Effect of 15‑hydroxyprostaglandin dehydrogenase gene on the proliferation of gastric cancer cell murine forestomach carcinoma

Li,Lihua; Yang,Fu; Wang,Xiaojie; Hu,Jing; Yang,Libo; Tang,Chunming; Wu,Yunhua; Miao,Kun; Liu,Rui; Shou,Tao

doi:10.3892/etm.2013.1404

2014-January Volume 7 Issue 1

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2014-January Volume 7 Issue 1

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Article

Effect of 15‑hydroxyprostaglandin dehydrogenase gene on the proliferation of gastric cancer cell murine forestomach carcinoma

Authors:
- Lihua Li
- Fu Yang
- Xiaojie Wang
- Jing Hu
- Libo Yang
- Chunming Tang
- Yunhua Wu
- Kun Miao
- Rui Liu
- Tao Shou
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Affiliations: Department of Oncology, The First Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China
Pages: 290-294
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Published online on: November 12, 2013

https://doi.org/10.3892/etm.2013.1404
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Abstract

The aim of the present study was to construct the eukaryotic expression vector pcDNA3.1/15‑PGDH. The vector was used to transfect mouse murine forestomach carcinoma (MFC) cancer cells and observe the effects of 15‑hydroxyprostaglandin dehydrogenase (15‑PGDH) on the proliferation of MFC. pcDNA3.1/15‑PGDH was constructed using gene recombination technology and the vector was used to transfect MFC cells to build a stable transfected cell strain. The expression levels of 15‑PGDH in the transfected cells were detected using reverse transcription polymerase chain reaction. Optical Density (OD) values were determined using an MTT assay and used to draw cell growth curves. The effects of 15‑PGDH on the proliferation of MFC were observed using a clone formation experiment. Following successful transfection by 15‑PGDH, the relative expression levels of 15‑PGDH in the MFC/15‑PGDH cells were significantly higher (1.06±0.08) (P<0.01) compared with the empty plasmid‑transfected group (0.22±0.01) and the untransfected group (0.21±0.01). Following transfection by 15‑PGDH, cell growth was markedly inhibited. The MTT results showed that on days 4, 6 and 8, the 15‑PGDH‑transfected group had a low OD on average, which was significantly different (P<0.05) from the empty plasmid‑transfected group or the untransfected group. The 15‑PGDH‑transfected group had a plating efficiency of 18%, and compared with the untransfected group (63%) and the empty plasmid‑transfected group (59%), clone formation was significantly inhibited (P<0.01). Results of the present study indicate that transfection by 15‑PGDH may significantly inhibit the proliferation and clone formation of MFC cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of 15‑hydroxyprostaglandin dehydrogenase gene on the proliferation of gastric cancer cell murine forestomach carcinoma

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