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Article

Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins

  • Authors:
    • Xiaohuan Zhong
    • Huixin Wang
    • Xinchun Jian
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 295-299
    |
    Published online on: November 8, 2013
       https://doi.org/10.3892/etm.2013.1394
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Abstract

Insulin has been proposed to be a positive regulator of osteoblast proliferation and bone formation. In vivo mechanical loading is essential for maintaining skeletal integrity and bone mass. Since insulin and mechanical force activate similar signaling pathways in osteoblasts, it was hypothesized that insulin may affect mechanical stimulation in osteoblasts. The present study tested the hypothesis that insulin augments mechanical strain‑induced signaling and early gene expression in MG63 cells via activation of the extracellular signal‑regulated kinase (ERK) pathway and cyclooxygenase‑2 (Cox‑2) expression. Western blot analysis and quantitative polymerase chain reaction demonstrated respectively that insulin enhanced mechanical strain‑induced ERK phosphorylation and Cox‑2 expression levels in a dose‑dependent manner. The effect of insulin on mechanical strain‑induced Cox‑2 expression was inhibited by blockade of the ERK pathway. In addition, echistatin, an inhibitor of integrin function, prevented the effects of insulin on mechanical strain‑induced ERK phosphorylation and Cox‑2 expression. The data obtained from this study suggested that insulin augments mechanical strain‑induced Cox‑2 expression levels via integrin‑dependent activation of the ERK pathway in osteoblasts.
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Copy and paste a formatted citation
Spandidos Publications style
Zhong X, Wang H and Jian X: Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins. Exp Ther Med 7: 295-299, 2014.
APA
Zhong, X., Wang, H., & Jian, X. (2014). Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins. Experimental and Therapeutic Medicine, 7, 295-299. https://doi.org/10.3892/etm.2013.1394
MLA
Zhong, X., Wang, H., Jian, X."Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins". Experimental and Therapeutic Medicine 7.1 (2014): 295-299.
Chicago
Zhong, X., Wang, H., Jian, X."Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins". Experimental and Therapeutic Medicine 7, no. 1 (2014): 295-299. https://doi.org/10.3892/etm.2013.1394
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong X, Wang H and Jian X: Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins. Exp Ther Med 7: 295-299, 2014.
APA
Zhong, X., Wang, H., & Jian, X. (2014). Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins. Experimental and Therapeutic Medicine, 7, 295-299. https://doi.org/10.3892/etm.2013.1394
MLA
Zhong, X., Wang, H., Jian, X."Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins". Experimental and Therapeutic Medicine 7.1 (2014): 295-299.
Chicago
Zhong, X., Wang, H., Jian, X."Insulin augments mechanical strain‑induced ERK activation and cyclooxygenase‑2 expression in MG63 cells through integrins". Experimental and Therapeutic Medicine 7, no. 1 (2014): 295-299. https://doi.org/10.3892/etm.2013.1394
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