An altered expression of genes involved in the regulation of ion channels in atrial myocytes is correlated with the risk of atrial fibrillation in patients with heart failure

Gao,Mei; Wang,Jiangrong; Wang,Zhongsu; Zhang,Yong; Sun,Hui; Xie,Xinxing; Hou,Yinglong

doi:10.3892/etm.2013.949

April 2013 Volume 5 Issue 4

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April 2013 Volume 5 Issue 4

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Article

An altered expression of genes involved in the regulation of ion channels in atrial myocytes is correlated with the risk of atrial fibrillation in patients with heart failure

Authors:
- Mei Gao
- Jiangrong Wang
- Zhongsu Wang
- Yong Zhang
- Hui Sun
- Xinxing Xie
- Yinglong Hou
View Affiliations / Copyright

Affiliations: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, P.R. China
Pages: 1239-1343
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Published online on: February 5, 2013

https://doi.org/10.3892/etm.2013.949
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Abstract

The aim of this study was to investigate the correlation between the altered expression of genes involved in the regulation of ion channels in atrial myocytes and the risk of atrial fibrillation (AF) in patients with heart failure (HF). Right atrial appendages were obtained from 18 HF patients and 18 patients with normal cardiac functions who had undergone surgery. The mRNA expression levels of Kv4.3α, KvLQT1, Kv1.5, L-Caα1c and NCX were measured by reverse transcription-PCR (RT-PCR). Protein expression levels were also detected by western blotting. In comparison with the control group exhibiting normal cardiac functions, the mRNA and protein expression levels of Kv4.3α, KvLQT1 and L-Caα1c were significantly reduced in HF patients. By contrast, the mRNA and protein expression levels of NCX were significantly increased in HF patients compared with the control group (P<0.01). The mRNA expression levels of Kv1.5 were not evidently altered. We demonstrated that increased levels of Kv4.3α, KvLQT1 and L-Caα1c and decreased levels of NCX are correlated with the risk of AF in HF patients. Changes in the gene expression of ion channel-related proteins may therefore be used as biological markers of AF occurring in HF patients in future studies.

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1	Grönefeld GC and Hohnloser SH: Heart failure complicated by atrial fibrillation: mechanistic, prognostic, and therapeutic implications. J Cardiovasc Pharmacol Ther. 8:107–113. 2003.PubMed/NCBI
2	Franz MR, Karasik PL, Li C, Moubarak J and Chavez M: Electrical remodeling of the human atrium: similar effects in patients with chronic atrial fibrillation and atrial flutter. J Am Coll Cardiol. 30:1785–1792. 1997. View Article : Google Scholar : PubMed/NCBI
3	Pandozi C, Bianconi L, Villani M, et al: Electrophysiological characteristics of the human atria after cardioversion of persistent atrial fibrillation. Circulation. 98:2860–2865. 1998. View Article : Google Scholar : PubMed/NCBI
4	Yue L, Melnyk P, Gaspo R, Wang Z and Nattel S: Molecular mechanisms underlying ionic remodeling in a dog model of atrial fibrillation. Circ Res. 84:776–784. 1999. View Article : Google Scholar : PubMed/NCBI
5	Van Wagoner DR, Pond AL, Lamorgese M, Rossie SS, McCarthy PM and Nerbonne JM: Atrial L-type Ca2+ currents and human atrial fibrillation. Circ Res. 84:428–436. 1999.
6	Krogh-Madsen T, Abbott GW and Christini DJ: Effects of electrical and structural remodeling on atrial fibrillation maintenance: a simulation study. PLoS Comput Biol. 8:e10023902012. View Article : Google Scholar : PubMed/NCBI
7	Hunter RJ, Liu Y, Lu Y, Wang W and Schilling RJ: Left atrial wall stress distribution and its relationship to electrophysiologic remodeling in persistent atrial fibrillation. Circ Arrhythm Electrophysiol. 5:351–630. 2012. View Article : Google Scholar : PubMed/NCBI
8	Zhang Y, Dong D and Yang B: Atrial remodeling in atrial fibrillation and association between microRNA network and atrial fibrillation. Sci China Life Sci. 54:1097–1102. 2011. View Article : Google Scholar : PubMed/NCBI
9	Pang H, Ronderos R, Pérez-Riera AR, Femenía F and Baranchuk A: Reverse atrial electrical remodeling: a systematic review. Cardiol J. 18:625–631. 2011. View Article : Google Scholar : PubMed/NCBI
10	Li D, Melnyk P, Feng J, Wang Z, Petrecca K, Shrier A and Nattel S: Effects of experimental heart failure on atrial cellular and ionic electrophysiology. Circulation. 101:2631–2638. 2000. View Article : Google Scholar : PubMed/NCBI
11	Cha TJ, Ehrlich JR, Zhang L, Shi YF, Tardif JC, Leung TK and Nattel S: Dissociation between ionic remodeling and ability to sustain atrial fibrillation during recovery from experimental congestive heart failure. Circulation. 109:412–418. 2004. View Article : Google Scholar
12	Cha TJ, Ehrlich JR, Zhang L and Nattel S: Atrial ionic remodeling induced by atrial tachycardia in the presence of congestive heart failure. Circulation. 110:1520–1526. 2004. View Article : Google Scholar : PubMed/NCBI
13	Kääb S, Nuss HB, Chiamvimonvat N, et al: Ionic mechanism of action potential prolongation in ventricular myocytes from dogs with pacing-induced heart failure. Circ Res. 78:262–273. 1996.
14	Mankad S, d’Amato TA, Reichek N, et al: Combined angiotensin II receptor antagonism and angiotensin-converting enzyme inhibition further attenuates postinfarction left ventricular remodeling. Circulation. 103:2845–2850. 2001. View Article : Google Scholar
15	Charpentier F, Mérot J, Loussouarn G and Baró I: Delayed rectifier K(+) currents and cardiac repolarization. J Mol Cell Cardiol. 48:37–44. 2010.
16	Thomas D, Wimmer AB, Karle CA, et al: Dominant-negative I(Ks) suppression by KCNQ1-deltaF339 potassium channels linked to Romano-Ward syndrome. Cardiovasc Res. 67:487–497. 2005. View Article : Google Scholar : PubMed/NCBI
17	Xu X, Rials SJ, Wu Y, et al: Left ventricular hypertrophy decreases slowly but not rapidly activating delayed rectifier potassium currents of epicardial and endocardial myocytes in rabbits. Circulation. 103:1585–1590. 2001. View Article : Google Scholar
18	Pogwizd SM, Schlotthauer K, Li L, Yuan W and Bers DM: Arrhythmogenesis and contractile dysfunction in heart failure: Roles of sodium-calcium exchange, inward rectifier potassium current, and residual beta-adrenergic responsiveness. Circ Res. 88:1159–1167. 2001. View Article : Google Scholar
19	Pritchard TJ and Kranias EG: Junctin and the histidine-rich Ca2+ binding protein: potential roles in heart failure and arrhythmogenesis. J Physiol. 587:3125–3133. 2009.PubMed/NCBI
20	George CH: Sarcoplasmic reticulum Ca2+ leak in heart failure: mere observation or functional relevance? Cardiovasc Res. 77:302–314. 2008.
21	Stambler BS, Fenelon G, Shepard RK, Clemo HF and Guiraudon CM: Characterization of sustained atrial tachycardia in dogs with rapid ventricular pacing-induced heart failure. J Cardiovasc Electrophysiol. 14:499–507. 2003. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

An altered expression of genes involved in the regulation of ion channels in atrial myocytes is correlated with the risk of atrial fibrillation in patients with heart failure

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