Expression of lysophosphatidic acid receptors in the human lower esophageal sphincter

Feng,Yong; Liu,Jun‑Feng

doi:10.3892/etm.2013.1439

Expression of lysophosphatidic acid receptors in the human lower esophageal sphincter

Authors:
- Yong Feng
- Jun‑Feng Liu
View Affiliations

Affiliations: Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
Published online on: December 6, 2013 https://doi.org/10.3892/etm.2013.1439
Pages: 423-428

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Lysophosphatidic acid (LPA) is a bioactive lipid that is involved in a variety of physiological and pathological processes occurring in the gastrointestinal tract. It acts via six distinct types of receptors, LPA1, LPA2, LPA3, LPA4, LPA5 and LPA6, which belong to the family of G protein‑coupled receptors. The aim of the present study was to detect the expression of the LPA receptors in the human lower esophageal sphincter (LES). Quantitative polymerase chain reaction and western blotting were used to analyze the expression of LPA1‑6 receptors in sling and clasp fibers from the human LES. The results showed that the protein and mRNA expression levels of various LPA receptors were significantly different. Specifically, the mRNA and protein expression levels of the LPA1 receptor were higher compared with those of the other receptors. The prevalence of the LPA1 receptor mRNA and protein indicates that the LPA1 receptor is likely to be involved in the regulation of human LES functions.

View Figures

View References

1	Eichholtz T, Jalink K, Fahrenfort I and Moolenaar WH: The bioactive phospholipid lysophosphatidic acid is released from activated platelets. Biochem J. 291:677–680. 1993.PubMed/NCBI
2	Tigyi G, Hong L, Yakubu M, Parfenova H, Shibata M and Leffler CW: Lysophosphatidic acid alters cerebrovascular reactivity in piglets. Am J Physiol. 268:H2048–H2055. 1995.PubMed/NCBI
3	Jalink K, Hordijik PL and Moolenaar WH: Growth factor-like effects of lysophosphatidic acid, a novel lipid mediator. Biochim Biophys Acta. 1198:185–196. 1994.PubMed/NCBI
4	Moolenaar WH: Lysophosphatidic acid signaling. Curr Opin Cell Biol. 7:203–210. 1995. View Article : Google Scholar
5	Gaits F, Fourcade O, Le Balle F, et al: Lysophosphatidic acid as a phospholipid mediator: pathways of synthesis. FEBS Lett. 410:54–58. 1997. View Article : Google Scholar : PubMed/NCBI
6	Nietgen G and Durieux ME: Intercellular signaling by lysophosphatidate. Cell Adhes Commun. 5:221–235. 1998. View Article : Google Scholar
7	Ishii I, Contos JJ, Fukushima N and Chun J: Functional comparisons of the lysophosphatidic acid receptors, LP(A1)/VZE-1/EDG-2, LP(A2)/EDG-4, and LP(A3)/EDG-7 in neuronal cell lines using a retrovirus expression system. Mol Pharmacol. 58:895–902. 2000.PubMed/NCBI
8	Moolenaar WH, Van Meeteren LA and Giepmans BN: The ins and outs of lysophosphatidic acid signaling. Bioessays. 26:870–881. 2004. View Article : Google Scholar : PubMed/NCBI
9	Tokumura A, Fukuzawa K and Tsukatani H: Effects of synthetic and natural lysophosphatidic acids in the arterial blood pressure of different animal species. Lipids. 13:572–574. 1978. View Article : Google Scholar : PubMed/NCBI
10	Tokumura A, Fukuzawa K, Yamada S and Tsukatani H: Stimulatory effect of lysophosphatidic acids on uterine smooth muscles of non-pregnant rats. Arch Int Pharmacodyn Ther. 245:74–83. 1980.PubMed/NCBI
11	Tokumura A, Fukuzawa K and Tsukatani H: Contractile actions of lysophosphatidic acids with a chemically-defined fatty acyl group on longitudinal muscle from guineapig ileum. J Pharm Pharmacol. 34:514–516. 1982. View Article : Google Scholar : PubMed/NCBI
12	Tokumura A, Yube N, Fujimoto H and Tsukatani H: Lysophosphatidic acids induce contraction of rat isolated colon by two different mechanisms. J Pharm Pharmacol. 43:774–778. 1991. View Article : Google Scholar : PubMed/NCBI
13	Toews ML, Ustinova EE and Schultz HD: Lysophosphatidic acid enhances contractility of isolated airway smooth muscle. J Appl Physiol. 83:1216–1222. 1997.PubMed/NCBI
14	Hecht JH, Weiner JA, Post SR and Chun J: Ventricular zone gene-1 (vzg-1) encodes a lysophosphatidic acid receptor expressed in neurogenic regions of the developing cerebral cortex. J Cell Biol. 135:1071–1083. 1996. View Article : Google Scholar : PubMed/NCBI
15	An SZ, Bleu T, Hallmark OG and Goetzl EJ: Characterization of a novel subtype of human G protein-coupled receptors for lysophosphatidic acid. J Biol Chem. 273:7906–7910. 1998. View Article : Google Scholar : PubMed/NCBI
16	Bandoh K, Aoki J, Hosono H, et al: Molecular cloning and characterization of a novel human G protein-coupled receptor, EDG7, for lysophosphatidic acid. J Biol Chem. 274:27776–27785. 1999. View Article : Google Scholar : PubMed/NCBI
17	Noguchi K, Ishii S and Shimizu T: Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family. J Biol Chem. 278:25600–25606. 2003. View Article : Google Scholar : PubMed/NCBI
18	Kotarsky K, Boketoft A, Bristulf J, et al: Lysophosphatidic acid binds to and activates GPR92, a G protein-coupled receptor highly expressed in gastrointestinal lymphocytes. J Pharmacol Exp Ther. 318:619–628. 2006. View Article : Google Scholar : PubMed/NCBI
19	Lee CW, Rivera R, Gardell S, Dubin AE and Chun J: GPR92 as a new G(12/13)- and G(q)-coupled lysophosphatidic acid receptor that increases cAMP, LPA5. J Biol Chem. 281:23589–23597. 2006. View Article : Google Scholar : PubMed/NCBI
20	Yanagida K, Masago K, Nakanishi H, Kihara Y, Hamano F, Tajima Y, et al: Identification and characterization of a novel lysophosphatidic acid receptor, p2y5/LPA6. J Biol Chem. 284:17731–17741. 2009. View Article : Google Scholar : PubMed/NCBI
21	Lynch KR and Im DS: Life on the edg. Trends Pharmacol Sci. 20:473–475. 1999. View Article : Google Scholar : PubMed/NCBI
22	Ishii S, Noguchi K and Yanagida K: Non-Edg family lysophosphatidic acid (LPA) receptors. Prostaglandins Other Lipid Mediat. 89:57–65. 2009. View Article : Google Scholar : PubMed/NCBI
23	Yanagida K and Ishii S: Non-Edg family LPA receptors: the cutting edge of LPA research. J Biochem. 150:223–232. 2011. View Article : Google Scholar : PubMed/NCBI
24	Yanagida K, Kurikawa Y, Shimizu T and Ishii S: Current progress in non-Edg family LPA receptor research. Biochim Biophys Acta. 1831:33–41. 2013. View Article : Google Scholar : PubMed/NCBI
25	Lee JW, Kim CH, Wang YY, Yan XM and Sohn UD: Lysophosphatidic acid presynaptically blocks NO uptake during electric field stimulation-induced relaxation via LPA1 receptor in cat lower esophageal sphincter. Arch Pharm Res. 34:169–176. 2011. View Article : Google Scholar : PubMed/NCBI
26	Liu XB and Liu JF: Expression of dopamine receptors in human lower esophageal sphincter. J Gastroenterol Hepatol. 27:945–950. 2012. View Article : Google Scholar : PubMed/NCBI
27	Liu JF, Gao LP, Wen SW, et al: Responses of human sling and clasp fibers to cholecystokinin and gastrin through CCK receptors. J Gastroenterol Hepatol. 23:1608–1612. 2008. View Article : Google Scholar : PubMed/NCBI
28	Liu JF, Lu HL, Wen SW and Wu RF: Effects of acetylcholine on sling and clasp fibers of the human lower esophageal sphincter. J Gastroenterol Hepatol. 26:1309–1317. 2011. View Article : Google Scholar : PubMed/NCBI
29	Sriwai W, Zhou H and Murthy KS: G(q)- dependent signalling by the lysophosphatidic acid receptor LPA(3) in gastric smooth muscle: reciprocal regulation of MYPT1 phosphorylation by Rho kinase and cAMP-independent PKA. Biochem J. 411:543–551. 2008. View Article : Google Scholar : PubMed/NCBI
30	Im DS, Heise CE, Harding MA, George SR, O’Dowd BF, Theodorescu D and Lynch KR: Molecular cloning and characterization of a lysophosphatidic acid receptor, Edg-7, expressed in prostate. Mol Pharmacol. 57:753–759. 2000.PubMed/NCBI