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March 2013 Volume 5 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity

  • Authors:
    • Min Pan
    • Jing Shen
    • Jie Cai

  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Experimental Center of Medical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
  • Pages: 718-722
    |
    Published online on: January 17, 2013
       https://doi.org/10.3892/etm.2013.907
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Abstract

This study demonstrates the removal of virus particles from B95-8 host cells that maintain normal activity under optimal osmotic pressure. After infecting B95-8 cells with Epstein-Barr virus (EBV) particles, the cells were treated with isosmotic solution [0.90% NaCl (330 mOsm/kg H2O)], hyposmotic solutions [0.36% NaCl (115 mOsm/kg H2O) and 0.27% NaCl (93 mOsm/kg H2O)] and distilled water. The pumping levels of virus particles were observed by inverse phase contrast microscopy and transmission electron microscopy (TEM). After treatment with the hyposmotic solutions, the following results were observed: firstly, after culturing for 24 and 48 h, the B95-8 cells in the hyposmotic solutions grew as well as the cells cultured in the isosmotic solution. Secondly, the virus particles in the B95-8 host cells overflowed onto the surface of the cells, while the organelle structures remained intact. This phenomenon was repeated in the removal of human immunodeficiency virus (HIV) from leukomonocytes. By optimizing the osmotic pressure, the activity of the B95-8 host cells was retained and the EBV particles were transported from the cells onto the cell surface.
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Shi LP, Zang YM, Hou XL and Wang J: Ion channel mechanism of regulatory volume decrease in human epithelial cells. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 24:356–360. 2008.(In Chinese).

2. 

Amiji MM and Sandmann BJ: What is osmosis? Explanation and understanding of a physical phenomenon. Applied Physical Pharmacy. McGraw Hill; New York, NY: pp. 54–57. 2002

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Cui Y, Kuang G and Yue H: Chinese herbal medicine for inhibiting EB virus capsid antigen expression in cells in vitro experimental study. Sichuan Cancer Treatment. 14:148–149. 2001.(In Chinese).

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Ling Z: Scanning electron microscopy. Cell Ultrastructure and Electron Microscopy Shanghai Medical. University Press; Shanghai: pp. 124–130. 2000

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Haynie DT: Gibbs free energy -- applications. Biological Thermodynamics. Cambridge University Press; Cambridge: pp. 130–136. 2001

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Copy and paste a formatted citation
Spandidos Publications style
Pan M, Shen J and Cai J: Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity. Exp Ther Med 5: 718-722, 2013.
APA
Pan, M., Shen, J., & Cai, J. (2013). Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity. Experimental and Therapeutic Medicine, 5, 718-722. https://doi.org/10.3892/etm.2013.907
MLA
Pan, M., Shen, J., Cai, J."Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity". Experimental and Therapeutic Medicine 5.3 (2013): 718-722.
Chicago
Pan, M., Shen, J., Cai, J."Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity". Experimental and Therapeutic Medicine 5, no. 3 (2013): 718-722. https://doi.org/10.3892/etm.2013.907
Copy and paste a formatted citation
x
Spandidos Publications style
Pan M, Shen J and Cai J: Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity. Exp Ther Med 5: 718-722, 2013.
APA
Pan, M., Shen, J., & Cai, J. (2013). Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity. Experimental and Therapeutic Medicine, 5, 718-722. https://doi.org/10.3892/etm.2013.907
MLA
Pan, M., Shen, J., Cai, J."Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity". Experimental and Therapeutic Medicine 5.3 (2013): 718-722.
Chicago
Pan, M., Shen, J., Cai, J."Optimizing osmotic pressure removes EBV particles from B95-8 host cells while maintaining normal activity". Experimental and Therapeutic Medicine 5, no. 3 (2013): 718-722. https://doi.org/10.3892/etm.2013.907
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