Expression of N‑cadherin proteins in myocardial hypertrophy in rats

Mu,Lingmin; Jing,Changqin; Guo,Zhikun

doi:10.3892/etm.2013.1431

Expression of N‑cadherin proteins in myocardial hypertrophy in rats

Authors:
- Lingmin Mu
- Changqin Jing
- Zhikun Guo
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Affiliations: Morphological Laboratory, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Life Science and Technology Department, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Key Open Laboratory for Tissue Regeneration in Henan Province, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Published online on: November 28, 2013 https://doi.org/10.3892/etm.2013.1431
Pages: 355-359

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Abstract

The aim of the present study was to examine the expression of N-cadherin in the myocardial tissues of isoproterenol‑induced myocardial hypertrophy in rats. In addition, the present study provided morphological data to investigate the signal transduction mechanisms of myocardial hypertrophy and reverse myocardial hypertrophy. A myocardial hypertrophy model was established by subcutaneously injecting isoprenaline into healthy adult Sprague‑Dawley rats. The myocardial tissue was collected, embedded in conventional paraffin, sectioned and stained with hematoxylin and the pathological changes were observed. The expression and distribution of N‑cadherin were detected by immunohistochemistry (IHC) and the changes in mRNA expression of N-cadherin in the myocardial tissues of rats were detected by reverse transcription polymerase chain reaction. Image analysis software was used to quantitatively analyze the expression of N‑cadherin. The IHC and immunofluorescence results showed that there was no statistically significant difference between the experimental and control groups in the positive expression of N-cadherin. Furthermore, mRNA expression of N‑cadherin, in the myocardial tissues of rats, was consistent with the IHC and immunofluorescence results. Thus, N‑cadherin may have a significant function in the occurrence and development of myocardial hypertrophy.

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