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Article

Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver

  • Authors:
    • Y. S. Tian
    • T. Z. Rong
    • Y. L. Hong
    • L. Min
    • P. G. Jian
  • View Affiliations / Copyright

    Affiliations: Chongqing Key Laboratory of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing, P.R. China, Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing, P.R. China
  • Pages: 1169-1173
    |
    Published online on: January 31, 2013
       https://doi.org/10.3892/etm.2013.941
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Abstract

It has been demonstrated that ischemic postconditioning (IPO) is capable of attenuating ischemia/reperfusion (I/R) injury in the heart. However, the novel role of pharmacological postconditioning in the liver remains unclear. In this study, the hypothesis that diazoxide postconditioning reduces I/R‑induced injury in rat liver was tested. Rats were assigned randomly to the sham‑operated control, I/R (occlusion of the porta hepatis for 60 min, followed by a persistent reperfusion for 120 min), diazoxide ischemic postconditioning (DIPO; occlusion of the porta hepatis for 60 min, then treatment with diazoxide for 10 min reperfusion, followed by a persistent reperfusion for 110 min) or 5‑hydroxydecanoate (5‑HD)+DIPO (occlusion of the porta hepatis for 60 min, then treatment with diazoxide and 5‑HD for 10 min reperfusion, followed by a persistent reperfusion for 110 min) groups. The alanine aminotransferase (ALT) and aspartate transaminase (AST) levels were assayed. The expression levels of protein kinase c‑ε (pkc‑ε), cytochrome c (cyt‑c), caspase‑3 and bcl‑2 protein were determined by western blotting. The serum levels of ALT and AST and expression levels of cyt‑c and caspase‑3 were significantly lower in the DIPO group (P<0.05). However, the protein expression levels of pkc‑ε and bcl‑2 were markedly increased in the DIPO group (P<0.05). 5‑HD abrogated the protective effects of DIPO. The data of the present study provide the first evidence that DIPO protects the liver from I/R injury by opening the mitochondrial KATP channels, activating and upregulating pkc‑ε and inhibiting the activation of the apoptotic pathway by decreasing the release of cyt‑c and the expression of caspase‑3 and increasing bcl‑2 expression.
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1 

Murry CE, Jennings RB and Reimer KA: Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation. 74:1124–1136. 1986. View Article : Google Scholar

2 

Hausenloy DJ, Mocanu MM and Yellon DM: Cross-talk between the survival kinases during early reperfusion: its contribution to ischemic preconditioning. Cardiovasc Res. 63:305–312. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Liu GS, Thornton J, Van Winkle DM, et al: Protection against infarction afforded by preconditioning is mediated by A1 adenosine receptors in rabbit heart. Circulation. 84:350–356. 1991. View Article : Google Scholar : PubMed/NCBI

4 

Leesar MA, Stoddard M, Ahmed M, et al: Preconditioning of human myocardium with adenosine during coronary angioplasty. Circulation. 95:2500–2507. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Costa AD, Garlid KD, West IC, et al: Protein kinase G transmits the cardioprotective signal from cytosol to mitochondria. Circ Res. 97:329–336. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Tsang A, Hausenloy DJ, Mocanu MM and Yellon DM: Postconditioning: a form of ‘modified reperfusion’ protects the myocardium by activating the phosphatidylinositol 3-Kinase-Akt pathway. Circ Res. 95:230–232. 2004.

7 

Darling CE, Jiang R, Maynard M, et al: Postconditioning via stuttering reperfusion limits myocardial infarct size in rabbit hearts: role of Erk1/2. Am J Physiol Heart Circ Physiol. 289:H1618–H1626. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Zhao ZQ, Corvera JS, Halkos ME, et al: Inhibition of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning. Am J Physiol Heart Circ Physiol. 285:H579–H588. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Heusch G: Postconditioning: old wine in a new bottle? J Am Coll Cardiol. 44:1111–1112. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Vinten-Johansen J, Yellon DM and Opie LH: Postconditioning: a simple clinically applicable procedure to improve revascularization in acute myocardial infarction. Circulation. 112:2085–2088. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Jabúrek M, Costa AD, Burton JR, et al: Mitochondrial PKC epsilon and mitochondrial ATP-sensitive K+ channel copurify and coreconstitute to form a functioning signaling module in proteoliposomes. Circ Res. 99:878–883. 2006.PubMed/NCBI

12 

Costa AD, Jakob R, Costa CL, et al: The mechanism by which the mitochondrial ATP-sensitive K+ channel opening and H2O2 inhibit the mitochondrial permeability transition. J Biol Chem. 281:20801–20808. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Dai AL, Fan LH, Zhang FJ, et al: Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury. J Zhejiang Univ Sci B. 11:267–274. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Fu XC, Wang MW, Li SP and Wang HL: Anti-apoptotic effect and the mechanism of orientin on ischaemic/reperfused myocardium. J Asian Nat Prod Res. 8:265–272. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Ferrer I and Planas AM: Signaling of cell death and cell survival following focal cerebral ischemia: life and death struggle in the penumbra. J Neuropathol Exp Neurol. 62:329–339. 2003.PubMed/NCBI

16 

Fryer RM, Eells JT, Hsu AK, et al: Ischemic preconditioning in rats: role of mitochondrial KATP channel in preservation of mitochondrial function. Am J Physiol Heart Circ Physiol. 278:H305–H312. 2000.PubMed/NCBI

17 

Liu Y, Sato T, Seharaseyson J, et al: Mitochondrial ATP-dependent potassium channels. Viable candidate effectors of ischemic preconditioning. Ann NY Acad Sci. 874:27–37. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Garlid KD, Paucek P, Yarov-Yarovoy V, et al: The mitochondrial KATP channel as a receptor for potassium channel openers. J Biol Chem. 271:8796–8799. 1996.PubMed/NCBI

19 

Dos Santos P, Kowaltowski AJ, Laclau MN, et al: Mechanisms by which opening the mitochondrial ATP-sensitive K+ channel protects the ischemic heart. Am J Physiol Heart Circ Physiol. 283:H284–H295. 2002.PubMed/NCBI

20 

Kang PM, Haunstetter A, Aoki H, et al: Morphological and molecular characterization of adult cardiomyocyte apoptosis during hypoxia and reoxygenation. Circ Res. 87:118–125. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Zhang H, Li Q, Li Z, et al: The protection of bcl-2 overexpression on rat cortical neuronal injury caused by analogous ischemia/reperfusion in vitro. Neurosci Res. 62:140–146. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Yang J, Wang J, Zhu S, et al: C-reactive protein augments hypoxia-induced apoptosis through mitochondrion-dependent pathway in cardiac myocytes. Mol Cell Biochem. 310:215–226. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Inagaki K, Hahn HS, Dorn GW II, et al: Additive protection of the ischemic heart ex vivo by combined treatment with delta-protein kinase c inhibitor and epsilon-protein kinase C activator. Circulation. 108:869–875. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Inagaki K, Chen L, Ikeno F, et al: Inhibition of delta-protein kinase C protects against reperfusion injury of the ischemic heart in vivo. Circulation. 108:2304–2307. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Bright R, Raval AP, Dembner JM, et al: Protein kinase C delta mediates cerebral reperfusion injury in vivo. J Neurosci. 24:6880–6888. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Zatta AJ, Kin H, Lee G, et al: Infarct-sparing effect of myocardial postconditioning is dependent on protein kinase c signalling. Cardiovasc Res. 70:315–324. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Churchill EN and Szweda LI: Translocation of deltaPKC to mitochondria during cardiac reperfusion enhances superoxide anion production and induces loss in mitochondrial function. Arch Biochem Biophys. 439:194–199. 2005. View Article : Google Scholar

28 

Murriel CL, Churchill E, Inagaki K, et al: Protein kinase Cdelta activation induces apoptosis in response to cardiac ischemia and reperfusion damage: a mechanism involving BAD and the mitochondria. J Biol Chem. 279:47985–47991. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Liu GS, Cohen MV, Mochly-Rosen D and Downey JM: Protein kinase C-epsilon is responsible for the protection of preconditioning in rabbit cardiomyocytes. J Mol Cell Cardiol. 31:1937–1948. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Toma O, Weber NC, Wolter JI, et al: Desflurane preconditioning induces time-dependent activation of protein kinase C epsilon and extracellular signal-regulated kinase 1 and 2 in the rat heart in vivo. Anesthesiology. 101:1372–1380. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Ludwig LM, Weihrauch D, Kersten JR, et al: Protein kinase C translocation and Src protein tyrosine kinase activation mediate isoflurane-induced preconditioning in vivo: potential downstream targets of mitochondrial adenosine triphosphate-sensitive potassium channels and reactive oxygen species. Anesthesiology. 100:532–539. 2004. View Article : Google Scholar

32 

Ping P, Zhang J, Qiu Y, et al: Ischemic preconditioning induces selective translocation of protein kinase C isoforms epsilon and eta in the heart of conscious rabbits without subcellular redistribution of total protein kinase C activity. Circ Res. 81:404–414. 1997. View Article : Google Scholar

33 

Novalija E, Kevin LG, Camara AKS, et al: Reactive oxygen species precede the epsilon isoform of protein kinase C in the anesthetic preconditioning signaling cascade. Anesthesiology. 99:421–428. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Qiu Y, Ping P, Tang XL, et al: Direct evidence that protein kinase C plays an essential role in the development of late preconditioning against myocardial stunning in conscious rabbits and that epsilon is the isoform involved. J Clin Invest. 101:2182–2198. 1998. View Article : Google Scholar

35 

Yang Z, Sun W and Hu K: Molecular mechanism underlying adenosine receptor-mediated mitochondrial targeting of protein kinase C. Biochim Biophys Acta. 1823:950–958. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Kaljusto ML, Rutkovsky A, Stensløkken KO, et al: Postconditioning in mouse hearts is inhibited by blocking the reverse mode of the sodium-calcium exchanger. Interact Cardiovasc Thorac Surg. 10:743–748. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Jones WK, Fan GC, Liao S, et al: Peripheral nociception associated with surgical incision elicits remote nonischemic cardioprotection via neurogenic activation of protein kinase C signaling. Circulation. 120(Suppl 11): 1–9. 2009. View Article : Google Scholar

38 

Liu H, Zhang HY, Zhu X, et al: Preconditioning blocks cardiocyte apoptosis: role of K+ATP channels and PKCε. Am J Physiol Heart Circ Physiol. 282:1380–1386. 2002. View Article : Google Scholar : PubMed/NCBI

39 

McPherson BC and Yao Z: Morphine mimics preconditioning via free radical signals and mitochondrial KATP channels in myocytes. Circulation. 103:290–295. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tian YS, Rong TZ, Hong YL, Min L and Jian PG: Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver. Exp Ther Med 5: 1169-1173, 2013.
APA
Tian, Y.S., Rong, T.Z., Hong, Y.L., Min, L., & Jian, P.G. (2013). Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver. Experimental and Therapeutic Medicine, 5, 1169-1173. https://doi.org/10.3892/etm.2013.941
MLA
Tian, Y. S., Rong, T. Z., Hong, Y. L., Min, L., Jian, P. G."Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver". Experimental and Therapeutic Medicine 5.4 (2013): 1169-1173.
Chicago
Tian, Y. S., Rong, T. Z., Hong, Y. L., Min, L., Jian, P. G."Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver". Experimental and Therapeutic Medicine 5, no. 4 (2013): 1169-1173. https://doi.org/10.3892/etm.2013.941
Copy and paste a formatted citation
x
Spandidos Publications style
Tian YS, Rong TZ, Hong YL, Min L and Jian PG: Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver. Exp Ther Med 5: 1169-1173, 2013.
APA
Tian, Y.S., Rong, T.Z., Hong, Y.L., Min, L., & Jian, P.G. (2013). Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver. Experimental and Therapeutic Medicine, 5, 1169-1173. https://doi.org/10.3892/etm.2013.941
MLA
Tian, Y. S., Rong, T. Z., Hong, Y. L., Min, L., Jian, P. G."Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver". Experimental and Therapeutic Medicine 5.4 (2013): 1169-1173.
Chicago
Tian, Y. S., Rong, T. Z., Hong, Y. L., Min, L., Jian, P. G."Pharmacological postconditioning with diazoxide attenuates ischemia/reperfusion‑induced injury in rat liver". Experimental and Therapeutic Medicine 5, no. 4 (2013): 1169-1173. https://doi.org/10.3892/etm.2013.941
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