Spandidos Publications style
Shen J, Fu G, Jiang L, Xu J and Li L: Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion. Exp Ther Med 6: 1359-1364, 2013.
APA
Shen, J., Fu, G., Jiang, L., Xu, J., & Li, L. (2013). Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion. Experimental and Therapeutic Medicine, 6, 1359-1364. https://doi.org/10.3892/etm.2013.1317
MLA
Shen, J., Fu, G., Jiang, L., Xu, J., Li, L."Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion". Experimental and Therapeutic Medicine 6.6 (2013): 1359-1364.
Chicago
Shen, J., Fu, G., Jiang, L., Xu, J., Li, L."Effect of dexmedetomidine pretreatment on lung injury following intestinal ischemia‑reperfusion". Experimental and Therapeutic Medicine 6, no. 6 (2013): 1359-1364. https://doi.org/10.3892/etm.2013.1317
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