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Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect

  • Authors:
    • Jörg Warzecha
    • Caroline Seebach
    • Armin Flinspach
    • Florian Wenger
    • Dirk Henrich
    • Ingo Marzi

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  • Published online on: January 23, 2013     https://doi.org/10.3892/etm.2013.923
  • Pages: 1035-1039
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Abstract

The creation of entirely synthetically derived bone substitute materials which are as effective as autologous bone grafts is desirable. Osteogenesis involves the concerted action of several proteins within a signaling cascade. Hedgehog proteins act upstream of this cascade, inducing the expression of various bone morphogenetic proteins (BMPs) and promoting physiological bone healing. Therefore, the hypothesis that hedgehog signaling in bone defects improves bone healing more than BMP signaling alone was tested. Recombinant N‑terminal sonic hedgehog protein (N‑SHh), BMP‑2 or a combination of the two was added to β‑tricalcium phosphate (β‑TCP) and 5‑mm femoral midshaft defects in nude rats were filled with these composites. The defects were stabilized with mini‑plates. After eight weeks, the animals were sacrificed and the femora were explanted. The radiological evaluation was followed by a three‑point bending test and histological examination. BMP‑2/β‑TCP composites showed a trend of increased stiffness compared with the controls (β‑TCP without protein). N‑SHh/β‑TCP composites had lower stiffness compared with the control group and the N‑SHh/BMP‑2/β‑TCP composites also had lower average stiffness compared with the controls (all not significant). Histomorphometry, however, revealed abundant cartilage and bone core formation in the N‑SHh‑composite groups. The sum of the new cartilage and bone was highest in the combination group N‑SHh/BMP‑2 (not significant). The addition of N‑SHh to bone substitute materials appears to delay bone healing at the applied concentration and observation time but also showed a trend for higher amounts of ossifying cartilage.
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April 2013
Volume 5 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Warzecha J, Seebach C, Flinspach A, Wenger F, Henrich D and Marzi I: Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect. Exp Ther Med 5: 1035-1039, 2013
APA
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., & Marzi, I. (2013). Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect. Experimental and Therapeutic Medicine, 5, 1035-1039. https://doi.org/10.3892/etm.2013.923
MLA
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., Marzi, I."Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect". Experimental and Therapeutic Medicine 5.4 (2013): 1035-1039.
Chicago
Warzecha, J., Seebach, C., Flinspach, A., Wenger, F., Henrich, D., Marzi, I."Effect of sonic hedgehog/β‑TCP composites on bone healing within the critical‑sized rat femoral defect". Experimental and Therapeutic Medicine 5, no. 4 (2013): 1035-1039. https://doi.org/10.3892/etm.2013.923