Effect of tanshinone IIA on cardiomyocyte hypertrophy and apoptosis in spontaneously hypertensive rats

Jiang,F. L.; Leo,S.; Wang,X. G.; Li,H.; Gong,L. Y.; Kuang,Y.; Xu,X. F.

doi:10.3892/etm.2013.1339

Effect of tanshinone IIA on cardiomyocyte hypertrophy and apoptosis in spontaneously hypertensive rats

Authors:
- F. L. Jiang
- S. Leo
- X. G. Wang
- H. Li
- L. Y. Gong
- Y. Kuang
- X. F. Xu
View Affiliations

Affiliations: Department of Cardiology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
Published online on: October 10, 2013 https://doi.org/10.3892/etm.2013.1339
Pages: 1517-1521

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

In the present study, the effects of tanshinone IIA (TSN) on the prevention of left ventricular hypertrophy (LVH) and apoptotic processes were observed in spontaneously hypertensive rats (SHRs). A total of 18 SHRs (age, 8 weeks) were randomly divided into three groups. The SHRs in the control group (group S8) were sacrificed at week 8 of the experiment. The SHRs in the treatment group (group D18) and the placebo group (group S18) were injected with TSN and distilled water (1 ml/kg body weight/day), respectively, for 10 weeks, commencing at week 8, and were subsequently sacrificed at week 18. The systolic blood pressure (SBP) and left ventricular mass index (LVMI) were determined. Using hematoxylin and eosin and van Gieson staining, together with immunohistological methods, cardiomyocyte size and diameter, collagen volume fraction (CVF) and perivascular circumferential area (PVCA) were measured. Evaluation of Bcl‑2, Bax and p53 expression levels for apoptosis analysis was performed using western blotting. It was observed that the SBP, LVMI, cardiomyocyte size and diameter, CVF, PCVA and cardiomyocyte apoptosis index (Bax and p53 expression) were increased significantly in group S18 compared with group S8. However, Bcl‑2 expression levels were decreased in group S18 compared with group S8. The administration of TSN in group D18 resulted in higher Bcl‑2 expression levels and significantly decreased LVMI, cardiomyocyte size and diameter, CVF, PCVA, Bax and p53 expression levels compared with group S18. LVH and apoptosis of the cardiac tissues increased with the increasing age of the SHRs. TSN may inhibit the development of LVH and decrease the level of apoptosis in SHRs, possibly via the upregulation of Bcl‑2 and the downregulation of Bax and p53 expression.

View Figures

View References

1	Fox E, Taylor H, Andrew M, et al: Body mass index and blood pressure influences on left ventricular mass and geometry in African Americans: The Atherosclerotic Risk In Communities (ARIC) Study. Hypertension. 44:55–60. 2004. View Article : Google Scholar : PubMed/NCBI
2	Sundström J, Lind L, Arnlöv J, Zethelius B, Andrén B and Lithell HO: Echocardiographic and electrocardiographic diagnoses of left ventricular hypertrophy predict mortality independently of each other in a population of elderly men. Circulation. 103:2346–2351. 2001.PubMed/NCBI
3	Levy D, Garrison RJ, Savage DD, Kannel WB and Castelli WP: Prognostic implications of echocardiographically determined left ventricular mass in the Framingham Heart Study. N Engl J Med. 322:1561–1566. 1990. View Article : Google Scholar : PubMed/NCBI
4	Chan P, Liu IM, Li YX, Yu WJ and Cheng JT: Antihypertension induced by tanshinone IIA isolated from the roots of Salvia miltiorrhiza. Evid Based Complement Alternat Med. 2011:3926272011. View Article : Google Scholar : PubMed/NCBI
5	Tang FT, Cao Y, Wang TQ, et al: Tanshinone IIA attenuates atherosclerosis in ApoE(−/−) mice through down-regulation of scavenger receptor expression. Eur J Pharmacol. 650:275–284. 2011.
6	Shan H, Li X, Pan Z, et al: Tanshinone IIA protects against sudden cardiac death induced by lethal arrhythmias via repression of microRNA-1. Br J Pharmacol. 158:1227–1235. 2009. View Article : Google Scholar : PubMed/NCBI
7	Jang SI, Kim HJ, Kim YJ, Jeong SI and You YO: Tanshinone IIA inhibits LPS-induced NF-kappaB activation in RAW 264.7 cells: possible involvement of the NIK-IKK, ERK1/2, p38 and JNK pathways. Eur J Pharmacol. 542:1–7. 2006. View Article : Google Scholar : PubMed/NCBI
8	Gong LY, Zheng Z and Xiong W: The effect of Salvia miltiorrhiza Bge on preventing left ventricular hypertrophy in spontaneously hypertensive rats. Chinese Journal of Hypertension. 11:257–259. 2003.(In Chinese).
9	Yang L, Zou X, Liang Q, et al: Sodium tanshinone IIA sulfonate depresses angiotensin II-induced cardiomyocyte hypertrophy through MEK/ERK pathway. Exp Mol Med. 39:65–73. 2007. View Article : Google Scholar : PubMed/NCBI
10	Velez Rueda JO, Palomeque J and Mattiazzi A: Early apoptosis in different models of cardiac hypertrophy induced by high renin-angiotensin system activity involves CaMKII. J Appl Physiol. 112:2110–2120. 2012.PubMed/NCBI
11	Messerli FH: Hypertension and sudden cardiac death. Am J Hypertens. 12:181S–188S. 1999. View Article : Google Scholar
12	Kukhta VK, Marozkina NV, Sokolchik IG and Bogaturova EV: Molecular mechanisms of apoptosis. Ukr Biokhim Zh. 75:5–9. 2003.
13	Dlamini Z, Mbita Z and Zungu M: Genealogy, expression, and molecular mechanisms in apoptosis. Pharmacol Ther. 101:1–15. 2004. View Article : Google Scholar
14	Goga LM, Vasiliu OM and Ionescu CR: Molecular mechanisms of apoptosis. Fas and TNF systems. ICE protease system. Bcl-2 family. Rev Med Chir Soc Med Nat Iasi. 105:23–29. 2001.(In Romanian).
15	Zheng Z, Han HJ and Ren DH: Effects of Salvia miltiorrhiza Bge on left ventricular hypertrophy and cardiac aldosterone in spontaneously hypertensive rats. Chin J Emerg Med. 11:22–24. 2002.(In Chinese).
16	Liu F and Zheng Z: Influence of Radix Salviac miltiorrhizae(RSM) on cardiac myocyte levels of transforming growth factor-β1 and preventive effects of RSM on left ventricular hypertrophy in spontaneously hypertensive rats. Chin J Crit Care Med. 23:71–73. 2003.(In Chinese).
17	Sun LP and Zheng Z: The effect of Salvia miltiorrhiza Bge on left ventricular hypertrophy and the expression of tumor necrosis factor-alpha in spontaneously hypertensive rats. Chin J Hypertens. 12:238–241. 2004.(In Chinese).
18	Jiang FL, Feng J and Zheng Z: Effects of tanshinone II on the mRNA expression of c-fos, c-myc and c-jun in angiotensin II-induced hypertrophy of cardiomyocytes. Chin Pharmacol Bull. 23:55–59. 2007.(In Chinese).