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April 2013 Volume 5 Issue 4

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Article

Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect

  • Authors:
    • Jörg Warzecha
    • Caroline Seebach
    • Armin Flinspach
    • Florian Wenger
    • Dirk Henrich
    • Ingo Marzi
  • View Affiliations / Copyright

    Affiliations: Department of Trauma Surgery, Johann Wolfgang Goethe University, D‑60590 Frankfurt am Main, Germany
  • Pages: 1035-1039
    |
    Published online on: January 23, 2013
       https://doi.org/10.3892/etm.2013.923
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Abstract

The creation of entirely synthetically derived bone substitute materials which are as effective as autologous bone grafts is desirable. Osteogenesis involves the concerted action of several proteins within a signaling cascade. Hedgehog proteins act upstream of this cascade, inducing the expression of various bone morphogenetic proteins (BMPs) and promoting physiological bone healing. Therefore, the hypothesis that hedgehog signaling in bone defects improves bone healing more than BMP signaling alone was tested. Recombinant N‑terminal sonic hedgehog protein (N‑SHh), BMP‑2 or a combination of the two was added to β‑tricalcium phosphate (β‑TCP) and 5‑mm femoral midshaft defects in nude rats were filled with these composites. The defects were stabilized with mini‑plates. After eight weeks, the animals were sacrificed and the femora were explanted. The radiological evaluation was followed by a three‑point bending test and histological examination. BMP‑2/β‑TCP composites showed a trend of increased stiffness compared with the controls (β‑TCP without protein). N‑SHh/β‑TCP composites had lower stiffness compared with the control group and the N‑SHh/BMP‑2/β‑TCP composites also had lower average stiffness compared with the controls (all not significant). Histomorphometry, however, revealed abundant cartilage and bone core formation in the N‑SHh‑composite groups. The sum of the new cartilage and bone was highest in the combination group N‑SHh/BMP‑2 (not significant). The addition of N‑SHh to bone substitute materials appears to delay bone healing at the applied concentration and observation time but also showed a trend for higher amounts of ossifying cartilage.
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1 

Fietz MJ, Concordet JP, Barbosa R, Johnson R, Krauss S, McMahon AP, Tabin C and Ingham PW: The hedgehog gene family in Drosophila and vertebrate development. Dev Suppl. 3–51. 1994.

2 

Chung UI, Schipani E, McMahon AP and Kronenberg HM: Indian hedgehog couples chondrogenesis to osteogenesis in endochondral bone development. J Clin Invest. 107:295–304. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Day TF and Yang Y: Wnt and hedgehog signaling pathways in bone development. J Bone Joint Surg Am. 90(Suppl 1): 19–24. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Ito H, Akiyama H, Shigeno C, Iyama K, Matsuoka H and Nakamura T: Hedgehog signaling molecules in bone marrow cells at the initial stage of fracture repair. Biochem Biophys Res Commun. 262:443–451. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Spinella-Jaegle S, Rawadi G, Kawai S, Gallea S, Faucheu C, Mollat P, Courtois B, Bergaud B, Ramez V, Blanchet AM, Adelmant G, Baron R and Roman-Roman S: Sonic hedgehog increases the commitment of pluripotent mesenchymal cells into the osteoblastic lineage and abolishes adipocytic differentiation. J Cell Sci. 114:2085–2094. 2001.

6 

Yuasa T, Kataoka H, Kinto N, Iwamoto M, Enomoto-Iwamoto M, Iemura S, Ueno N, Shibata Y, Kurosawa H and Yamaguchi A: Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP-2. J Cell Physiol. 193:225–232. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Enomoto-Iwamoto M, Nakamura T, Aikawa T, Higuchi Y, Yuasa T, Yamaguchi A, Nohno T, Noji S, Matsuya T, Kurisu K, Koyama E, Pacifici M and Iwamoto M: Hedgehog proteins stimulate chondrogenic cell differentiation and cartilage formation. J Bone Miner Res. 15:1659–1668. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Edwards PC, Ruggiero S, Fantasia J, Burakoff R, Moorji SM, Paric E, Razzano P, Grande DA and Mason JM: Sonic hedgehog gene-enhanced tissue engineering for bone regeneration. Gene Ther. 12:75–86. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Urist MR, Lietze A and Dawson E: Beta-tricalcium phosphate delivery system for bone morphogenetic protein. Clin Orthop Relat Res. 187:277–280. 1984.PubMed/NCBI

10 

Tazaki J, Murata M, Akazawa T, Yamamoto M, Ito K, Arisue M, Shibata T and Tabata Y: BMP-2 release and dose-response studies in hydroxyapatite and beta-tricalcium phosphate. Biomed Mater Eng. 19:141–146. 2009.PubMed/NCBI

11 

Garrison KR, Shemilt I, Donell S, Ryder JJ, Mugford M, Harvey I, Song F and Alt V: Bone morphogenetic protein (BMP) for fracture healing in adults. Cochrane Database. Syst Rev. 16:CD0069502010.PubMed/NCBI

12 

Bauer TW and Muschler GF: Bone graft materials. An overview of the basic science. Clin Orthop Relat Res. 371:10–27. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Niemeyer P, Krause U, Fellenberg J, Kasten P, Seckinger A, Ho AD and Simank HG: Evaluation of mineralized collagen and alpha-tricalcium phosphate as scaffolds for tissue engineering of bone using human mesenchymal stem cells. Cells Tissues Organs. 177:68–78. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Schultheiss J, Seebach C, Henrich D, Wilhelm K, Barker JH and Frank J: Mesenchymal stem cell (MSC) and endothelial progenitor cell (EPC) growth and adhesion in six different bone graft substitutes. Eur J Trauma Emerg Surg. 37:635–644. 2011. View Article : Google Scholar

15 

Marigo V, Roberts DJ, Lee SM, Tsukurov O, Levi T, Gastier JM, Epstein DJ, Gilbert DJ, Copeland NG, Seidman CE, et al: Cloning, expression, and chromosomal location of SHH and IHH: two human homologues of the Drosophila segment polarity gene hedgehog. Genomics. 28:44–51. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Ohura K, Hamanishi C, Tanaka S and Matsuda N: Healing of segmental bone defects in rats induced by a beta-TCP-MCPM cement combined with rhBMP-2. J Biomed Mater Res. 44:168–175. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Drosse I, Volkmer E, Seitz S, Seitz H, Penzkofer R, Zahn K, Matis U, Mutschler W, Augat P and Schieker M: Validation of a femoral critical size defect model for orthotopic evaluation of bone healing: a biomechanical, veterinary and trauma surgical perspective. Tissue Eng Part C Methods. 14:79–88. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Niedhart C, Maus U, Redmann E, Schmidt-Rohlfing B, Niethard FU and Siebert CH: Stimulation of bone formation with an in situ setting tricalcium phosphate/rhBMP-2 composite in rats. J Biomed Mater Res A. 65:17–23. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Warzecha J, Seebach C, Flinspach A, Wenger F, Henrich D and Marzi I: Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect. Exp Ther Med 5: 1035-1039, 2013.
APA
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., & Marzi, I. (2013). Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect. Experimental and Therapeutic Medicine, 5, 1035-1039. https://doi.org/10.3892/etm.2013.923
MLA
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., Marzi, I."Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect". Experimental and Therapeutic Medicine 5.4 (2013): 1035-1039.
Chicago
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., Marzi, I."Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect". Experimental and Therapeutic Medicine 5, no. 4 (2013): 1035-1039. https://doi.org/10.3892/etm.2013.923
Copy and paste a formatted citation
x
Spandidos Publications style
Warzecha J, Seebach C, Flinspach A, Wenger F, Henrich D and Marzi I: Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect. Exp Ther Med 5: 1035-1039, 2013.
APA
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., & Marzi, I. (2013). Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect. Experimental and Therapeutic Medicine, 5, 1035-1039. https://doi.org/10.3892/etm.2013.923
MLA
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., Marzi, I."Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect". Experimental and Therapeutic Medicine 5.4 (2013): 1035-1039.
Chicago
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., Marzi, I."Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect". Experimental and Therapeutic Medicine 5, no. 4 (2013): 1035-1039. https://doi.org/10.3892/etm.2013.923
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