Platelet‑rich plasma, plasma rich in growth factors and simvastatin in the regeneration and repair of alveolar bone

Rivera,César; Monsalve,Francisco; Salas,Juan; Morán,Andrea; Suazo,Iván

doi:10.3892/etm.2013.1327

Platelet‑rich plasma, plasma rich in growth factors and simvastatin in the regeneration and repair of alveolar bone

Authors:
- César Rivera
- Francisco Monsalve
- Juan Salas
- Andrea Morán
- Iván Suazo
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Affiliations: Unit of Histology and Embryology, Faculty of Health Sciences, University of Talca, Talca 3460000, Chile, Unit of Physiology and Pharmacology, Department of Basic Biomedical Sciences, Faculty of Health Sciences, University of Talca, Talca 3460000, Chile, School of Dentistry, Faculty of Health Sciences, University of Talca, Talca 3460000, Chile, Autonomous University of Chile, Santiago 7500000, Chile
Published online on: October 7, 2013 https://doi.org/10.3892/etm.2013.1327
Pages: 1543-1549

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Abstract

Platelet preparations promote bone regeneration by inducing cell migration, proliferation and differentiation in the area of the injury, which are essential processes for regeneration. In addition, several studies have indicated that simvastatin (SIMV), widely used for the treatment of hypercholesterolemia, stimulates osteogenesis. The objective of this study was to evaluate the effects of treatment with either platelet‑rich plasma (PRP) or plasma rich in growth factors (PRGF) in combination with SIMV in the regeneration and repair of alveolar bone. The jaws of Sprague Dawley rats (n=18) were subjected to rotary instrument‑induced bone damage (BD). Animals were divided into six groups: BD/H2O (n=3), distilled water without the drug and alveolar bone damage; BD/H2O/PRP (n=3), BD and PRP; BD/H2O/PRGF (n=3), BD and PRGF; BD/SIMV (n=3), BD and water with SIMV; BD/SIMV/PRP (n=3), BD, PRP and SIMV; and BD/SIMV/PRGF (n=3), BD, PRGF and SIMV. Conventional histological analysis (hematoxylin and eosin staining) revealed that the BD/SIMV group showed indicators for mature bone tissue, while the BD/SIMV/PRP and BD/SIMV/PRGF groups showed the coexistence of indicators for mature and immature bone tissue, with no statistical differences between the platelet preparations. Simvastatin did not improve the effect of platelet‑rich plasma and plasma rich in growth factors. It was not possible to determine which platelet preparation produced superior effects.

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