Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells

Liu,Wei-Wu; Liu,Yang; Liang,Shuo; Wu,Jia-Hui; Wang,Zhi-Cheng; Gong,Shou-Liang

doi:10.3892/etm.2013.1351

Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells

Authors:
- Wei-Wu Liu
- Yang Liu
- Shuo Liang
- Jia-Hui Wu
- Zhi-Cheng Wang
- Shou-Liang Gong
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Affiliations: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: October 16, 2013 https://doi.org/10.3892/etm.2013.1351
Pages: 1560-1564

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Abstract

A conditionally replicative adenoviral (CRAd) vector, designated as CRAd.pEgr-1-Smac, that promotes the overexpression of second mitochondria-derived activator of caspase (Smac) when stimulated by hypoxia and radiation was constructed. MDA‑MB‑231 cells were transfected with CRAd.pEgr-1-Smac and treated with 4-Gy X-rays. The hypoxic status in cancer cells was mimicked with the chemical reagent CoCl2. Smac protein expression was measured by a western blotting assay and cell proliferation was detected with the MTT assay. The cell cycle progression and apoptotic percentage were measured by flow cytometry with PI and Annexin V-FITC staining kits, respectively, following the irradiation of the transfected cells with 4-Gy X-rays. The results showed that CRAd.pEgr-1-Smac was able to increase the Smac protein expression induced by hypoxia and radiation, inhibit cell proliferation and promote apoptosis. Therefore, this method of gene-radiotherapy is indicated to be an ideal strategy for the treatment of breast cancer.

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