Characteristics of the four subtypes of myelodysplastic/myeloproliferative neoplasms

Wu,Huanling; Bian,Shuquan; Chu,Jingxue; Zhong,Xiaoyan; Sun,Hui; Zhang,Bingchang; Lu,Zhiming

doi:10.3892/etm.2013.975

May 2013 Volume 5 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May 2013 Volume 5 Issue 5

Full Size Image

Article

Characteristics of the four subtypes of myelodysplastic/myeloproliferative neoplasms

Authors:
- Huanling Wu
- Shuquan Bian
- Jingxue Chu
- Xiaoyan Zhong
- Hui Sun
- Bingchang Zhang
- Zhiming Lu
View Affiliations / Copyright

Affiliations: Clinical Laboratory of Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Clinical Laboratory of Xintai Hospital Affiliated to Taishan Medical University, Jinan, Shandong 271200, P.R. China, Clinical Laboratory of Jinan Central Hospital, Jinan, Shandong 250013, P.R. China, Clinical Laboratory of the Eighth Hospital in Jinan, Jinan, Shandong 250014, P.R. China, Shandong Center For Clinical Laboratories, Jinan, Shandong 250021, P.R. China
Pages: 1332-1338
|
Published online on: February 26, 2013

https://doi.org/10.3892/etm.2013.975
Expand metrics +

Abstract

The aim of the present study was to investigate the characteristics of the four subtypes of myelodysplastic/myeloproliferative neoplasms (MDS/MPNs) in order to improve current knowledge and to aid their diagnosis. A total of 53 cases of MDS/MPNs were analyzed using routine blood cell analysis and morphological, cytogenetic and molecular genetic characteristics were investigated. Numerical data for several groups were compared using a single-factor analysis of variance. The Student-Newman‑Keuls test was used to compare the means of two groups. The proportions were compared using a Chi-square test or Fisher's exact test. Analysis of the patients with MDS/MPNs revealed that 46 patients (86.8%) had paleness and fatigue, and blood analysis revealed hemoglobin (Hb) levels of 83.1±24.6 g/l, a white blood cell (WBC) count of 19.8±8.1x109/l and a platelet (PLT) count of 158.7±108.2x1012/l. Immature neutrophils and monocytes were identified in the peripheral blood at levels of 0.058±0.031 and 0.152±0.034%, respectively. There were 23 cases (43.4%) with dyserythropoiesis and 36 cases (67.9%) had dysgranulopoiesis. Fifteen cases were immunologically characterized using flow cytometry (FCM), of which 13 cases showed abnormalities on blasts and myelocytes. Karyotyping was performed in 27 cases of MDS/MPN and 12 (44.4%) were identified as abnormal. In 23 cases, testing for BCR/ABL1, AML-ETO, CBF-MYH11A, PML-RARA, E2A-PBX1, TEL-AML1, SIL-TAL1 returned negative results. The JAK2V617F mutation was positive in one of five cases. The majority of MDS/MPN cases had anemia, cytosis, low-grade blasts and immature neutrophils in the peripheral blood and dysplasia in the bone marrow. Immunological abnormalities and abnormal karyotypes occurred frequently in MDS/MPNs and although there were no statistical differences between the four subtypes, these were able to aid diagnosis. No specific molecular abnormalities were identified in MDS/MPNs.

View Figures

View References

1.	Swerdlow SH, Campo E, Harris NL, et al: WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. 4th edition. IARC Press; Lyon, France: pp. 18–30. 2008
2.	Gong XB, Lu XG, Wu XG, et al: Morphological and histopathological characteristics of atypical chronic myeloid leukemia. Zhonghua Xue Ye Xue Za Zhi. 30:563–566. 2009.(In Chinese).
3.	Ma Y, Xu XP, Wang XQ, et al: Clinical analysis of eight adult MDS/MPNs-U patients. China J Hematol. 30:351–353. 2009.(In Chinese).
4.	Deng JD: Clinical Hematology. Shanghai Scienctific Technology Publishing House. 132–137. 2001.
5.	Gong XB, Lu XG, Yan LJ, et al: Staining technology of micro-megakaryocyte and its clinical value. Zhonghua Xue Ye Xue Za Zhi. 6:420–422. 2008.
6.	Stachurski D, Smith BR, Pozdnyakova O, et al: Flow cytometric analysis of myelomonocytic cells by a pattern recognition approach is sensitive and specific in diagnosing myelodysplastic syndrome and related marrow diseases: emphasis on a global evaluation and recognition of diagnostic pitfalls. Leuk Res. 32:215–224. 2008. View Article : Google Scholar
7.	Wu HJ, Shen WZ, Li YQ, et al: Clinical analysis of 16 chronical myelomonocytic leukemia patients. Di 3 Jun Yi Da Xue Xue Bao. 30:1391–1392. 2008.(In Chinese).
8.	Wang SA, Galili N, Cerny J, et al: Chronic myelomonocytic leukemia evolving from preexisting myelodysplasia shares many features with de novo disease. Am J Clin Pathol. 126:789–797. 2006. View Article : Google Scholar : PubMed/NCBI
9.	Onida F, Ball G, Kantarjian HM, et al: Characteristics and outcome of patients with Philadelphia chromosome negative, bcr/abl negative chronic myelogenous leukemia. Cancer. 95:1673–1684. 2002. View Article : Google Scholar : PubMed/NCBI
10.	Luna-Fineman S, Shannon KM, Atwater SK, et al: Myelodysplastic and myeloproliferative disorders of childhood: a study of 167 patients. Blood. 93:459–466. 1999.PubMed/NCBI
11.	Lu FJ, Zhai XW, Gao YJ, et al: Clinical analysis and treatment of juvenile myelomonocytic leukemia. Lin Chuang Er Ke Za Zhi. 25:648–650. 2007.(In Chinese).
12.	Hernández JM, Del Cañizo MC, Cuneo A, et al: Clinical, hematological and cytogenetic characteristics of atypical chronic myeloid leukemia. Ann Oncol. 11:441–444. 2000.
13.	Atallah E, Nussenzveig R, Yin CC, et al: Prognostic interaction between thrombocytosis and JAK2 V617F mutation in the WHO subcategories of myelodysplastic/myeloproliferative disease-unclassifiable and refractory anemia with ringed sidero-blasts and marked thrombocytosis. Leukemia. 22:1295–1298. 2008. View Article : Google Scholar
14.	Wang SA, Hasserjian RP, Loew JM, et al: Refractory anemia with ringed sideroblasts associated with marked thrombocytosis harbors JAK2 mutation and shows overlapping myeloproliferative and myelodysplastic features. Leukemia. 20:1641–1644. 2006. View Article : Google Scholar
15.	Yang WY, Zhao YX, Chen XJ, et al: Analysis of clinical and biological characteristics of myeloplastic and myeloproliferative disorders in children. J China Pediatr Blood Cancer. 14:105–107. 2009.(In Chinese).
16.	Koike K and Matsuda K: Recent advances in the pathogenesis and management of juvenile myelomonocytic leukaemia. Br J Haematol. 141:567–575. 2008. View Article : Google Scholar : PubMed/NCBI
17.	Jones AV, Kreil S, Zoi K, et al: Widespread occurrence of the JAK2 V617F mutation in chronic myeloproliferative disorders. Blood. 106:2162–2168. 2005. View Article : Google Scholar : PubMed/NCBI
18.	Reiter A, Invernizzi R, Cross NC and Cazzola M: Molecular basis of myelodysplastic/myeloproliferative neoplasms. Haematologica. 94:1634–1638. 2009. View Article : Google Scholar : PubMed/NCBI
19.	Tefferi A: Novel mutations and their functional and clinical relevance in myeloproliferative neoplasms: JAK2, MPL, TET2, ASXL1, CBL, IDH and IKZF1. Leukemia. 24:1128–1138. 2010. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Characteristics of the four subtypes of myelodysplastic/myeloproliferative neoplasms

This article is mentioned in:

Abstract

Related Articles