An animal model of buccal mucosa cancer and cervical lymph node metastasis induced by U14 squamous cell carcinoma cells

Zhao,Xin; Pang,Liang; Qian,Yu; Wang,Qiang; Li,Yong; Wu,Mingyi; Ouyang,Zilan; Gao,Zhi; Qiu,Lihua

doi:10.3892/etm.2013.938

An animal model of buccal mucosa cancer and cervical lymph node metastasis induced by U14 squamous cell carcinoma cells

Authors:
- Xin Zhao
- Liang Pang
- Yu Qian
- Qiang Wang
- Yong Li
- Mingyi Wu
- Zilan Ouyang
- Zhi Gao
- Lihua Qiu
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Affiliations: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China, Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, P.R. China, Department of Stomatology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China
Published online on: January 30, 2013 https://doi.org/10.3892/etm.2013.938
Pages: 1083-1088

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Abstract

The buccal mucosa is the site with the highest risk of contracting a malignancy in habitual betel quid chewers who expose the buccal mucosa to high doses of carcinogens. Of all oral cancers, those of the buccal mucosa are associated with the poorest prognoses. Therefore, it would be helpful to have an animal model to evaluate new treatment modalities for buccal mucosa cancer. In the present study, we evaluated whether the imprinting control region (ICR) mouse animal model could be employed as a cancer model for buccal mucosa cancer. Sixty male ICR mice were randomly divided into two groups, a normal group (n=10) and a cancer-induced group (n=50). Each mouse in the cancer group was inoculated with 0.05 ml U14 cancer cell suspension (1x107/ml) on the buccal mucosa. Histological staining and gene expression assays revealed that neck lymph node metastasis animal models were established. After 20 days, the cheek tumor formation rate of the ICR mice reached 100%. Furthermore, the neck lymph node metastasis rate was 53%. We identified that U14 cells produce strong metastasis in ICR mice. Metastasis of the tumor to the lymph node began with carcinoma metastasis encroaching on the marginal sinus. Then it infiltrated to the cortex and medulla and the infiltration continued until the normal lymph node structure was completely damaged. This animal model may be employed in medical research on buccal mucosa cancer and cervical lymph node metastasis. In conclusion, our findings indicate that U14 cell-induced mouse buccal mucosa cancer may be a potential cancer model for human buccal mucosa squamous cell carcinoma.

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1	Chaturvedi AK, Engels EA, Anderson WF and Gillison ML: Incidence trends for human papillomavirus-related and -unrelated oral squamous cell carcinomas in the United States. J Clin Oncol. 26:612–619. 2008. View Article : Google Scholar : PubMed/NCBI
2	Hong WK, Lippman SM, Itri LM, Karp DD, Lee JS, Byers RM, Schantz SP, Kramer AM, Lotan R, Peters LJ, Dimery IW, Brown BW and Goepfert H: Prevention of second primary tumors with isotretinoin in squamous-cell carcinoma of the head and neck. New Engl J Med. 323:795–801. 1990. View Article : Google Scholar : PubMed/NCBI
3	Alam M and Ratner D: Cutaneous squamous-cell carcinoma. New Engl J Med. 344:975–983. 2001. View Article : Google Scholar : PubMed/NCBI
4	Duvoux C, Delacroix I, Richardet JP, Roudot-Thoraval F, et al: Increased incidence of oropharyngeal squamous cell carcinomas after liver transplantation for alcoholic cirrhosis. Transplantation. 67:418–421. 1999. View Article : Google Scholar : PubMed/NCBI
5	Mognetti B, Carlo FD and Berta GN: Animal models in oral cancer research. Oral Oncol. 42:448–460. 2006. View Article : Google Scholar : PubMed/NCBI
6	Gu B, Feng HL, Dong JH, Zhang H, Bian XC and Liu YQ: The establishment and characterization of a continuous cell line of mouse cervical carcinoma. Chin J Clin Oncol. 5:44–48. 2008. View Article : Google Scholar
7	Liu XF, Ren LR, Su GY, Liu YQ, Gu B and Dong JH: Establishment and characterization of a rabbit tumor cell line VX2. Chinese J Pathol. 34:661–663. 2005.(In Chinese).
8	Su GY, Liu XF, Ren LR, Gu P, Zhang JB and Liu YQ: Establishment and characterization of a continuous cell line of mouse breast cancer. Chinese J Clin Oncol. 33:1150–1152. 2006.
9	Rattanasinganchan P, Leelawat K, Treepongkaruna SA, Tocharoentanaphol C, Subwongcharoen S, Suthiphongchai T and Tohtong R: Establishment and characterization of a cholangio-carcinoma cell line (RMCCA-1) from a Thai patient. World J Gastroenterol. 12:6500–6506. 2006.PubMed/NCBI
10	Schrader M and Laberke HG: Differential diagnosis of verrucous carcinoma in the oral cavity and larynx. J Laryngol Otol. 102:700–703. 1988. View Article : Google Scholar : PubMed/NCBI
11	Bak SS, Kong CS, Rhee SH, Rho CW, Kim NK, Choi KL and Park KY: Effect of sulfur enriched young radish kimchi on the induction of apoptosis in AGS human gastric adenocarcinoma cells. J Food Sci Nutr. 12:79–83. 2007. View Article : Google Scholar
12	Kim YA, Rhee SH, Park KY and Choi YH: Antiproliferative effect of resveratrol in human prostate carcinoma cells. J Med Food. 6:273–280. 2003. View Article : Google Scholar : PubMed/NCBI
13	Gao J and Zhang JB: Tumor Invasion and Metastasis. Science Publishing House; Beijing: pp. 531–532. 2003
14	Xuan M, Weng YM, Wang CM and Li XQ: Pathologic changes of lymphatic capillaries after inoculation of U14 cells in rat tongue perineoplastic area. West China J Stomatol. 18:5–8. 2000.PubMed/NCBI
15	Milanezi F, Leitão D, Ricardo S, Augusto I and Schmitt F: Evaluation of HER2 in breast cancer: reality and expectations. Expert Opin Med Diagn. 3:607–620. 2009. View Article : Google Scholar : PubMed/NCBI
16	Chao DT and Korsmeyer SJ: Bcl-2 family: regulators of cell death. Annu Rev Immunol. 16:395–419. 1998. View Article : Google Scholar
17	Blanc C, Deveraux QL, Krajewski S, Jänicke RU, Porter AG, Reed JC, Jaggi R and Marti A: Caspase-3 is essential for procaspase-9 processing and cisplatin-induced apoptosis of MCF-7 breast cancer cells. Cancer Res. 60:4386–4390. 2000.PubMed/NCBI
18	Kirsch DG, Doseff A, Chau BN, Lim DS, de Souza-Pinto NC, Hansford R, Kastan MB, Lazebnik YA and Hardwick JM: Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c. J Biol Chem. 274:21155–21161. 1999. View Article : Google Scholar : PubMed/NCBI
19	Delić R and Štefanović M: Optimal laboratory panel for predicting preeclampsia. J Maternal-Fetal Neonatal Med. 23:96–102. 2010.PubMed/NCBI
20	Baeuerle PA: IkappaB-NF-kappaB structures: at the interface of inflammation control. Cell. 95:729–731. 1998. View Article : Google Scholar : PubMed/NCBI
21	Klein CA: Cancer. The metastasis cascade Science. 321:1785–1787. 2008.PubMed/NCBI
22	Vihinen P, Ala-Aho R and Kahari VM: Matrix metalloproteinase as therapeurtic targets in cancer. Curr Cancer Drug Targets. 5:203–220. 2005. View Article : Google Scholar : PubMed/NCBI
23	Davies B, Waxman J, Wasan H, Abel P, Williams G, Krausz T, Neal D, Thomas D, Hanby A and Balkwill F: Levels of matrix metalloproteinase in bladder cancer correlate with tumor grade and invasion. Cancer Res. 53:5365–5369. 1993.PubMed/NCBI
24	Chen PN, Chu SC, Chiou HL, Kuo WH, Chiang CL and Hsieh YS: Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line. Cancer Lett. 235:248–259. 2006. View Article : Google Scholar : PubMed/NCBI
25	Uzui H, Harpf A, Liu M, Dogerry TM, Shukla A and Chai N: Increased expression of membrane type 3-matrix metalloproteinase in human atherosclerotic plaque: role of activated macrophage and inflammatory cytokines. Circulation. 106:3024–3030. 2002. View Article : Google Scholar : PubMed/NCBI
26	Lambert E, Dasse E, Haye B and Petitfrere E: TIMPs as multi-facial proteins. Crit Rev Oncol Hematol. 49:187–198. 2004. View Article : Google Scholar : PubMed/NCBI
27	Deryugina EI and Quigley JP: Matrix metalloproteinases and tumor metastasis. Cancer Metastasis Rev. 25:9–34. 2006. View Article : Google Scholar
28	Krüger A, Fata JE and Khokha R: Altered tumor growth and metastasis of a T-cell lymphoma in TIMP-1 transgenic mice. Blood. 90:1993–2000. 1997.PubMed/NCBI
29	Krüger A, Sanchez-Sweatman OH, Martin DC, Fata JE, Ho AT, Orr FW, Rüther U and Khokha R: Host TIMP-1 overexpression confers resistance to experimental brain metastasis of a fibrosarcoma cell line. Oncogene. 16:2419–2423. 1998.PubMed/NCBI
30	Liu S, Zhao F and Ben C: Evaluation of clinical and pathological factors relating to lymph node metastasis in carcinoma of the tongue. Zhonghua Kou Qiang Yi Xue Za Zhi. 31:259–262. 1996.(In Chinese).