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Article

Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells

  • Authors:
    • Wei-Wu Liu
    • Yang Liu
    • Shuo Liang
    • Jia-Hui Wu
    • Zhi-Cheng Wang
    • Shou-Liang Gong
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 1560-1564
    |
    Published online on: October 16, 2013
       https://doi.org/10.3892/etm.2013.1351
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Abstract

A conditionally replicative adenoviral (CRAd) vector, designated as CRAd.pEgr-1-Smac, that promotes the overexpression of second mitochondria-derived activator of caspase (Smac) when stimulated by hypoxia and radiation was constructed. MDA‑MB‑231 cells were transfected with CRAd.pEgr-1-Smac and treated with 4-Gy X-rays. The hypoxic status in cancer cells was mimicked with the chemical reagent CoCl2. Smac protein expression was measured by a western blotting assay and cell proliferation was detected with the MTT assay. The cell cycle progression and apoptotic percentage were measured by flow cytometry with PI and Annexin V-FITC staining kits, respectively, following the irradiation of the transfected cells with 4-Gy X-rays. The results showed that CRAd.pEgr-1-Smac was able to increase the Smac protein expression induced by hypoxia and radiation, inhibit cell proliferation and promote apoptosis. Therefore, this method of gene-radiotherapy is indicated to be an ideal strategy for the treatment of breast cancer.
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1 

DeSantis C, Siegel R, Bandi P and Jemal A: Breast cancer statistics, 2011. CA Cancer J Clin. 61:409–418. 2011. View Article : Google Scholar

2 

Kwong DL, Sham JS, Leung LH, Cheng AC, Ng WM, Kwong PW, Lui WM, Yau CC, Wu PM, Wei W and Au G: Preliminary results of radiation dose escalation for locally advanced nasopharyngeal carcinoma. Int J Radiat Oncol Biol Phys. 64:374–381. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Zheng AQ, Song XR, Yu JM, Wei L and Wang XW: Liposome transfected to plasmid-encoding endostatin gene combined with radiotherapy inhibits liver cancer growth in nude mice. World J Gastroenterol. 11:4439–4442. 2005.PubMed/NCBI

4 

Harari PM and Huang SM: Head and neck cancer as a clinical model for molecular targeting of therapy: combining EGFR blockade with radiation. Int J Radiat Oncol Biol Phys. 49:427–433. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Liu LL, Smith MJ, Sun BS, Wang GJ, Redmond HP and Wang JH: Combined IFN-gamma-endostatin gene therapy and radiotherapy attenuates primary breast tumor growth and lung metastases via enhanced CTL and NK cell activation and attenuated tumor angiogenesis in a murine model. Ann Surg Oncol. 16:1403–1411. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Yang W and Li XY: Anti-tumor effect of pEgr-interferon-γ-endostatin gene-radiotherapy in mice bearing Lewis lung carcinoma and its mechanism. Chin Med J (Engl). 118:296–301. 2005.PubMed/NCBI

7 

Li Y, Guo C, Wang Z, Gong P, Sun Z and Gong S: Enhanced effects of TRAIL-endostatin-based double-gene-radiotherapy on suppressing growth, promoting apoptosis and inducing cell cycle arrest in vascular endothelial cells. J Huazhong Univ Sci Technolog Med Sci. 32:167–172. 2012. View Article : Google Scholar

8 

Wu C, Li X and Tian M: Effect of pEgr-TNFalpha gene radiotherapy on mice melanoma. Melanoma Res. 15:185–190. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Zhang Y, Yu XH, Zha X and Kong W: Adenovirus-mediated HSV-TK gene therapy using hTERT promoter in CNE cells in vitro. Chem Res Chinese Universities. 25:60–63. 2009.

10 

Wang WD, Chen ZT, Li DZ, Duan YZ, Wang ZX and Cao ZH: HSV-TK gene therapy of lung adenocarcinoma xenografts using a hypoxia/radiation dual-sensitive promoter. Ai Zheng. 23:788–793. 2004.(In Chinese).

11 

Mizutani Y, Nakanishi H, Yamamoto K, Li YN, Matsubara H, Mikami K, Okihara K, Kawauchi A, Bonavida B and Miki T: Downregulation of Smac/DIABLO expression in renal cell carcinoma and its prognostic significance. J Clin Oncol. 23:448–454. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Pluta P, Cebula-Obrzut B, Ehemann V, Pluta A, Wierzbowska A, Piekarski J, Bilski A, Nejc D, Kordek R, Robak T, Smolewski P and Jeziorski A: Correlation of Smac/DIABLO protein expression with the clinico-pathological features of breast cancer patients. Neoplasma. 58:430–435. 2011. View Article : Google Scholar

13 

Fulda S, Wick W, Weller M and Debatin KM: Smac agonists sensitize for Apo2L/TRAIL or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo. Nat Med. 8:808–815. 2002.PubMed/NCBI

14 

Lok CN and Ponka P: Identification of a hypoxia response element in the transferin receptor gene. J Biol Chem. 274:24147–24152. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Okino ST, Chichester CH and Whitlock JP Jr: Hypoxia-inducible mammalian gene expression analyzed in vivo at a TATA-driven promoter and at an initiator-driven promoter. J Biol Chem. 273:23837–23843. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Kwon OJ, Kim PH, Huyn S, Wu L, Kim M and Yun CO: A hypoxia- and α-fetoprotein-dependent oncolytic adenovirus exhibits specific killing of hepatocellular carcinomas. Clin Cancer Res. 16:6071–6082. 2010.

17 

Barnes MN, Coolidge CJ, Hemminki A, Alvarez RD and Curiel DT: Conditionally replicative adenoviruses for ovarian cancer therapy. Mol Cancer Ther. 1:435–439. 2002.PubMed/NCBI

18 

Wu JH, Wang HF, Wang ZC, Xu K, Qi YL, Li JH, Gong SL and Liu Y and Liu Y: Conditionally replicating adenovirus combined with gene-targeted radiotherapy induces apoptosis via TRAIL death receptors in MDA-MB-231 cells. Mol Med Rep. 8:299–305. 2013.PubMed/NCBI

19 

Dai M, Cui P, Yu M, Han J, Li H and Xiu R: Melatonin modulates the expression of VEGF and HIF-1 alpha induced by CoCl2 in cultured cancer cells. J Pineal Res. 44:121–126. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Liu G, Gong P, Zhao H, Wang Z, Gong S and Cai L: Effect of low-level radiation on the death of male germ cells. Radiat Res. 165:379–389. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Yang SW, Chanda D, Coody JJ, Rivera AA, Waehler R, Siegal GP, Douglas JT and Ponnazhagan S: Conditionally replicating adenovirus expressing TIMP2 in creases survival in a mouse model of disseminated ovarian cancer. PLoS One. 6:e251312011. View Article : Google Scholar : PubMed/NCBI

22 

Zheng FQ, Xu Y, Yang RJ, Wu B, Tan XH, Qin YD and Zhang QW: Combination effect of oncolytic adenovirus therapy and herpes simplex virus thymidine kinase/ganciclovir in hepatic carcinoma animal models. Acta Pharmacol Sin. 30:617–627. 2009. View Article : Google Scholar

23 

Senzer N, Mani S, Rosemurgy A, Nemunaitis J, Cunningham C, Guha C, Bayol N, Gillen M, Chu K, Rasmussen C, Rasmussen H, Kufe D, Weichselbaum R and Hanna N: TNFerade biologic, an adenovector with a radiation-inducible promoter, carrying the human tumor necrosis factor alpha gene: a phase I study in patients with solid tumors. J Clin Oncol. 22:592–601. 2004. View Article : Google Scholar

24 

Kasuya H, Takeda S, Nomoto S and Nakao A: The potential of oncolytic virus therapy for pancreatic cancer. Cancer Gene Ther. 12:725–736. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Teyssier F, Bay JO, Dionet C and Verrelle P: Cell cycle regulation after exposure to ionizing radiation. Bull Cancer. 86:345–357. 1999.(In French).

26 

Deplanque G, Céraline J, Mah-Becherel MC, Cazenave JP, Bergerat JP and Klein-Soyer C: Caffeine and the G2/M block override: a concept resulting from a misleading cell kinetic delay, independent of functional p53. Int J Cancer. 94:363–369. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Guo C, Li Y, Zhang H, Wang Z, Jin M, Zhang L, An L, Hu G, Liu X, Liu Y, Du H and Sun Z: Enhancement of antiproliferative and proapoptotic effects of cadmium chloride combined with hSmac in hepatocellular carcinoma cells. Chemotherapy. 57:27–34. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu W, Liu Y, Liang S, Wu J, Wang Z and Gong S: Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells. Exp Ther Med 6: 1560-1564, 2013.
APA
Liu, W., Liu, Y., Liang, S., Wu, J., Wang, Z., & Gong, S. (2013). Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells. Experimental and Therapeutic Medicine, 6, 1560-1564. https://doi.org/10.3892/etm.2013.1351
MLA
Liu, W., Liu, Y., Liang, S., Wu, J., Wang, Z., Gong, S."Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells". Experimental and Therapeutic Medicine 6.6 (2013): 1560-1564.
Chicago
Liu, W., Liu, Y., Liang, S., Wu, J., Wang, Z., Gong, S."Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells". Experimental and Therapeutic Medicine 6, no. 6 (2013): 1560-1564. https://doi.org/10.3892/etm.2013.1351
Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Liu Y, Liang S, Wu J, Wang Z and Gong S: Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells. Exp Ther Med 6: 1560-1564, 2013.
APA
Liu, W., Liu, Y., Liang, S., Wu, J., Wang, Z., & Gong, S. (2013). Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells. Experimental and Therapeutic Medicine, 6, 1560-1564. https://doi.org/10.3892/etm.2013.1351
MLA
Liu, W., Liu, Y., Liang, S., Wu, J., Wang, Z., Gong, S."Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells". Experimental and Therapeutic Medicine 6.6 (2013): 1560-1564.
Chicago
Liu, W., Liu, Y., Liang, S., Wu, J., Wang, Z., Gong, S."Hypoxia- and radiation‑induced overexpression of Smac by an adenoviral vector and its effects on cell cycle and apoptosis in MDA‑MB‑231 human breast cancer cells". Experimental and Therapeutic Medicine 6, no. 6 (2013): 1560-1564. https://doi.org/10.3892/etm.2013.1351
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